Evaluation of impairment of DNA integrity in marine gastropods (Cronia contracta) as a biomarker of genotoxic contaminants in coastal water around Goa, West coast of India

Sarkar, A.; Gaitonde, D.C.S.; Sarkar, Amit; Vashistha, D.; D'Silva, C.; Dalal, S.G.

doi:10.1016/j.ecoenv.2008.01.006

Cited by 26 publications

(11 citation statements)

References 51 publications

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“…The usefulness of gastropods as sentinel organism in metal biomonitoring studies are widely recognized (Downs et al, ; Sarkar et al, ; Itziou and Dimitriadis ). Gill cells are used as an attractive cellular model in ecotoxicology; as the gills are constantly exposed to dissolved contaminants and are capable of metabolizing carcinogens and mutagens into reactive products (Mitchelmore et al, ; Wilson et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…The physicochemical parameters such as temperature, pH, salinity, dissolved oxygen (DO), biochemical oxygen demand (BOD), and nutrients (nitrate, nitrite, and phosphate) were determined following the standard procedure (Grasshoff et al, ). The concentration of cadmium in the tissues of a marine gastropod, Cronia contracta , from the same sampling station (Palolem) was found to be in the range, 0.1±0.03 to 0.2 ± 0.03 µg/g (dry weight) (Sarkar et al, ). Moreover, the concentration of cadmium in water from the Goa coastal region was estimated to be 0.24 µg/L (KrishnaKumari et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…The DNA alkaline unwinding assay (DAUA) was used to detect DNA damage caused by complex environmental contamination in aquatic test organisms (Ahmad et al, ; Sarkar et al, ; Pisanelli et al, ; Bechmann et al, ; Oliveira et al, ). This assay enables the assessment of primary DNA damage in tissue from exposed aquatic test organisms.…”

Section: Introductionmentioning

confidence: 99%

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Genotoxicity of cadmium chloride in the marine gastropod Nerita chamaeleon using comet assay and alkaline unwinding assay

Sarkar

Bhagat

Ingole

et al. 2013

Environmental Toxicology

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This paper presents an evaluation of the genotoxic effects of cadmium chloride (CdCl2 ) on marine gastropod, Nerita chamaeleon following the technique of comet assay and the DNA alkaline unwinding assay (DAUA). In this study, the extent of DNA damage in gill cells of N. chamaeleon was measured after in vivo exposure to four different concentrations (10, 25, 50, and 75 µg/L) of CdCl2 . In vitro exposure of hydrogen peroxide (H2 O2 ; 1, 10, 25, and 50 µM) of the gill cells showed a significant increase in the percentage tail DNA, Olive tail moment, and tail length (TL). Significant changes in percentage tail DNA by CdCl2 exposure were observed in all exposed groups of snails with respect to those in control. Exposure to 75 µg/L of CdCl2 produced significant decrease in DNA integrity as measured by DAUA at all duration with respect to control. In vivo exposure to different concentrations of CdCl2 (10, 25, 50, and 75 µg/L) to N. chamaeleon showed considerable increase in DNA damage as observed by both alkaline comet assay and the DAUA. The extent of DNA damage in marine gastropods determined by the application of alkaline comet assay and DAUA clearly indicated the genotoxic responses of marine gastropod, N. chamaeleon to a wide range of cadmium concentration in the marine environment.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Genotoxicity of cadmium chloride in the marine gastropod Nerita chamaeleon using comet assay and alkaline unwinding assay

Sarkar

Bhagat

Ingole

et al. 2013

Environmental Toxicology

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“…However, the induction of single-strand breaks by xenobiotic agents or genotoxicants is mediated for example, by chemical induction during excision repair, interaction with DNAintercalating agents, degradation due to autolysis or disruption, formation of alkali labile sites [9,11,12]. In particular, significant DNA damage has been observed by alkylating agents which can interact with DNA at multiple sites leading to single-strand breaks [7,13].…”

Section: Introductionmentioning

confidence: 99%

“…They inflict detrimental damage to the biologic integrity and physiologic functions of individual organisms. A part of the potentially toxic material is readily accumulated within the tissues of resident organisms from surrounding environment and thereby cause severe damage into their physiological status [1][2][3][4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Role of Marine Pollutants in Impairment of DNA Integrity

Sarker¹,

Sarkar²

2015

J Clin Toxicol

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In this article, we present an overview on the role of marine pollutants in impairment of DNA integrity in marine gastropods exposed to xenobiotics released from various sources into the coastal ecosystem. We provide an insight into the impact of various types of genotoxic compounds on the physiologic status of marine organisms, with specific focus on DNA integrity. DNA damage in marine gastropods was found to be highly correlated to the level of contaminants prevalent in the marine environment. DNA integrity in marine organisms was significantly affected upon exposure to genotoxicants such as polycyclic aromatic hydrocarbons (PAH), persistent organic pollutants, cadmium, lead, manganese, chromium, copper, etc. The prevalence of persistent xenobiotic contaminants in the marine environment coupled to their deleterious impact on marine organisms, exemplifies the measurement of DNA integrity as a prognostic tool for marine pollution assessment, control and policy-making.

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