Evaluation of imprint DESI-MS substrates for the analysis of fungal metabolites

Tata, Alessandra; Pérez, Carlos; Ore, Moriam O.; Lostun, Dragos; Passas, Aristides; Morin, Sylvie; Ifa, Demian R.

doi:10.1039/c5ra12805f

Cited by 26 publications

(28 citation statements)

References 38 publications

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“…(D.a) m/z 561; (D.b) m/z 585; and (D.c) m/z 614. [(A)–(D) reproduced from Li et al, Tata et al, and Angolini et al, respectively]…”

Section: Recent Applications In Ambient Ms Imagingmentioning

confidence: 97%

“…Silicon surfaces gave the highest signal intensity, while filter paper and PTFE could maintain stable signals for a longer duration. Imprinting on double‐sided tape was found beneficial for performing DESI‐MS imaging of fungal cultures (Figure B) . Furthermore, Tata and coworkers employed this established protocol with double‐sided tape to study the chemical interaction between the phytopathogen Moniliophthora roreri and the endophyte Trichoderma harzianum .…”

Section: Recent Applications In Ambient Ms Imagingmentioning

confidence: 99%

“…Indirect imaging of plant tissues by imprinting has been investigated on substrates such as paper, porous PTFE surfaces, cellulose ester filter membranes, and TLC plates . The imprinting approach consists of slicing the plant tissue and blotting onto hard, absorbent substrates so that molecules are extracted and transferred to create a replicate of the top layer of the plant tissue onto the substrate .…”

Section: Recent Applications In Ambient Ms Imagingmentioning

confidence: 99%

“…Ambient MS imaging has matured to such an extent that biologically relevant issues can be solved in a multitude of natural product applications. Recently, research has been focused on the 113 Tata et al, 106 and Angolini et al, 110 respectively] developmental stages of plant and seed growth, 117,118 the onset and production of plant metabolites, [119][120][121][122][123] ecological significance and function, 118,124 and pathogenic and pesticide control strategies. 119,125 In the direct analysis of plant tissues, the tissues are mounted directly onto the target plate without further manipulation.…”

Section: Plants and Natural Product Metabolomicsmentioning

confidence: 99%

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Review and perspectives on the applications of mass spectrometry imaging under ambient conditions

Pérez

Bagga

Prova

et al. 2018

Rapid Comm Mass Spectrometry

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Ambient mass spectrometry (AMS)-based techniques are performed under ambient conditions in which the ionization and desorption occur in the open environment allowing the direct analysis of molecules with minimal or no sample preparation. A selected group of AMS techniques demonstrate imaging capabilities that can provide information about the localization of molecules on complex sample surfaces such as biological tissues. 2D, 3D, and multimodal imaging have unlocked an array of applications to systematically address complex problems in many areas of research such as drug monitoring, natural products, forensics, and cancer diagnostics. In the present review, we summarize recent advances in the field with respect to the implementation of new ambient ionization techniques and current applications in the last 5 years. In more detail, we mainly focus on imaging applications in topics related to animal whole bodies and tissues, single cells, cancer diagnostics and biomarkers, microbial cultures and co-cultures, plant and natural product metabolomics, and forensic applications. Finally, we discuss new areas of research, future perspectives, and the overall direction that the field may take in the years to come.

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“…(D.a) m/z 561; (D.b) m/z 585; and (D.c) m/z 614. [(A)–(D) reproduced from Li et al, Tata et al, and Angolini et al, respectively]…”

Section: Recent Applications In Ambient Ms Imagingmentioning

confidence: 97%

Section: Recent Applications In Ambient Ms Imagingmentioning

confidence: 99%

Section: Recent Applications In Ambient Ms Imagingmentioning

confidence: 99%

Section: Plants and Natural Product Metabolomicsmentioning

confidence: 99%

See 2 more Smart Citations

Review and perspectives on the applications of mass spectrometry imaging under ambient conditions

Pérez

Bagga

Prova

et al. 2018

Rapid Comm Mass Spectrometry

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“…[18][19][20] Such characteristics have therefore become DESI-MS, a potential technique in imaging of biological samples. Specifically in microbiology studies, DESI-MSI has been applied in the characterization of different types of microorganisms, [21][22][23][24] in studies of microbial interactions 16,25,26 and interactions of microorganisms with their hosts, 27 allowing the determination of the nature of antibiotics and their localizations at the surface of the colonies. 16,26,28,29 In this report, we have used DESI-MSI, allied to the electrospray ionization tandem mass spectrometry (ESI-MS/MS), to spatially monitor and characterize the metabolites produced by Curtobacterium sp.…”

Section: Introductionmentioning

confidence: 99%

Mass spectrometry characterization of endophytic bacterium Curtobacterium sp. strain ER1/6 isolated from Citrus sinensis

et al. 2017

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The bacteria of the genus Curtobacterium are usually seen as plant pathogen, but some species have been identified as endophytes of different crops and could as such present a potential for disease control and plant growth promotion. We have therefore applied the desorption electrospray ionization mass spectrometry imaging (DESI-MSI) in the direct analysis of living Curtobacterium sp. strain ER1/6 colonies to map the surface metabolites, and electrospray ionization tandem mass spectrometry (ESI-MS/MS) for characterization of these compounds. Several colony-associated metabolites were detected. The ESI-MS/MS showed characteristic fragmentations for phospholipids including the classes of glycerophosphocholine, glycerophosphoglycerol, and glycerophosphoinositol as well as several fatty acids. Although a secure identification was not obtained, many other metabolites were also detected for this bacteria species. Principal component analysis showed that fatty acids were discriminatory for Curtobacterium sp. ER1/6 during inoculation on periwinkle wilt (PW) medium, whereas phospholipids characterize the bacterium when grown on the tryptic soy agar (TSA) medium. Recently, the fast characterization of microbial secondary metabolite biomarkers by desorption electrospray ionization mass spectrometry (DESI-MS) has rapidly increased. 14-17 The DESI-MS is the combination of electrospray ionization mass spectrometry (ESI-MS) and direct sample desorption using a flow of electrosprayed charged droplets bombarding a surface to be analyzed; therefore, it is also applicable to solid samples such as complex biological materials. allowing the determination of the nature of antibiotics and their localizations at the surface of the colonies. 16,26,28,29 In this report, we have used DESI-MSI, allied to the electrospray ionization tandem mass

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Desorption Electrospray Ionization Mass Spectrometry Imaging for Tissue Analysis

Song

Chen

2020

Encyclopedia of Analytical Chemistry

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Mass spectrometry imaging (MSI) has become a significant technique of mass spectrometry (MS) to visualize the spatial distribution of molecules such as drugs, metabolites, lipids, and proteins by their molecular masses. As a representative ambient ionization method that requires minimal or no sample preparation, desorption electrospray ionization mass spectrometry (DESI‐MS) has been shown to have its unique strength in MSI. This article provides an overview of this powerful DESI‐MSI technique, including its basic principle, instrumentation, sample preparation, method optimization, 2D and 3D imaging, quantitation, statistical analysis, software, and its applications in pharmaceutical, clinical, biomedical, plant, microbial, and forensic researches.

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Evaluation of imprint DESI-MS substrates for the analysis of fungal metabolites

Abstract: Optimized in situ screening, characterization and imaging of fungal metabolites by imprint DESI-MS.

Cited by 26 publications

References 38 publications

Review and perspectives on the applications of mass spectrometry imaging under ambient conditions

Review and perspectives on the applications of mass spectrometry imaging under ambient conditions

Mass spectrometry characterization of endophytic bacterium Curtobacterium sp. strain ER1/6 isolated from Citrus sinensis

Desorption Electrospray Ionization Mass Spectrometry Imaging for Tissue Analysis

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