In the present work, a novel lubricant has been extracted from mango (Mangifera indica L.) seeds using the soxhlet technique. Lubricants from agricultural waste can reduce the shortage of edible oils and eliminates the need to dispose them. Therefore, non-edible oils are economical comparable with edible oils. In order to validate the effectiveness of extracted lubricant, thermophysical characterization and tribology tests were performed. The obtained results were compared with edible and non-edible oils, such as sunflower oil (SFO) and mahua seed oil (MaSO) respectively. Friction tests were conducted using tribometer with pin-on-disk (WC-Co/Ti-3Al-2.5V) at different tribological conditions. Worn surface and wear mechanism of disks were examined using a scanning electron microscope (SEM) associated with energy-dispersive spectroscopy (EDS) in order to determine the quantitative comparison of elemental composition. These findings showed that mango seed oil (MSO) exhibited lowest coefficient of friction and wear volume followed by dry, SFO, and MaSO respectively. SEM analysis showed that no plowing was observed, however small pits and cracks were found on wear track under MSO.