Evaluation of laser speckle contrast imaging as an intrinsic method to monitor blood brain barrier integrity

Dufour, Suzie; Atchia, Yaaseen; Gad, Raanan; Ringuette, Dene; Sigal, Iliya; Levi, Ofer

doi:10.1364/boe.4.001856

Cited by 15 publications

(12 citation statements)

References 54 publications

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“…Another possible mechanism is that the increased vessel strain during larger SLEs results in an increase in plasma (or simply water) crossing the vascular epithelium. This is similar to the mismatch in arterial versus venous flow which we previously demonstrated using LSCI in a model of blood-brain barrier (BBB) leakage [23]. The results from LSCI-ECG synchronization presented here suggest an opportunity to measure more subtle changes in BBB permeability.…”

Section: Discussionsupporting

confidence: 86%

Continuous multi-modality brain imaging reveals modified neurovascular seizure response after intervention

Ringuette

Jeffrey

Dufour³

et al. 2017

Biomed. Opt. Express

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We developed a multi-modal brain imaging system to investigate the relationship between blood flow, blood oxygenation/volume, intracellular calcium and electrographic activity during acute seizure-like events (SLEs), both before and after pharmacological intervention. Rising blood volume was highly specific to SLE-onset whereas blood flow was more correlated with all eletrographic activity. Intracellular calcium spiked between SLEs and at SLE-onset with oscillation during SLEs. Modified neurovascular and ionic SLE responses were observed after intervention and the interval between SLEs became shorter and more inconsistent. Comparison of artery and vein pulsatile flow suggest proximal interference and greater vascular leakage prior to intervention.

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Section: Discussionsupporting

confidence: 86%

Continuous multi-modality brain imaging reveals modified neurovascular seizure response after intervention

Ringuette

Jeffrey

Dufour³

et al. 2017

Biomed. Opt. Express

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“…Among in vivo monitoring methods, magnetic resonance imaging (MRI) and computed tomography (CT) scanners are employed for monitoring the BBB permeability . However, these techniques suffer from a low resolution and sensitivity; in addition, they are very expensive to operate and not optimized for prolonged measurement periods . In contrast, various optical imaging techniques, such as contrast‐enhanced photoacoustic micro‐imaging , laser speckle contrast imaging and fluorescent imaging , have been developed for visualization of the BBB permeability in preclinical studies on rodents.…”

Section: Introductionmentioning

confidence: 99%

“…However, these techniques suffer from a low resolution and sensitivity; in addition, they are very expensive to operate and not optimized for prolonged measurement periods . In contrast, various optical imaging techniques, such as contrast‐enhanced photoacoustic micro‐imaging , laser speckle contrast imaging and fluorescent imaging , have been developed for visualization of the BBB permeability in preclinical studies on rodents. However, the high scattering of the intact skull limits optical imaging performance (eg, imaging depth, contrast and resolution).…”

Section: Introductionmentioning

confidence: 99%

In vivo monitoring blood‐brain barrier permeability using spectral imaging through optical clearing skull window

Feng

Zhang

et al. 2018

Journal of Biophotonics

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The blood‐brain barrier (BBB) plays a key role in the health of the central nervous system. Opening the BBB is very important for drug delivery to brain tissues to enhance the therapeutic effect on brain diseases. It is necessary to in vivo monitor the BBB permeability for assessing drug release with high resolution; however, an effective method is lacking. In this work, we developed a new method that combined spectral imaging with an optical clearing skull window to in vivo dynamically monitor BBB opening caused by 5‐aminolevulinic acid (5‐ALA)‐mediated photodynamic therapy (PDT), in which the Evans blue dye (EBd) acted as an indicator of the BBB permeability. Using this method, we effectively monitored the cerebrovascular EBd leakage process. Moreover, the analysis of changes in the vascular and extravascular EBd concentrations demonstrated that the PDT‐induced BBB opening exhibited spatiotemporal differences in the cortex. This spectral imaging method based on the optical clearing skull window provides a low‐cost and simply operated tool for in vivo monitoring BBB opening process. This has a high potential for the visualization of drug delivery to the central nervous system. Thus, it is of tremendous significance in brain disease therapy. Monitoring the changes in PDT‐induced BBB permeability by evaluating the EBd concentration using an optical clearing skull window. (A) Entire brains and coronal sections following treatment of PDT with/without an optical clearing skull window after injection of EBd. (B) Typical EBd distribution maps before and after laser irradiation captured by the spectral imaging method. (Colorbar represents the EBd concentration).

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“…Quantification of motion is achieved by mathematically analyzing the spatial blurring [27,28]. A recent study [29] employed laser speckle contrast imaging to assess BBB permeability, constituting the first demonstration of LSCI in that aspect. The basis for this modality is that BBB disruption can alter cerebral blood flow [23].…”

Section: In Vivo Imaging and Assessment Of Brain Vessels' Permeabilitymentioning

confidence: 99%

Analyzing the blood–brain barrier: The benefits of medical imaging in research and clinical practice

Chassidim

Vazana

Prager

et al. 2015

Seminars in Cell & Developmental Biology

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Evaluation of laser speckle contrast imaging as an intrinsic method to monitor blood brain barrier integrity

Cited by 15 publications

References 54 publications

Continuous multi-modality brain imaging reveals modified neurovascular seizure response after intervention

Continuous multi-modality brain imaging reveals modified neurovascular seizure response after intervention

In vivo monitoring blood‐brain barrier permeability using spectral imaging through optical clearing skull window

Analyzing the blood–brain barrier: The benefits of medical imaging in research and clinical practice

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