Evaluation of Microcapsule Permeability via Inverse Size Exclusion Chromatography

Briššová, Marcela; Petro, Miroslav; Lacı́k, Igor; Powers, Alvin C.; Wang, T.

doi:10.1006/abio.1996.0435

Cited by 85 publications

(30 citation statements)

References 14 publications

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“…Polysaccharide elution chromatograms (Fig. S3 supplement) are broad, which is in agreement with previously reported results [30]. This observation is likely connected to the large sizes of the solute molecule (e.g., slower movement of large molecules in stagnant liquid phase) and is partly caused by the polydispersity of dextran and pullulan standards.…”

Section: Pore Size Distributionsupporting

confidence: 91%

“…The elution volume for each standard was determined as the volume at which 50 % of the standard was eluted. This quantity was used to determine the chromatographic partition coefficients providing the MWCO of column packing in the form of the molecular weight and viscosity radius R η as described elsewhere [30,31]. The Waters SEC setup was equipped with a Waters 2410 refractive index detector.…”

Section: Inverse Size Exclusion Chromatography (Isec)mentioning

confidence: 99%

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Physical and Bioengineering Properties of Polyvinyl Alcohol Lens-Shaped Particles Versus Spherical Polyelectrolyte Complex Microcapsules as Immobilisation Matrices for a Whole-Cell Baeyer–Villiger Monooxygenase

Schenkmayerová

Bučko

Gemeiner

et al. 2014

Appl Biochem Biotechnol

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Direct comparison of key physical and chemical-engineering properties of two representative matrices for multipurpose immobilisations was performed for the first time. Polyvinyl alcohol lens-shaped particles LentiKats® and polyelectrolyte complex microcapsules were characterised by advanced techniques with respect to the size distribution of the particles, their inner morphology as revealed by fluorescent probe staining, mechanical resistance, size-exclusion properties, determination of effective diffusion coefficient and environmental scanning electron microscope imaging. While spherical polyelectrolyte complex microcapsules composed of a rigid semipermeable membrane and a liquid core are almost uniform in shape and size (diameter of 0.82 mm; RSD = 5.6 %), lens-shaped LentiKats® are characterised by wider size distribution (diameter of 3.65 mm; RSD = 10.3 % and height of 0.341 mm; RSD = 32.3 %) and showed the same porous structure throughout their whole volume at the mesoscopic (micrometre) level. Despite differences in their inner structure and surface properties, the pore diameter of ∼ 2.75 nm for regular polyelectrolyte complex microcapsules and ∼ 1.89 nm for LentiKats® were similar. These results were used for mathematical modelling, which provided the estimates of the effective diffusion coefficient of sucrose. This value was 1.67 × 10(-10) m(2) s(-1) for polyelectrolyte complex microcapsules and 0.36 × 10(-10) m(2) s(-1) for LentiKats®. Recombinant cells Escherichia coli-overexpressing enzyme cyclopentanone monooxygenase were immobilised in polyelectrolyte complex microcapsules and LentiKats® for comparison of their operational stability using model Baeyer-Villiger oxidation of (±)-cis-bicyclo [3.2.0] hept-2-en-6-one to regioisomeric lactones as important chiral synthons for potential pharmaceuticals. Both immobilisation matrices rendered high operational stability for whole-cell biocatalyst with no reduction in the biooxidation rate over 18 repeated reaction cycles.

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Section: Pore Size Distributionsupporting

confidence: 91%

Section: Inverse Size Exclusion Chromatography (Isec)mentioning

confidence: 99%

Physical and Bioengineering Properties of Polyvinyl Alcohol Lens-Shaped Particles Versus Spherical Polyelectrolyte Complex Microcapsules as Immobilisation Matrices for a Whole-Cell Baeyer–Villiger Monooxygenase

Schenkmayerová

Bučko

Gemeiner

et al. 2014

Appl Biochem Biotechnol

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“…This value allows an estimation of the size of excluded pullulan standard expressed by the viscosity radius (R η ) of about 4.2 nm. 12 This value corresponds 12 to the cut-off value for excluded globular proteins of molecular weight about 116 000 Da, which is approximately in the range of the molecular weight of CES hydrolase (60 000-95 000 Da).…”

Section: Permeability Of Capsule Membranementioning

confidence: 98%

“…The molecular weight cut-off (MWCO) was determined by inverse size exclusion chromatography (SEC) 11,12 . Briefly, narrow molecular weight distribution pullulan standards (Gearing Scientific, Ashwell, Herts, UK), over the molecular weight range from 738 to 788 000 Da, were injected onto a glass column 10 × 250 mm (Omnifit, Cambridge, UK), fitted with an adjustable plunger, filled with capsules.…”

Section: Permeability Of Capsular Membranementioning

confidence: 99%

Nocardia tartaricans cells immobilized in sodium alginate–cellulose sulfate–poly (methylene‐co‐guanidine)capsules: mechanical resistance and operational stability

Bučko

Vikartovská

Gemeiner

et al. 2006

J of Chemical Tech & Biotech

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The stereospecific hydrolysis of cis-epoxysuccinate (CES) to L-(+)-tartrate, catalysed by the action of CES hydrolase activity in whole Nocardia tartaricans bacterial cells, has been chosen as a model biotransformation system. The performance of cells was studied by immobilization in polyelectrolyte complex (sodium alginate-cellulose sulfate-poly(methylene-co-guanidine, SA-CS/PMCG) capsules based on sodium alginate and cellulose sulfate as polyanions, poly(methylene-co-guanidine) as polycation, CaCl 2 as gelling agent and NaCl as antigelling agent. The encapsulated cells showed a preservation of 80% of the initial catalytic activity in encapsulated cells compared with 15% of retained catalytic activity in free cells after 10 repeated biotransformation cycles. Uniformity of batches of capsules was achieved by the use of an air-stripping extrusion device fitted with a multiloop reactor. The capsules produced using this device had narrow standard deviations for capsule diameter (<3%) and membrane thickness (<6%). Monodisperse capsules are essential to ensure constant properties, including mechanical resistance and diffusional characteristics. Storage of capsules under different conditions revealed an approximate threefold decrease in bursting force after long-term exposure to the highly sequestering CES, compared with capsules stored in 0.9% (w/v) NaCl. The molecular weight cut-off of the capsular membrane, determined by inverse size exclusion chromatography, is in the range of the size of CES hydrolase.

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“…Smidsrød and Skjåk-Braek 77 found that the pore diameters in 2% alginate range from 5 to 200 nm. Based on the work of Bri s sov a et al, 78 who studied the permeability and exclusion limits of alginate microcapsules, a 5 nm viscous radius would correspond to a globular protein such as c-globulin with molecular weight of 158 kDa, while a 200 nm viscous radius would be larger than a globular protein of >1000 kDa. Van Elk et al 79 used 3% Manucol LKX alginate, a higher concentration of the same alginate used in the work presented here, and found that fluorescein (0.7 nm, 332.31 g/mol) was able to diffuse freely through their 3% alginate matrix.…”

Section: Drug Screening Shows Multicellular Resistance Compared Tomentioning

confidence: 99%

Core-shell hydrogel beads with extracellular matrix for tumor spheroid formation

Grist

Nasseri

et al. 2015

Biomicrofluidics

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Creating multicellular tumor spheroids is critical for characterizing anticancer treatments since they may provide a better model of the tumor than conventional monolayer culture. Moreover, tumor cell interaction with the extracellular matrix can determine cell organization and behavior. In this work, a microfluidic system was used to form cell-laden core-shell beads which incorporate elements of the extracellular matrix and support the formation of multicellular spheroids. The bead core (comprising a mixture of alginate, collagen, and reconstituted basement membrane, with gelation by temperature control) and shell (comprising alginate hydrogel, with gelation by ionic crosslinking) were simultaneously formed through flow focusing using a cooled flow path into the microfluidic chip. During droplet gelation, the alginate acts as a fast-gelling shell which aids in preventing droplet coalescence and in maintaining spherical droplet geometry during the slower gelation of the collagen and reconstituted basement membrane components as the beads warm up. After droplet gelation, the encapsulated MCF-7 cells proliferated to form uniform spheroids when the beads contained all three components: alginate, collagen, and reconstituted basement membrane. The dose-dependent response of the MCF-7 cell tumor spheroids to two anticancer drugs, docetaxel and tamoxifen, was compared to conventional monolayer culture.

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Evaluation of Microcapsule Permeability via Inverse Size Exclusion Chromatography

Cited by 85 publications

References 14 publications

Physical and Bioengineering Properties of Polyvinyl Alcohol Lens-Shaped Particles Versus Spherical Polyelectrolyte Complex Microcapsules as Immobilisation Matrices for a Whole-Cell Baeyer–Villiger Monooxygenase

Physical and Bioengineering Properties of Polyvinyl Alcohol Lens-Shaped Particles Versus Spherical Polyelectrolyte Complex Microcapsules as Immobilisation Matrices for a Whole-Cell Baeyer–Villiger Monooxygenase

Nocardia tartaricans cells immobilized in sodium alginate–cellulose sulfate–poly (methylene‐co‐guanidine)capsules: mechanical resistance and operational stability

Core-shell hydrogel beads with extracellular matrix for tumor spheroid formation

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