Evaluation of<i>Pax5</i>expression and comparison with BLA.36 and CD79<i>α</i>cy in feline non‐Hodgkin lymphoma

Felisberto, Ricardo; Matos, Jesamar C.; Alves, Margarida; Cabeçadas, José; Henriques, Joaquim

doi:10.1111/vco.12262

Cited by 18 publications

(12 citation statements)

References 53 publications

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“…This is in contrast with human MCC, which often have immunoexpression of PAX‐5 6,19,20,30,31 . Although, PAX‐5 is not widely used as a B‐cell marker in cats, it has been reported to result in similar staining as in human and canine samples 36 . KIT expression was positive in all cases examined, which is in line with previous findings in canine and human MCC 16,18,34,35 .…”

Section: Resultssupporting

confidence: 87%

Feline and canine Merkel cell carcinoma: A case series and discussion on cellular origin

Steen

Grinwis

Weerts

et al. 2021

Vet Comparative Oncology

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Merkel cell carcinoma (MCC) is in humans and cats a malignant cutaneous neuroendocrine carcinoma, whereas in dogs it possibly has a more benign behaviour. It may be cytologically confused with round cell tumours such as lymphoma because of its striking cytomorphologic similarity. Although MCC is considered to arise from Merkel cells, recent findings indicated that primitive (epi‐)dermal stem cells, early B‐cells or dermal fibroblasts were the origin of human MCC. The aim of our study was to evaluate a possible lymphoid origin in feline and canine MCCs. Specific analysis of CD3, PAX‐5, KIT and PARR assay were performed in 3 feline and 3 canine MCCs. All MCCs (6/6) were negative for CD3 and PAX‐5. KIT was expressed in all MCCs (6/6). Assessment of clonality by PARR assay exhibited a polyclonal B‐ and T‐cell receptor rearrangement in all five cases tested. In conclusion, a lymphoid origin of feline and canine MCCs could not be demonstrated. This is in contrast with human MCCs, that often express early B‐cell lineage markers.

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Section: Resultssupporting

confidence: 87%

Feline and canine Merkel cell carcinoma: A case series and discussion on cellular origin

Steen

Grinwis

Weerts

et al. 2021

Vet Comparative Oncology

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“…It uses labelled antibodies to stain for the expression of specific cell membrane receptors found on different lymphocytes. Commonly used antibodies include CD3 for T cells, and CD79 for B cells (Waly and others 2005, Moore and others 2012), and a recent study has evaluated Pax5, a protein that is important in B cell differentiation, as an additional useful marker for B cell lymphoma (Felisberto and others 2017). This technique allows the pathologist to confirm that an identified round cell population is lymphocytic and shares the same phenotype (monomorphic population).…”

Section: Histopathologymentioning

confidence: 99%

Feline lymphoma: diagnosis, staging and clinical presentations

Mason¹,

Pittaway²

2022

In Practice

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Background: Lymphoma is a neoplasm of the lymphoid tissue, which may present with localised or systemic clinical signs. Unlike dogs that commonly present with multicentric disease, mostly involving the lymphoid organs (eg, lymph nodes), feline lymphoma can present as visceral (eg, spleen/liver/ gut), extranodal (eg, CNS/skin) or nodal in origin, with clinical presentation dependant on the affected organ. This variation in clinical presentation is further complicated by further indolent forms of lymphoma that have been reported; a cat can either present as clinically unwell with intrathoracic or intra-abdominal forms, or it can present with some subcutaneous or multicentric forms, which may be systemically asymptomatic.

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“…26,37,38 Other B-cell antigens have been utilized in more recent studies with evidence of increased specificity, including PAX5. 12,40 Protein expression of multiple myeloma oncogene 1 (MUM1), also called interferon regulatory factor 4 (IRF4), has been utilized with other T-and B-cell antigens in dogs and cats in the diagnosis of plasma cell tumors. 27 There are rare reports of lymphoproliferative diseases in which B-and T-cell antigens are co-expressed in domestic animals, and the panel of antibodies used is often variable and limited.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

A series of heterogeneous lymphoproliferative diseases with CD3 and MUM1 co-expressed in cats and dogs

et al. 2022

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Lymphoma diagnosis in dogs and cats is continually evolving as new subtypes and human correlates are being recognized. In humans, T-cell lymphomas with MUM1 expressed and plasma cell neoplasia or B-cell lymphomas with CD3 expressed aberrantly are reported only rarely. We report here a case series of tumors in dogs and cats with CD3 and MUM1 co-expressed as determined by immunocytochemistry or immunohistochemistry. Lineage was assigned for these tumors by 3 board-certified pathologists and a veterinary immunologist based on review of clinical and cellular features and the results of ancillary testing including PCR for antigen receptor rearrangements, flow cytometry, and serum protein electrophoresis with immunofixation. In cats, 7 of 7 tumors, and in dogs, 3 of 6 tumors with CD3 and MUM1 co-expressed had clonal rearrangement of the immunoglobulin gene or serum monoclonal immunoglobulin, consistent with a diagnosis of a plasma cell neoplasia or myeloma-related disorder with CD3 expressed aberrantly. Disease was often disseminated; notably, 3 of 7 feline cases had cutaneous and/or subcutaneous involvement in the tarsal area. In dogs, 3 of 6 cases had a clonal T-cell receptor gamma result and no clonal immunoglobulin gene rearrangement and were diagnosed as a T-cell tumor with MUM1 expressed. The use of multiple testing modalities in our series of tumors with plasma-cell and T-cell antigens in dogs and cats aided in the comprehensive identification of the lymphoproliferative disease subtype.

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Evaluation ofPax5expression and comparison with BLA.36 and CD79αcy in feline non‐Hodgkin lymphoma

Cited by 18 publications

References 53 publications

Feline and canine Merkel cell carcinoma: A case series and discussion on cellular origin

Feline and canine Merkel cell carcinoma: A case series and discussion on cellular origin

Feline lymphoma: diagnosis, staging and clinical presentations

A series of heterogeneous lymphoproliferative diseases with CD3 and MUM1 co-expressed in cats and dogs

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