Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections

Banar, Maryam; Emaneini, Mohammad; Satarzadeh, Mhboubeh; Abdellahi, Nafiseh; Beigverdi, Reza; Leeuwen, Willem B. van; Jabalameli, Fereshteh

doi:10.1371/journal.pone.0164622

Cited by 75 publications

(92 citation statements)

References 28 publications

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“…Data are representative of two independent experiments with 5 mice per group units of D-mannose, L-rhamnose, and D-glucose, where the individual -D-mannose residues are connected with linkages, suitable for digest by the identified via LC-MS/MS -mannosidases (e.g., Man2b1), we tested the importance of -mannosidases in regulating biofilm formation and bactericidal properties of neutrophils. Consistentwith recently published data by others,44 we observed that treatment with -mannosidase in vitro decreased P. aeruginosa biofilms.…”

supporting

confidence: 93%

Frontline Science: Employing enzymatic treatment options for management of ocular biofilm-based infections

Kugadas

Geddes‐McAlister

Guy

et al. 2019

Journal of Leukocyte Biology

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Pseudomonas aeruginosa ‐induced corneal keratitis is a sight‐threatening disease. The rise of antibiotic resistance among P. aeruginosa keratitis isolates makes treatment of this disease challenging, emphasizing the need for alternative therapeutic modalities. By comparing the responses to P. aeruginosa infection between an outbred mouse strain (Swiss Webster, SW) and a susceptible mouse strain (C57BL6/N), we found that the inherent neutrophil‐killing abilities of these strains correlated with their susceptibility to infection. Namely, SW‐derived neutrophils were significantly more efficient at killing P. aeruginosa in vitro than C57BL6/N‐derived neutrophils. To interrogate whether the distinct neutrophil killing capacities were dependent on endogenous or exogenous factors, neutrophil progenitor cell lines were generated. The in vitro differentiated neutrophils from either SW or C57BL6/N progenitors retained the differential killing abilities, illustrating that endogenous factors conferred resistance. Consistently, quantitative LC‐MS/MS analysis revealed strain‐specific and infection‐induced alterations of neutrophil proteomes. Among the distinctly elevated proteins in the SW‐derived proteomes were α‐mannosidases, potentially associated with protection. Inhibition of α‐mannosidases reduced neutrophil bactericidal functions in vitro. Conversely, topical application of α‐mannosidases reduced bacterial biofilms and burden of infected corneas. Cumulatively, these data suggest novel therapeutic approaches to control bacterial biofilm assembly and improve bacterial clearance via enzymatic treatments.

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supporting

confidence: 93%

Frontline Science: Employing enzymatic treatment options for management of ocular biofilm-based infections

Kugadas

Geddes‐McAlister

Guy

et al. 2019

Journal of Leukocyte Biology

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“…Cellulase has been used to inhibit biofilm formation by P. aeruginosa and S. aureus (Fleming et al, 2017). α-Mannosidase, β-mannosidase, PelA h , and PslG H have been found to have the same effect when used against P. aeruginosa (Baker et al, 2016, Banar et al, 2016. However, the application of AlsL, cellulase, α-mannosidase, β-mannosidase, PelA h , and PslG H against foodassociated bacteria is still limited.…”

Section: Alginate Lyasementioning

confidence: 99%

Advances and Future Prospects of Enzyme‐Based Biofilm Prevention Approaches in the Food Industry

Begum

Mizan

et al. 2018

Comp Rev Food Sci Food Safe

114

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Food poisoning and foodborne diseases are a growing public health concern worldwide. Approximately 30 known and many unknown pathogens are the main culprits for these conditions. Biofilms are a heterogeneous living‐form of pathogens and are considered a safe haven for their pathogenicity. In the field of food processing, the persistence of biofilms results in an increased likelihood of food contamination, which ultimately compromises overall food quality and safety. Because of the robust heterogeneity and resistant phenotypic nature of biofilms, the impairment of biofilms is very challenging when using conventional cleaning agents/antibiotics. Therefore, the development of alternative approaches is of great interest to the food industry. Recently, many researchers have found that use of enzymes can provide an exciting and effective therapeutic approach for solving biofilm‐associated problems in the food industry, because enzymes are involved in almost every stage of biofilm detachment and degradation. Here, we describe biofilm‐associated problems in the food industry and recent advances in enzyme‐based biofilm impairment strategies. We also highlight major limitations, challenges, and possible prospects of enzyme‐based biofilm‐targeting technologies.

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“…Therefore, the use of novel therapies that specifically disrupt biofilms within chronic wounds is a promising strategy for wound-care therapies. In this context, matrix-degrading enzymes have received particular attention and been used for the degradation of biofilm EPS [10][11][12][13][14][15]. However, the efficiency of each EPS-degrading enzyme will depend on the EPS composition [16,17].…”

Section: Introductionmentioning

confidence: 99%

“…Trypsin is a serine endoprotease that cleaves proteins or peptides and may depolymerize protein contents of bacterial biofilms. It has previously been shown that these two enzymes could disrupt in vitro mono-species biofilms of P. aeruginosa, S. aureus, Streptococcus pneumonia, and Staphylococcus epidermidis [10,15,16,29,30].…”

Section: Introductionmentioning

confidence: 99%

Combinatorial effects of antibiotics and enzymes against dual-species Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the wound-like medium

et al. 2020

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Bacterial biofilms are one of the major issues in the treatment of chronic infections such as chronic wounds, where biofilms are typically polymicrobial. The synergy between species can occur during most polymicrobial infections, where antimicrobial resistance enhances as a result. Furthermore, self-produced extracellular polymeric substance (EPS) in biofilms results in a high tolerance to antibiotics that complicates wound healing. Since most antibiotics fail to remove biofilms in chronic infections, new therapeutic modalities may be required. Disruption of EPS is one of the effective approaches for biofilm eradication. Therefore, degradation of EPS using enzymes may result in improved chronic wounds healing. In the current study, we investigated the efficacy of trypsin, β-glucosidase, and DNase I enzymes on the degradation of dual-species biofilms of Pseudomonas aeruginosa and Staphylococcus aureus in a wound-like medium. These species are the two most common bacteria associated with biofilm formation in chronic wounds. Moreover, the reduction of minimum biofilm eradication concentration (MBEC) of meropenem and amikacin was evaluated when combined with enzymes. The minimum effective concentrations of trypsin, β-glucosidase, and DNase I enzymes to degrade biofilms were 1 μg/ml, 8 U/ml, and 150 U/ml, respectively. Combination of 0.15 μg/ml trypsin and 50 U/ml DNase I had a significant effect on S. aureus-P. aeruginosa biofilms which resulted in the dispersal and dissolution of all biofilms. In the presence of the enzymatic mixture, MBECs of antibiotics showed a significant decrease (p < 0.05), at least 2.5 fold. We found that trypsin/DNase I mixture can be used as an anti-biofilm agent against dual-species biofilms of S. aureus-P. aeruginosa.

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Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections

Cited by 75 publications

References 28 publications

Frontline Science: Employing enzymatic treatment options for management of ocular biofilm-based infections

Frontline Science: Employing enzymatic treatment options for management of ocular biofilm-based infections

Advances and Future Prospects of Enzyme‐Based Biofilm Prevention Approaches in the Food Industry

Combinatorial effects of antibiotics and enzymes against dual-species Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the wound-like medium

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