Evaluation of Immunomodulatory Activity of the Herbals Formula Viranur, Turmeric (Curcuma heyneana Val.) and Phyllanthus (Phyllanthus niruri L.) in Layer Chicken Vaccinated with Avian Influenza

Abstract

Abstract:The aim of this research was to know the effect of herbal as immunomodulator on chicken layer vaccinated with avian influenza. A total of 60 chickens were alloted into three treatment groups: control group (KA), group KB and group KC, with 20 chicken each group. All the chickens were vaccinated with Newcastle disease (ND) in the age of one week, and a week later they were vaccinated with avian influenza (AI). The chickens in group KB were drunken with herbal solution containing of 5 g turmeric (Curcuma heyneana Val.) and 25 g phyllanthus (Phyllanthus niruri L.) and group KC were drunken with herbal solution containing of 36 g the herbals formula Viranur and 25 g phyllanthus (Phyllanthus niruri L.), respectively for four weeks. Thirty days after AI vaccinated, all of chicken were weighed and necropsied. Samples from bursa of Fabricius, tymus and spleen were taken for weighing and histopathological examination. The weight indexs of bursa of Fabricius, tymus and spleen were not significantly different between control group and treatment group in the considered statistically significance (P > 0.05), but the treatment groups (KB and KC) had higher weight index. The histopathologically changes of spleen in both control group and treatment groups were not different, although in the group KC, in bursa of Fabricius, there was lymphocyte increase in its lymphoid follicles; and in the group KB and KC, the tymus were more widening in the cortex than medulla. The conclusion of this study showed that the herbals can stimulate lymphocyte activity.

Evaluation of the Effect of Hydroethanolic Root Extract and Solvent Fractions of Cyphostemma adenocaule (Steud. ex A. Rich) Descoings ex Wild & Drummond on Cell-Mediated Immune Response and Blood Cell Count in Mice

“…Hydroethanolic root extract at a dose of 400 mg/kg induced the highest spleen index (5.359 ± 0.174). The increase in organ index might also be due to the stimulatory effect of treatment on proliferation and differentiation of bone marrow stem cells that migrate to and settle within the organ for interaction with the incoming antigen, thereby increasing the mass of the organ [ 20 ].…”

Gangguan Pertumbuhan Organ Limfoid Ayam Broiler yang Menderita Omfalitis

“…Penentuan indeks berat organ limfoid seperti bursa Fabricius, timus, dan limpa dilakukan dengan menimbang masing-masing berat badan, berat organ limfoid, bursa Fabricius timus, dan limpa seperti dilansir (Hartati, et al, 2015) sebagai berikut: dimana: Bobot organ limfoid = bobot tiap organ limfoid Bobot Ayam = bobot tiap ayam…”

“…Sebagai perbandingan, organ timus kontrol normal menunjukkan korteks timus tampak limfosit tersusun lebih padat dan tidak terlihat vakuolisasi(Gambar 8). Faktor kedua adalah imunomodulator emberian imunomodulator pada ayam yang telah di vaksinasi menunjukkan kepadatan limfosit tinggi pada organ limfoid dan meningkatkan bobot organ limfoid (Hartati et al, 2015). Indeks berat organ limfoid pada ayam omphalitis lebih rendah dibandingkan ayam sehat karena terjadi kematian atau kerusakan sejumlah limfosit pada folikel.…”

EARLY STUDY THE POTENCY OF TURMERIC (Curcuma domestica Val.) AS IMMUNOSTIMULATOR FOR LAYERS CHICKENS AGAINST AVIAN INFLUENZA (AI) VACCINE

“…All groups were challenged with H9N2 Avian Influenza virus, and the treatment group showed mild clinical symptoms, the lowest viral shedding and higher titer than the control group. Research conducted by Hartati et al [3] showed that giving curcumin in layer chicken feed stimulates lymphocyte activity. In the histopathological picture of the bursa fabricius, there was an increased number of lymphocytes in the lymphoid follicles and the thymus were also seen in widening of the cortex compared to the medulla.…”