Evaluation of Immunomodulatory Effects of Mesenchymal Stem Cells Soluble Factors on miR-155 and miR-23b Expression in Mice Dendritic Cells

Karachi, Aida; Fazeli, Mehdi; Karimi, Mohammad Hossein; Geramizadeh, Bita; Moravej, Ali; Ebrahimnezhad, Salimeh; Afshari, Afsoon

doi:10.3109/08820139.2015.1017046

Cited by 15 publications

(12 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, it was observed that mir-126, PG2 treatment had no significant effect on mir-23 expression compared to the control group. In this line, Wu et al (2017) showed that miR-23b overexpression inhibited activation of the NF-jB pathway, so mir-23 may have a positive regulatory effect on the immunoregulatory property of MSCs (Karachi et al, 2015, Wu et al, 2017. According to our results, it can be expected that the upregulation of mir-23 by PGEs treatment can improve the immunoregulatory properties of Ad-MSCs.…”

Section: Discussionsupporting

confidence: 54%

“…MSCs have gained extensive recognition and international attention because of their immunomodulatory properties and use of this capacity for the treatment of autoimmune and degenerative diseases (Kuo et al, 2012, Willis et al, 2018. In addition, miRNAs can influence the immune-regulatory activities of MSCs through targeting various modulatory genes, as a positive regulator or negative regulator (Xu et al, 2013;Karachi et al, 2015;Wu et al, 2015;Khosravi et al, 2017;Onodera et al, 2017;Wu et al, 2017) In addition, studies have shown that PG has different properties such as anti-inflammatory effects by modulating some miRNAs expression and this target gene, which plays a role in the inflammatory pathway (Banerjee et al, 2012(Banerjee et al, , 2013. So, in this study, we assess the impact of PGEs on immunoregulatory-related miRNAs expression in Ad-MSCs for the first time.…”

Section: Discussionmentioning

confidence: 99%

“…miRNAs are important in immune response regulation (O'connell et al, 2010). In this regard, it has been reported that miRNAs by modulation the targets can affect the immunoregulatory properties of MSCs in different manners such as positive effect on immunoregulatory activity of MSCs (positive regulator) for instance (mir-126 and mir-23) (Karachi et al, 2015;Khosravi et al, 2017;Wu et al, 2017) or negative effect on immunoregulatory activity of MSCs (negative regulator) for instance (mir-155 and mir-21) (Xu et al, 2013;Wu et al, 2015;Onodera et al, 2017). Therefore, changing the expression of miRNAs in MSCs can influence the immunoregulatory properties of MSCs (Chi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Pomegranate Extract in Mesenchymal Stem Cells by Modulation of microRNA-155, microRNA-21, microRNA-23b, microRNA-126a, and PI3K\AKT1\NF-κB Expression

Rostami

Khorashadizadeh

Ghoncheh

et al. 2020

DNA and Cell Biology

View full text Add to dashboard Cite

Today, mesenchymal stem cells (MSCs) are candidates for various autoimmune disease treatments due to immunomodulatory activity in these cells. Much research has recently been done to improve the immunomodulatory activity of MSCs. Genetic variation is one of these methods. microRNAs (miRNAs) are small noncoding RNAs that control most of the cell's biological activities. Recent studies have shown that miRNAs play a significant role in the regulation of MSC immunomodulatory activity. Pomegranate is a fruit that has antioxidant, anti-inflammatory, and anticancer properties and has been used for many years for therapeutic purposes. The objective of this research is to evaluate the immunoregulatory-related miRNAs level of adiposederived MSCs (Ad-MSCs) obtained from adipose tissue in the presence or lack of pomegranate (Punica granatum) extract (PGE). Our results showed that miRNA-23 and miRNA-126 were upregulated by PGE treatment in MSCs, and in contrast, miRNA-21 and miRNA-155 were downregulated by PGE treatment in MSCs. In addition this research shows that PGE can downregulate the expression of PI3K\AKT1\NF-jB in Ad-MSCs. Our bioinformatics data have shown that the target of these four miRNAs and the signaling pathways, in which these targets are involved, can play an important role in regulating the immunomodulation function of stem cells. In conclusion, PGE can inhibit the expression of PI3K\AKT1\NF-jB genes involved in inflammatory pathways via miRNA-23 and miRNA-126 overexpression or miRNA-21 and miRNA-155 downregulation that plays a role in the pathways of immune modulation in Ad-MSCs. These results may provide insight into the mechanism underlying the regulation of the immunomodulatory activity of Ad-MSCs by PGE.

show abstract

Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Pomegranate Extract in Mesenchymal Stem Cells by Modulation of microRNA-155, microRNA-21, microRNA-23b, microRNA-126a, and PI3K\AKT1\NF-κB Expression

Rostami

Khorashadizadeh

Ghoncheh

et al. 2020

DNA and Cell Biology

View full text Add to dashboard Cite

show abstract

“…Allogeneic GVAX vaccine has been studied for treatment of prostate cancer [42] [49]. DCs act as a double-edged sword that can induce both immune tolerance and immune activation depending on which receptors on DCs are engaged [50]. Maturation and migration of DCs play a critical role in characteristics of DCs [51].…”

Section: Cancer Vaccinesmentioning

confidence: 99%

Immunotherapy for Treatment of Cancer

Karachi¹

2019

Current Trends in Cancer Management

Self Cite

View full text Add to dashboard Cite

Cancer is known to be second cause of death worldwide despite aggressive therapeutic measures such as surgical resection of tumors, radiation therapy, and chemotherapy. The failure of currently available therapeutics for cancers, has led to increasing interest in alternative approaches including immunotherapy. Immunotherapy for cancer treatment is enhancing immune responses to fight cancer cells. Monoclonal antibodies, immune checkpoint blockades, targeted therapy, adoptive cell therapy, CAR T cells, and cancer vaccines are the most current and efficient parts of immunotherapy armamentarium. Immunotherapy has tremendous success in the treatment of cancers and is considered as a standard care of treatment or recurrence preventive therapy for variety of cancers. In this chapter, we discuss different types of immunotherapy for cancer treatment in detail.

show abstract

“…Mesenchymal stem cells soluble factors could modulate some speci c miRs expression, like those of miR-155 and miR-23b in dendritic cells (23). Whether miRs are involved in underlying mechanism of CD4 + T cells in response to MSCs upon hypoxic and in ammatory condition during ARDS needs further investigation.…”

mentioning

confidence: 99%

Mesenchymal Stem Cells-Secreted TGF-β1 Restores Treg/Th17 Skewing Induced by Lipopolysaccharide and Hypoxia Challenge via miR-155 Suppression

Xue

Zhang

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Regulatory T cells (Treg)/T helper (Th) 17 skewing plays a crucial role in development of acute respiratory distress (ARDS). Mesenchymal stem cells (MSCs)-secreted transforming growth factor (TGF)-β1 has remarkable immunomodulatory effects on CD4+T cells, being environment sensitive and remains lack of discussion in hypoxic and inflammatory condition of ARDS.Methods: Purified mice splenic CD4+ T cells were pre-coated with anti-CD3 (5ug/ml) and anti-CD28 (2ug/ml) overnight. RAW264.7 cells were added as antigen presenting cells (APCs). T cells with and without RAW264.7 cells were treated with various LPS concentrations of 0,10,100,1000ng/ml or/and at hypoxia condition of 5% O2. Based on LPS (100ng/ml) and hypoxia condition (5% O2) as stimuli, MSCs were set in direct coculture or indirect coculture methods by transwell system. Anti-TGF-β1 neutralization antibody was added to explore the role of TGF-β1 among the soluble factors secreted by MSCs; miR-155 overexpression of CD4+T cells were performed by transfection and then were added to the MSCs-CD4+T cells coculture system in hypoxic and LPS-stimulated condition. After 48 hours, cells or supernatant were collected for detection of frequency of Treg and Th17 subsets, CD4+T cells apoptosis and proliferation capacity assay by flowcytometry, secretions of INF-γ, IL-17A, IL-21, TGF-β1 and IL-10 by ELISA, levels of miR-155, Rorc, Foxp3 and Ptpn2 mRNA expression of CD4+T cells by RT-PCR.Results: MSCs could restore the skewed Treg/Th17 induced by LPS and hypoxia as compared to groups without MSCs with increased secretion of TGF-β1, IL-10 and IL-17A(P<0.05) and attenuate the increased expression of miR-155 in CD4+T cells, which was independent on cell-to-cell contact mechanism while TGF-β1 neutralization could significantly inhibit the effects of MSCs restoring the skewed Treg/Th17 and abolished its effect on miR-155 expression in CD4+T cells.Conclusions: These findings suggested miR-155 suppression of CD4+T cells mediated MSCs-secreted TGF-β1 modulating the skewed Treg/Th17 induced by LPS-hypoxia challenge, providing evidence when proposing future T lymphocyte-targeted cell therapy in ARDS.

show abstract

Evaluation of Immunomodulatory Effects of Mesenchymal Stem Cells Soluble Factors on miR-155 and miR-23b Expression in Mice Dendritic Cells

Cited by 15 publications

References 33 publications

Effect of Pomegranate Extract in Mesenchymal Stem Cells by Modulation of microRNA-155, microRNA-21, microRNA-23b, microRNA-126a, and PI3K\AKT1\NF-κB Expression

Effect of Pomegranate Extract in Mesenchymal Stem Cells by Modulation of microRNA-155, microRNA-21, microRNA-23b, microRNA-126a, and PI3K\AKT1\NF-κB Expression

Immunotherapy for Treatment of Cancer

Mesenchymal Stem Cells-Secreted TGF-β1 Restores Treg/Th17 Skewing Induced by Lipopolysaccharide and Hypoxia Challenge via miR-155 Suppression

Contact Info

Product

Resources

About