Evaluation of immune effects of fowlpox vaccine strains and field isolates

Wang, Jianning; Meers, J.; Spradbrow, P. B.; Robinson, Wayne F.

doi:10.1016/j.vetmic.2006.03.012

Cited by 39 publications

(32 citation statements)

References 29 publications

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“…A final extension step was performed for 5 min at 72°C. The primers for the region of FWPV flanking REV 5 0 LTR as described by Wang et al [25] and the env gene [26] were used for the detection of REV integration. The PCR amplification conditions for both the genes were same with annealing temperature at 52°C.…”

Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%

Molecular Detection of Reticuloendotheliosis Virus (REV) Integration in Avian Poxvirus in North Eastern India

Puro

Ahuja

Joishy

et al. 2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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Avipoxviruses are responsible for decreased egg production, reduced growth and increased mortality in poultry. The disease has re-emerged as a new threat to poultry farmers as a number of outbreaks have been reported worldwide. Several workers have characterized the field strains of fowlpox virus and found incorporation of reticuloendotheliosis (REV) provirus in their genome. Here, the authors present the cases of poxvirus infection in three avian species viz., poultry, turkey and pigeon in north eastern India and found REV genes within the genome of poxvirus isolated from poultry and turkey. The presence of REV in the flocks of the region need to be further investigated as it reflects the emerging threats to the poultry industry at large and poultry rearing in the region in particular.

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Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%

Molecular Detection of Reticuloendotheliosis Virus (REV) Integration in Avian Poxvirus in North Eastern India

Puro

Ahuja

Joishy

et al. 2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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“…While the LTR remnants comprise about 200 or 500 bp, the near-fulllength provirus is about 8000 bp long and missing only a part of the 39 LTR (8,19). In most investigated field isolates the near-fulllength REV provirus or indications of its presence have been found, whereas REV sequences other than remnants of the LTR have been detected only in few vaccine strains (8,12,22).…”

mentioning

confidence: 98%

“…Since REV is an immunosuppressive virus, it is possible that the expression of REV genes influences the pathogenesis of fowlpox by impairing the immune response of the bird (20,22). This could be one possible explanation why antibody formation against FPV is infrequent (2,23,24).…”

mentioning

confidence: 99%

“…This could be one possible explanation why antibody formation against FPV is infrequent (2,23,24). FPV isolates carrying the near-full-length REV provirus were shown to have a reduced humoral and cellular immune response against antigens other than FPV (22). However there was no significant difference in the antibody response to FPV A Corresponding author.…”

mentioning

confidence: 99%

“…However there was no significant difference in the antibody response to FPV A Corresponding author. E-mail: lueschow.doerte@vetmed.fu-berlin.de AVIAN DISEASES 53:205-210, 2009 between chickens infected with field isolates and two vaccine strains with and without the near-full-length REV provirus (22). Similarly there was no correlation between antibody formation against REV and FPV (19).…”

mentioning

confidence: 99%

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Serologic Response Against Fowl Poxvirus and Reticuloendotheliosis Virus After Experimental and Natural Infections of Chickens with Fowl Poxvirus

et al. 2009

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Two infection studies in chickens were done to investigate the humoral immune response against fowl poxvirus (FPV) and reticuloendotheliosis virus (REV) after intradermal infection with different passages of a field isolate and with the vaccine strain HP B. The field isolate in a low passage carried the near-full-length REV provirus and induced antibodies to REV, but not to FPV. The vaccine strain carried only remnants of the long terminal repeat and induced antibodies against FPV, but not against REV. The field isolate lost the provirus after 36 passages in vitro, and it induced few antibodies against FPV and no antibodies against REV. Intravenous challenge with the low passage field isolate caused some antibody development against FPV in the birds that had previously been infected with the field isolate, but it caused no antibodies against REV in the previously vaccinated birds. REV proviral DNA was found in peripheral blood mononuclear cells of most birds that had been infected with the low passage field isolate. However, FPV DNA was found only once. The findings showed that the integrated REV provirus had an effect on the pathogenesis of fowlpox and that the tested vaccine strain is effective against FPV strains carrying REV provirus. Investigation of sera from FPV diseased flocks and flocks vaccinated against FPV showed a similar proportion of sera with antibodies against FPV. Sera from all diseased flocks but only from two of 10 vaccinated flocks had antibodies against REV. This indicated that the integrated REV provirus is common in FPV field strains.

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Pox

Tripathy¹,

Reed²

2019

Diseases of Poultry

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Evaluation of immune effects of fowlpox vaccine strains and field isolates

Cited by 39 publications

References 29 publications

Molecular Detection of Reticuloendotheliosis Virus (REV) Integration in Avian Poxvirus in North Eastern India

Molecular Detection of Reticuloendotheliosis Virus (REV) Integration in Avian Poxvirus in North Eastern India

Serologic Response Against Fowl Poxvirus and Reticuloendotheliosis Virus After Experimental and Natural Infections of Chickens with Fowl Poxvirus

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