2023
DOI: 10.1021/acs.analchem.2c03767
|View full text |Cite
|
Sign up to set email alerts
|

Evaluation of Intestinal Drug Absorption and Interaction Using Quadruple Single-Pass Intestinal Perfusion Coupled with Mass Spectrometry Imaging

Abstract: Visualization and characterization of the intestinal membrane transporter-mediated drug absorption and interaction are challenging due to the complex physical and chemical environment. In this work, an integrated strategy was developed for in situ visualization and assessment of the drug absorption and interaction in rat intestines using quadruple single-pass intestinal perfusion (Q-SPIP) technique coupled with matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI). Compared with the… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

0
3
0

Year Published

2023
2023
2024
2024

Publication Types

Select...
7

Relationship

1
6

Authors

Journals

citations
Cited by 11 publications
(3 citation statements)
references
References 47 publications
0
3
0
Order By: Relevance
“…Comprehensive metabolome analysis could contribute to active-ingredient discovery, multi-target mechanism studies and efficacy evaluation. Spatial omics based on mass spectrometry imaging with microscopy will provide new ideas for searching for active ingredients, therapeutic targets, and revealing the effective mechanisms of natural products [22][23][24]. At present, the high-throughput mass-spectrometry imaging technology is an effective analytical method that can simultaneously visualize and spatially detect, quantify, and image small metabolite molecules to understand complex communication networks.…”
Section: Advanced Mass Spectrometrymentioning
confidence: 99%
“…Comprehensive metabolome analysis could contribute to active-ingredient discovery, multi-target mechanism studies and efficacy evaluation. Spatial omics based on mass spectrometry imaging with microscopy will provide new ideas for searching for active ingredients, therapeutic targets, and revealing the effective mechanisms of natural products [22][23][24]. At present, the high-throughput mass-spectrometry imaging technology is an effective analytical method that can simultaneously visualize and spatially detect, quantify, and image small metabolite molecules to understand complex communication networks.…”
Section: Advanced Mass Spectrometrymentioning
confidence: 99%
“…Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) is a powerful tool for in situ chemical profiling from complex tissues due to its high throughput and sensitivity. However, accurately determining the CC location of lipid isomers remains challenging, despite significant advancements in MALDI MSI in spatial lipidomics research . Conventional tandem MS with low-energy collision-induced dissociation (CID) fails to locate CC due to the higher collision energy required to break the CC bond.…”
Section: Introductionmentioning
confidence: 99%
“…(CC-SF) is a common herbal couple in many traditional Chinese medicines applied to various diseases, including diarrhea, metabolic disorders, and colorectal cancer [1,2]. In recent years, there are extensive studies on CC-SF in treating ulcerative colitis (UC) [3,4]. UC is a chronic and recurrent disease characterized by loss of weight, diarrhea, and bloody stool.…”
Section: Introductionmentioning
confidence: 99%