2016
DOI: 10.1017/s1751731116000781
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Evaluation of in situ valine production by Bacillus subtilis in young pigs

Abstract: Mutants of Bacillus subtilis can be developed to overproduce Val in vitro. It was hypothesized that addition of Bacillus subtilis mutants to pig diets can be a strategy to supply the animal with Val. The objective was to investigate the effect of Bacillus subtilis mutants on growth performance and blood amino acid (AA) concentrations when fed to piglets. Experiment 1 included 18 pigs (15.0 ± 1.1 kg) fed one of three diets containing either 0.63 or 0.69 standardized ileal digestible (SID) Val : Lys, or 0.63 SID… Show more

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“…The meal size and feeding time before blood sampling were fixed to reduce variation. Blood samples were assumed to be collected during the absorptive phase (Nørgaard et al, 2016) and were collected by jugular vein puncture into 10-mL heparinized tubes (Greiner BioOne GmbH, Kremsmünster, Austria) and placed on ice. Blood samples were centrifuged at 1,050 × g at 4 °C for 10 min, and the plasma was immediately harvested and stored at -80 °C until the laboratory analyses.…”
Section: Protocolmentioning
confidence: 99%
“…The meal size and feeding time before blood sampling were fixed to reduce variation. Blood samples were assumed to be collected during the absorptive phase (Nørgaard et al, 2016) and were collected by jugular vein puncture into 10-mL heparinized tubes (Greiner BioOne GmbH, Kremsmünster, Austria) and placed on ice. Blood samples were centrifuged at 1,050 × g at 4 °C for 10 min, and the plasma was immediately harvested and stored at -80 °C until the laboratory analyses.…”
Section: Protocolmentioning
confidence: 99%