Evaluation of live gumboro vaccine prepared from local variant strain for control of infectious bursal disease in Egypt

Abstract

Aim: The present study was designed to evaluate live Gumboro (IBD) vaccine prepared from local variant isolated strain (Egy-IBD Var 2009 Vp2 gene-, partial cds submitted in gen bank at accession no. : JN 118617) for controlling IBD problem in Egypt. Material and Methods: Local isolated variant strain was adapted on Baby Grivet Monkey kidney cell line -70(BGM-70) 7.5 4.5 used for preparation of tissue culture (T.C) live vaccine. T.C IBDV had a titer of 10 TCID /ml (10 TCID per dose) after 50 50five passages in BGM-70 cell culture. Evaluation of prepared vaccine was done in vitro by measuring ELISA, and in vivo by protection % against very virulent or variant field IBD isolated strains.Result: Evaluation revealed that the prepared vaccine was safe; sterile; pure; non-immunosuppressive; and efficient. The Geometric Mean Titer (GMT) of ELISA for the prepared vaccine was 8271 and more than 10000 in compared with different commercial IBD vaccines; while protection percentage gave 96-100%; 92-96% and 96-98% in groups vaccinated with commercial (intermediate; intermediate plus and classical) IBD vaccines; respectively in compared to 96-100% in group vaccinated with local prepared vaccine when challenged with very virulent or variant IBD isolated strains. Conclusion:We can use live T.C. IBD vaccine prepared from local variant isolated virus strain as method for control IBD disease in Egypt.

Isolation of Infectious Bursal Disease Virus Using Indigenous Chicken Embryos in Kenya

“…A study done in Bangladesh to compare isolation of IBDV using commercial hybrid chicken embryos and indigenous chicken embryos [ 15 ] showed that rural indigenous chicken eggs were better for virus isolation than commercial farm hybrid chicken eggs. Strains of IBDV circulating in North Africa [ 16 , 17 ] and West Africa [ 18 ] have been isolated for characterization and vaccine development but not in East Africa. Currently, there is no report available on isolation of local IBDV strains using embryonated indigenous chicken eggs in Kenya.…”