Evaluation of Immunoblot Results for Determination of Antibodies to Lyme Disease Pathogens in Children of Ternopil Region

Abstract

Background. Lyme disease (LD) is a multisystem disorder caused by Borrelia burgdorferi and other similar tick-borne Borrelia. Objective. The aim of the research was to compare the results of the serological examination of children with different forms of Lyme disease. Methods. We observed the group of children (n=178) aged 1 to 14 years who were bitten by ticks. The control group consisted of 30 healthy children. Ticks were identified using a stereomicroscopic SEO system which included a stereomicroscope, a colour digital camera and a photoadapter. B. burgdorferi sensu lato (sl) (B. burgdorferi sensu stricto, B. afzeliі and B. garinii), B. miyamotoi, A. phagocytophilum DNA in blood were determined by real-time PCR. Baseline investigations related to clinical and immunological studies, including ELISA and Immunoblot, were performed. Results. The survey covered 178 child parents bitten by ticks. Borrelia burgdorferi sensu lato (B. afzelii, B. burgdorferi sensu stricto and B. garinii), B. miyamotoi and A. phagocytophilum were identified. Serological results in children with different forms of Lyme disease were compared. Conclusions. It is established that B. burgdorferi sensu lato; B. miyamotoi; and A. phagocytophilum are pathogens that cause erythema migrans in children. The presence of specific IgG (only positive results) to B. burgdorferi s.l. by immunoblot was confirmed in 83.8% of individuals who had positive and intermediate results in the ELISA test.

Clinical characteristics of patients with lyme arthritis diagnosed in children from Ternopil region

“…The probable prevalence of LA is 31% overall and 45% in the presence of knee triage. Children with joint damage caused by Lyme disease are more likely to experience knee lesions, lower peripheral leukocytes and lower cell count of fiber in the joints; they are less likely to have a fever compared to children suffering from septic arthritis [9,10]. In our study, all patients had persistent pain joint syndrome.…”

Features of the Algorithm of Clinical and Laboratory Diagnosis of Lyme Borreliosis in Children

“…These months are likely to be the time of the tick breeding cycle and the peak time for people to spend outdoors. According to scientific observations, the skin of the lower extremities, buttocks, abdomen and head in children are the most common areas of tick bites [6]. Most often, the bites were localised in the head (19 -30.6 %) and lower extremities (18 -29.03 %), trunk (9 -14.51 %), neck (8 -12.90 %), upper extremities (2 -3.22 %), abdomen (4 -6.44 %), and earlobe (2 -3.22 %) in the study group of children, which was confirmed by other studies [6,7].…”

Особливості Лабораторних Досліджень Еритемних Та Безеритемних Форм Хвороби Лайма У Дітей

“…Оцінка серединних рівнів СРП у пацієнтів встановила, що цей показник виявився найвищим у пацієнтів групи 3 і становив 5,0 [3,9; 12,0] мг/л, достовірно відрізняючись від такого як у пацієнтів групи 1 (р=0,02), де він склав 1,4 [0,6; 3,7] мг/л, так і групи 2 (р=0,02), у пацієнтів якої серединний рівень СРП становив 2,1 [1,5; 2,4] [13]. У дослідженні, проведеному у Тернополі, теж виявлено високі значення антитіл IgG до B. burgdorferi у пацієнтів з ранньою та пізньою дисемінованими стадіями ХЛ [14].…”