Evaluation of Intracameral Injection of Ranibizumab and Bevacizumab on the Corneal Endothelium by Scanning Electron Microscopy

Arı, Şeyhmus; Nergız, Yusuf; Akşit, İhsan; Şahin, Alparslan; Cingü, Kürşat; Çaça, İhsan

doi:10.1089/jop.2014.0005

Cited by 12 publications

(13 citation statements)

References 27 publications

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“…Fakat, literatürde bildirilen çalışmalarda, intravitreal ranibizumab, bevacizumab ve pegaptanib uygulamalarının endotel üzerine toksik etkilerinin olmadığı gösterilmiştir. 8,9 Mevcut literatürde, diyabetli hastalarda makuler ödem tedavisi için uygulanan intravitreal aflibercept enjeksiyonunun kornea endoteline ve ön kamaraya etkisini değerlendiren bir çalışma yoktur.…”

Section: Discussionunclassified

“…tarafından, daha yüksek ön kamara konsantrasyonlarının oluştuğu ön kamaraya uygulanan anti-VEBF enjeksiyonlarında dahi tavşan kornea endotelinde toksik etkinin olmadığı bildirilmiştir. 9 VEBF'nin ve reseptörlerinin kornea gibi avasküler bir yapının fizyolojisinde nasıl bir rol oynadığı tam olarak aydınlatılamamıştır. Ambati ve ark., korneada bulunan VEBF'nin neredeyse tamamının sflt-1 isimli bir protein tarafından inaktive edildiğini ve sadece korneal yaralanma ya da inflamasyon durumunda VEBF'nin aktif forma dönebildiğini bildirmişlerdir.…”

Section: Discussionunclassified

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The Effect of Intravitreal Injection of Aflibercept and Ranibizumab on Cornea Endothelium and Anterior Chamber in Patients with Diabetic Macular Edema

Cankurtaran¹,

Özateş²

2020

Turkiye Klinikleri J Ophthalmol

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Vasküler endotelyal büyüme faktörü (VEBF), dokulardaki anjiyogenezi düzenlemektedir ve diyabetik retinopati gibi göz hastalıklarının patogene-zinde temel bir rol oynamaktadır. 1,2 VEBF neovaskülarizasyonu ve endotelyal geçirgenliği artırıcı etkileri nedeni ile göz hastalıklarının tedavisinde en çok ÖZET Amaç: İntravitreal ranibizumab ve aflibercept enjeksiyonu yapılan gözlerdeki kornea endotel sayısı ve morfolojisinin ve ön kamara anatomisinin incelenmesidir. Gereç ve Yöntemler: Bu prospektif ve kesitsel çalışmaya, diyabetik makuler ödem nedeni ile tedavi edilen 51 hastanın 102 gözü dâhil edildi. Hastaların tek gözüne 1 ay ara ile üç doz intravitreal ranibizumab ya da aflibercept enjeksiyonu uygulandı. Ranibizumab enjeksiyonu yapılan hastalar ranibizumab grubuna (n=26), aflibercept enjeksiyonu yapılan hastalar aflibercept grubuna (n=25) dâhil edildi. Her iki grupta da hastaların tedavi edilmeyen gözleri kontrol grubu olarak alındı. Hastaların endotel hücre sayısı (EHS), hücre alanı değişim katsayısı (CV), altıgen şekilli hücre (HKZ) yüzdesi, merkezi kornea kalınlığı (MKK), ön kamara derinliği (ÖKD), ön kamara hacmi (ÖKH), iridokorneal açı (İKA) ve horizontal ön kamara çapı (HÖKÇ) değerlendirmeleri yapıldı. Bulgular: Aflibercept ve ranibizumab grubunda hem tedavi edilen gözlerde hem de kontrol gözlerde tedavi öncesi ve sonrasında ortalama korneal endotel hücre sayısı, CV ve HKZ yüzdesi bakımından istatistiksel olarak anlamlı fark yoktu. Her iki gruptaki ortalama MKK değeri hem tedavi edilen gözlerde hem de kontrol gözlerde tedavi öncesinde tedavi sonrasına göre anlamlı olarak daha fazla idi (p<0,05). Her iki grupta, tedavi edilen ve kontrol gözlerde ortalama ÖKD, ÖKH, İKA ve HÖKÇ değerleri arasında tedavi öncesi ve sonrası anlamlı bir fark izlenmedi. Sonuç: Çalışmamız, intravitreal aflibercept ve ranibizumab enjeksiyonunun kornea endotelinde ve ön kamara yapısında herhangi bir değişikliğe sebep olmadığını göstermiştir. Anah tar Ke li me ler: Aflibercept; ranibizumab; makuler ödem;kornea; endotel ABS TRACT Objective: To assess the effect of intravitreal injection of aflibercept and ranibizumab on cornea endothelium and anterior chamber structure in patients with diabetic macular edema. Material and Methods: One hundred and two eyes of the 51 patients with diabetic macular edema were included in this prospective and cross-sectional clinical study. Intravitreal aflibercept or ranibizumab injection was administered for three times with one-month intervals to only one eye of the patients. Patients formed ranibizumab group (n=26) and aflibercept group (n=25) according to administered drug. Contralateral eyes, which were not treated, in both groups formed the control group. Main outcomes were endothelial cell density (ECD), coefficient of variation of cell area (CV), percentage of hexagonal cells (HEX), central corneal thickness (CCT), anterior chamber depth (ACD), anterior chamber volume (ACV), iridocorneal angle (ICA), horizontal anterior chamber diameter (HACD). Results: Mean ECD, CV and HEX before and after treatment...

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Section: Discussionunclassified

The Effect of Intravitreal Injection of Aflibercept and Ranibizumab on Cornea Endothelium and Anterior Chamber in Patients with Diabetic Macular Edema

Cankurtaran¹,

Özateş²

2020

Turkiye Klinikleri J Ophthalmol

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“…Burighel, Caicci, and Manni () reported similar structures in scanning electron micrographs of the coronal organ of an adult ascidian to be apical extensions of the membranes of supporting cells. A number of other studies have included micrographs that show similar structures on the ascidian coronal organ (Caicci, Burighel, & Manni, ; Caicci et al, ; Manni, Mackie, Caicci, Zaniolo, & Burighel, ) and on the cornea of rabbits (Ari et al, ). Examination by transmission electron microscopy is needed to reveal the relationship of these structures in urns to the junctional complex of urns reported by Jans and Jangoux ().…”

Section: Discussionmentioning

confidence: 99%

Development and morphology of ciliary urns in the sea cucumber Synaptula hydriformis (Echinodermata: Holothuroidea)

Curtis

Turner

2019

Invertebrate Biology

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Sea cucumbers (holothuroids) lack the only known echinoderm immune organ, the axial organ. Holothuroids of the families Synaptidae and Chiridotidae have coelomic organs, known as ciliary urns, that gather and excrete waste and, therefore, might function in immunity. Although ciliary urns are widely reported and illustrated in the literature, the process and histology of urn development remain unknown. Development and structure of ciliary urns were examined in Synaptula hydriformis using scanning electron, brightfield, and scanning laser confocal microscopy. Mature urns occurred on all three mesenteries in 10‐tentacled young and later growth stages, and developing urns were found in post‐pentactulae, 10‐tentacled young, and released juveniles. Developing urns were circular clusters of ciliated collar cells protruding from the mesentery. The cells increased in number to form the sessile cushion stage with a shallow lumen. The subsequent spoon‐shaped stage had a stalk and a deepened lumen with an extensive ciliary field where coelomocytes began to accumulate. Mature urns had a thin stalk and cornucopia‐shaped body with an abluminal epithelium of squamous cells and an adluminal epithelium of densely packed ciliated collar cells. Cell boundaries of the rim of mature urns and of the stalk and body of developing urns were outlined on one or both sides by microvilli and an elevated cell membrane. Ciliary urns resembling the cushion‐stage urns of S. hydriformis have been described in the sea star Archaster typicus. If urns in these groups are homologous, it is likely that cushion urns are plesiomorphic and that they are present and have been overlooked in other echinoderms.

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“…Rabbits have been widely used as an experimental model for ophthalmic research (ATILLA et al, 2003;MENCUCCI et al, 2005;ARI et al, 2015). The goal of the present study was to evaluate the cell density and percentage of hexagonal cells because these are the most reliable parameters for evaluating the integrity of the corneal endothelium (ABIB & BARRETO, 2001;PIGATTO et al, 2006;NAGATSUYU et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that rabbit corneal endothelium repairs by cell division and Brambatti et al migration (MORITA, 1995). Rabbits have been widely employed in the evaluation of the effects of surgical procedures and drugs on the corneal endothelium (ATILLA et al, 2003;MENCUCCI et al, 2005;ARI et al, 2015). However, there are few published references regarding endothelial density in rabbits.…”

Section: Introductionmentioning

confidence: 99%

Corneal endothelial cell density and morphology in rabbits’ eyes using contact specular microscopy

et al. 2017

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The goal of this study was to describe the normal values of corneal endothelial cell density and hexagonality in healthy rabbits’ eyes using contact specular microscopy. Both eyes of 18 rabbits, males and females, of different ages were evaluated. The eyes were divided into three groups of 12 each according to the age range of the animals: G1 (6 months old), G2 (12 months old), and G3 (48 months old). Parameters studied included mean endothelial cell density and hexagonality. For Group I, the mean cell density was 2307 cells per mm2. For GII, the mean cell density was 1895 cells per mm2. For G III, the mean cell density was 1818 cells per mm2. Cell density decreased significantly with aging (P<0.001) among the three groups. Pleomorphism in the rabbits of GI was 74.33±10.08. In the rabbits of GII it was 71.83±11.38. In the rabbits of GIII it was 64.02±28.80. Significant differences (P<0.001) were evident between GI and GIII and GII and GIII but not between Groups I and II. The results showed a decrease in number of cells and hexagonality with aging. Corneal endothelium of rabbits suffers changes due to advancing age both in terms of cell density and morphology.

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Evaluation of Intracameral Injection of Ranibizumab and Bevacizumab on the Corneal Endothelium by Scanning Electron Microscopy

Cited by 12 publications

References 27 publications

The Effect of Intravitreal Injection of Aflibercept and Ranibizumab on Cornea Endothelium and Anterior Chamber in Patients with Diabetic Macular Edema

The Effect of Intravitreal Injection of Aflibercept and Ranibizumab on Cornea Endothelium and Anterior Chamber in Patients with Diabetic Macular Edema

Development and morphology of ciliary urns in the sea cucumber Synaptula hydriformis (Echinodermata: Holothuroidea)

Corneal endothelial cell density and morphology in rabbits’ eyes using contact specular microscopy

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