Evaluation of immunological markers in serum, filter-paper blood spots, and saliva for dengue diagnosis and epidemiological studies

Balmaseda, Ángel; Saborío, Saira; Téllez, Yolanda; Mercado, Juan Carlos; Pérez, Lissette; Hammond, Samantha N.; Rocha, Crisanta; Kuan, Guillermina; Harris, Eva

doi:10.1016/j.jcv.2008.07.016

Cited by 60 publications

(49 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Statistical significance was P 0.05. infection may be related to inefficient detection of NS1 during secondary infections. [35][36][37][38] In summary, these results suggest that during primary infections, levels of circulating NS1 are higher in patients with DHF than in patients with DF. Nonetheless, our results and other results 18,20 also suggest that concentrations of circulating NS1 are affected by the infecting serotype and the immunologic status (i.e., primary or secondary infection) of the patient.…”

Section: Discussionmentioning

confidence: 72%

Determination of Viremia and Concentration of Circulating Nonstructural Protein 1 in Patients Infected with Dengue Virus in Mexico

Cruz-Hernández¹,

Flores-Aguilar²,

González-Mateos³

et al. 2013

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Higher levels of viremia and circulating nonstructural protein 1 (NS1) have been associated with dengue disease severity. In this study, viremia and circulating NS1 levels were determined in 225 serum samples collected from patients in Mexico infected with dengue virus serotypes 1 and 2 (DENV-1 and DENV-2). Patients with dengue hemorrhagic fever (DHF) who were infected with DENV-1 showed higher levels of circulating NS1 than patients with dengue fever (DF) (P = 0.0175). Moreover, NS1 levels were higher in patients with primary infections with DENV-1 than in patient infected with DENV-2 (P 0.0001) and in patients with primary infections with DENV-2 than in patients with secondary infections with DENV-2 (P = 0.0051). Unexpectedly, viremia levels were higher in patients with DF than in those with DHF infected with either DENV-1 or DENV-2 (P = 0.0019 and P = 0.001, respectively) and in patients with primary infections than those with secondary DENV-2 infections (P 0.0001). Results indicate that levels of circulating NS1 vary according to the infecting serotype, immunologic status (primary or secondary infection), and dengue disease severity.

show abstract

Section: Discussionmentioning

confidence: 72%

Determination of Viremia and Concentration of Circulating Nonstructural Protein 1 in Patients Infected with Dengue Virus in Mexico

Cruz-Hernández¹,

Flores-Aguilar²,

González-Mateos³

et al. 2013

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

“…Blood samples were obtained from all children at enrollment (during November 2008-January 2009) and one year after enrollment (during November 2009-January 2010) by finger prick and stored as blood spots on protein saver cards (Whatman, Piscataway, NJ and Id Biological Systems, Greeneville, SC). 24,25 From children with a documented fever, whole blood was collected by venipuncture and placed in tubes containing EDTA. Some of the whole blood was used to prepare dried blood spots for serologic testing.…”

Section: Methodsmentioning

confidence: 99%

Burden of Dengue Infection and Disease in a Pediatric Cohort in Urban Sri Lanka

Tissera¹,

Amarasinghe²,

Silva³

et al. 2014

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Dengue is the most significant arthropod-borne viral infection of humans. Persons infected with dengue viruses (DENV) have subclinical or clinically apparent infections ranging from undifferentiated fever to dengue hemorrhagic fever/shock syndrome. Although recent studies estimated that the Indian subcontinent has the greatest burden of DENV infection and disease worldwide, we do not have reliable, population-based estimates of the incidence of infection and disease in this region. The goal of this study was to follow-up a cohort of 800 children living in a heavily urbanized area of Colombo, Sri Lanka to obtain accurate estimates of the incidence of DENV infection and disease. Annual blood samples were obtained from all children to estimate dengue seroprevalence at enrollment and to identify children exposed to new DENV infections during the study year. Blood was also obtained from any child in whom fever developed over the course of the study year to identify clinically apparent DENV infections. At enrollment, dengue seroprevalence was 53.07%, which indicated high transmission in this population. Over the study year, the incidence of DENV infection and disease were 8.39 (95% confidence interval = 6.56-10.53) and 3.38 (95% confidence interval = 2.24-4.88), respectively, per 100 children per year. The ratio of clinically inapparent to apparent infections was 1.48. These results will be useful for obtaining more accurate estimates of the burden of dengue in the region and for making decisions about testing and introduction of vaccines.

show abstract

“…Diagnosis of other viral infections in urine and saliva samples has been reported for human immunodeficiency virus (HIV), Hepatitis A, B, and C, and rubella [14][15][16][17][18][19][20], and more recently in other flaviviruses, West-Nile virus (WNV) [21][22][23] and Dengue (DENV) serotypes -1, -2, -3, and -4 [24][25][26][27][28][29][30][31][32][33][34][35]. WNV can be detected longer in urine than serum [21] and can be isolated from urine [22,23].…”

Section: Advantages Of Non-invasive Sample Collectionsmentioning

confidence: 99%