Evaluation of Media and Reagents for Indole-spot Tests in Anaerobic Bacteriology

Sutter, Vera L.; Carter, Walker T.

doi:10.1093/ajcp/58.3.335

Cited by 38 publications

(15 citation statements)

References 2 publications

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“…In looking down the columns of Table 3, it is apparent that individual negative carbohydrate results were recorded more often with the CONV (66) and PRAS (62) than with the MICRO system (38). Although individual positive carbohydrate results were much higher with PRAS (191) than with CONV (19) or MICRO (12), this was largely a result of recording of weak positive results (pH 5.6 to 5.9) in the PRAS systems which were recorded as weak, variable, or negative identification schemes. Total agreements between pairs of procedures were counted so that the direct comparisons given in Table 4 could be made.…”

Section: Resultsmentioning

confidence: 94%

Comparison of three procedures for biochemical testing of anaerobic bacteria

Moore¹,

Sutter²,

Finegold³

1975

J Clin Microbiol

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The Analytab Products, Inc. (API), anaerobic multitest microsystem (MI-CRO) was compared with the Center for Disease Control conventional (CONV) thioglycolate (supplemented with hemin and vitamin Kl) system and with pre-reduced anaerobically sterilized (PRAS) media as recommended by the Virginia Polytechnic Institute. Growth from a solid medium was suspended to produce standard inocula. Substrates included 16 carbohydrates, indole, urea, gelatin, and esculin. API strips were inoculated in air and incubated in GasPak (BBL) jars. MICRO tests were read at 1 and 2 days, CONV tests at 1, 2, and 7 days, and PRAS tests at 3 weeks. One hundred thirty well-characterized strains of anaerobes (76 gram-negative rods, 16 cocci, 26 gram-positive nonsporeforming rods, and 12 clostridia), including 48 reference strains, were studied. Of 2,600 tests performed, 2,085 (80.2%) showed agreement with all three methods. There was 90.9% agreement between the MICRO and CONV, 84.9% between the MICRO and PRAS, and 84.6% between the CONV and PRAS tests. All MICRO tests were reliable except for indole, which was not sensitive enough, and gelatin, which was very insensitive. The MICRO system permits performance of biochemical tests at the workbench in the average clinical laboratory without the need for expensive equipment and time-consuming procedures.

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Section: Resultsmentioning

confidence: 94%

Comparison of three procedures for biochemical testing of anaerobic bacteria

Moore¹,

Sutter²,

Finegold³

1975

J Clin Microbiol

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“…Kaufman and Weaver (8) also used several species of Clostridium in their report of a combined media for detection of gelatin hydrolysis and indole production. In 1972, Sutter and Carter (15) evaluated four indole-spot tests for use in anaerobic bacteriology.…”

Section: Discussionmentioning

confidence: 99%

Rapid Methods for Biochemical Testing of Anaerobic Bacteria

Schreckenberger¹,

Blazevic²

1974

Applied Microbiology

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Rapid biochemical tests for nitrate, indole, gelatin, starch, esculin, and o-nitrophenyl-,B-D-galactopyranoside were performed on 112 strains of anaerobic bacteria. All tests were incubated under aerobic conditions, and results were recorded within 4 h. The tests for nitrate, indole, and starch showed a 95% or greater correlation when compared to the standard biochemical tests. Tests for esculin and gelatin showed an agreement of 86 and 77%, respectively. PathoTec test strips for nitrate, indole, esculin, o-nitrophenyl-,B-D-galactopyranoside, Voges-Proskauer, and urease were also tested and showed encouraging results.

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“…Bacterial sialidase activity and the spot indole (p-dimethlyaminocinnamaldehyde method) tests were performed as described previously (9,11,19).…”

Section: Methodsmentioning

confidence: 99%

Rapid presumptive identification of black-pigmented gram-negative anaerobic bacteria by using 4-methylumbelliferone derivatives

et al. 1991

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A rapid method for presumptive identification of black-pigmented gram-negative anaerobic rods was developed. Using filter paper spot tests for indole production, sialidase, ot-glucosidase, I-glucosidase, ft-fucosidase, and trypsinlike enzyme activities, 100% of Porphyromonas gingivalis, PrevoteUa intermedia, and Bacteroides levii and 89% of Prevotella corporis isolates were correctly identified to the species level. Porphyromonas asaccharolytica and Porphyromonas endodontalis could not be differentiated from each other but could be distinguished from all other species tested. Similarly, Prevotella denticola, PrevoteUa loescheii, and PrevoteUa melaninogenica could not be differentiated from each other. The methods described are based on 4-methylumbelliferone derivatives of the various substrates and are simple to perform, rapid (<15 min), and applicable to difficult-to-cultivate anaerobic rods.

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Evaluation of Media and Reagents for Indole-spot Tests in Anaerobic Bacteriology

Cited by 38 publications

References 2 publications

Comparison of three procedures for biochemical testing of anaerobic bacteria

Comparison of three procedures for biochemical testing of anaerobic bacteria

Rapid Methods for Biochemical Testing of Anaerobic Bacteria

Rapid presumptive identification of black-pigmented gram-negative anaerobic bacteria by using 4-methylumbelliferone derivatives

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