Methods for Genotoxicity Testing of Environmental Pollutants

Masood, Farhana; Anjum, Reshma; Ahmad, Masood; Malik, Abdul

doi:10.1007/978-94-007-1591-2_7

Cited by 5 publications

(3 citation statements)

References 138 publications

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“…Following staining, the chromosomes are analyzed microscopically for aberrations. FISH-staining techniques have been used to increase the sensitivity of CA, allowing each chromosome to be differentially stained to reveal chromosomal rearrangements not detectable with conventional staining techniques (70,71).…”

Section: Chromosomal Aberration (Ca) Assaymentioning

confidence: 99%

Models for Detection of Genotoxicity in vivo: Present and Future

Musgrove¹,

Camps²

2012

Mutagenesis

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Section: Chromosomal Aberration (Ca) Assaymentioning

confidence: 99%

Models for Detection of Genotoxicity in vivo: Present and Future

Musgrove¹,

Camps²

2012

Mutagenesis

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“…Pesticides are from these pollutants, which used in agriculture. Organophosphrous (OPs) are among the most common pesticide classes used in crop and livestock protection with high toxicity [1]. Chloropyrifos is one of the most OPs used in agriculture, as it used in cotton, corn, almonds, and fruit trees including oranges and apples [2,3].…”

Section: Introductionmentioning

confidence: 99%

A Novel Chloropyrifos Electrochemical Sensor Based on Polyaniline/Carbon Nanotubes Composite

Ebrahim

El-Raey

Hefnawy

et al. 2014

KEM

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Polyaniline nanofibers were prepared chemically in both emiraldine base (EB) and emiraldine salt (ES) forms. The composite of polyaniline emiraldine salt with single walled carbon nanotubes (SWCNTs) was developed. Electrochemical sensors based on polyaniline nanofibers and its composite have been developed for the detection of chloropyrifos. Chloropyrifos exhibits one well defined reduction peak. The maximum peak current was linearly related to chloropyrifos concentration in the range from 0.2 μM to 1.4 μM. It was found that the SWCNTs improved the sensitivity of the polyaniline base graphite electrode from 3.0 mA/μM for polyaniline base to 9.69 mA/μM for ES-SWCNTs composite electrode.

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“…Among short-term toxicity assays, the comet assay is a very sensitive test for the quantification of DNA damage. Although DNA damage as revealed by the comet assay may not necessarily result in permanent genetic damage, there is consensus about a close association of unrepaired DNA damage or error-prone repair processes, mutations, and the induction of various types of cancer [21]. In view of the above considerations, and due to the lack of information about Cassia alata genotoxicity, it is necessary to perform investigation on the effects of this herbal product which is often used as a therapeutic modality on genetic alterations.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Bronchorelaxant, Genotoxic, and Antigenotoxic Effects ofCassia alataL.

Ouédraogo

Fabre

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Aqueous-ethanolic extract of Cassia alata (AECal) and its derived fractions obtained through liquid-liquid fractionation were evaluated for their bronchorelaxant, genotoxic, and antigenotoxic effects. Contractile activity of rats' tracheas in the presence of tested materials, as well as its modifications with different inhibitors and blockers, was isometrically recorded. The antigenotoxic potential of AECal was evaluated on cyclophosphamide- (CP-) induced genotoxicity in the rat. Animals were pretreated with the extract, then liver comet assay was performed. AECal and its chloroformic fractions (CF-AECal) relaxed the contraction induced by Ach, but both were significantly less potent in inhibiting contraction induced by KCl (30 mM; 80 mM). Propranolol, indomethacin, L-NAME, methylene blue, and glibenclamide did not modify the relaxant effect of CF-AECal. TEA altered the response of trachea to CF-AECal. CF-AECal caused a rightward shift without affecting the E max in cumulative concentration-response curves of Ach only at low concentrations. In animals pretreated with the extract, the percentage of CP-induced DNA damage decreased. Our results suggest that (1) muscarinic receptors contribute at least in part to the relaxant effects of CF-AECal; (2) CF-AECal interferes with membrane polarization; and (3) AECal is not genotoxic in vivo and contains chemopreventive phytoconstituents offering protection against CP-induced genotoxicity.

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Methods for Genotoxicity Testing of Environmental Pollutants

Cited by 5 publications

References 138 publications

Models for Detection of Genotoxicity in vivo: Present and Future

Models for Detection of Genotoxicity in vivo: Present and Future

A Novel Chloropyrifos Electrochemical Sensor Based on Polyaniline/Carbon Nanotubes Composite

Evaluation of the Bronchorelaxant, Genotoxic, and Antigenotoxic Effects ofCassia alataL.

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