Evaluation of Microleakage of Silver Nanoparticle-Added Pit and Fissure Sealant in Permanent Teeth (In-Vitro Study)

Abstract

INTRODUCTION: Silver has long been recognized for its antimicrobial effect on a wide spectrum of microorganisms. Consequently, silver nanoparticles became among the most common therapeutic nanoparticles under research in the field of caries prevention. Pit-and-fissure sealants are among the major components in the field of caries prevention which could have an additional antibacterial benefit through silver nanoparticles addition. OBJECTIVES:The aim of the study was to evaluate, in-vitro, the marginal seal of silver nanoparticle-added pits and fissure sealant in comparison to the conventional sealant in permanent teeth. MATERIALS AND METHODS: Thirty sound premolar teeth extracted for orthodontic purposes were randomly assigned into two main groups; Group I (n=15) for silver nanoparticle-added ClinproTM sealant and Group II (n=15) for the conventional ClinproTM sealant. Silver nanoparticle-added sealant was prepared by mixing the contents of one syringe of ClinproTM sealant to silver nanopowder of particle size 50-80nm at a concentration of 6.25μg/ml. For microleakage testing, after sealant application and thermocycling for 500 thermal cycles, the sealed teeth was immersed into 1% methylene blue solution for 24h, sectioned longitudinally in a bucco-lingual direction and then examined under stereomicroscope for dye penetration assessment at x60 magnification. RESULTS: Using Mann Whitney U test there was no statistically significant difference in microleakage between both groups (P= 0.325) CONCLUSION: Addition of silver nanoparticles to pit-and-fissure sealant did not affect its sealing ability.

The Effect of Enamel Pre-treatment with Silver Diamine Fluoride versus Nano Silver Fluoride on the Microleakage of Fissure Sealant: in vitro study.

“…Our finding that fissure pre-treatment with NSF didn't affect micro-leakage scores of the FS was supported by Morales et al, (34) and Khairy et al (35) who concluded that that there was no significant difference in the marginal seal or micro-leakage between the silver nanoparticle-containing sealant and the conventional sealant when applied in primary molars or permanent premolars respectively.…”

Evaluation of Remineralizing Ability and Mechanical Properties of Two Fluoride Rechargeable Fissure Sealants in Primary Molars: An In vitro Study

“…Duration of exposure at each temperature was 20 seconds. (26) After thermocycling, all surfaces of (16) molars were coated with two layers of nail varnish except for 1.5mm from the sealant margins. Then immersed in a 1% solution of methylene blue for 24 hrs, rinsed with distilled water, dried, and sectioned longitudinally in a bucco-lingual direction using a water-cooled diamond disk.…”

Nanotechnology in Pit and Fissure Sealants: A Systematic Review

“…Another possible limitation would be that extracted teeth do not have the pulp pressure and intratubular fl uid pressure as in natural teeth. This could have an impact on tooth moisture level, aff ecting microleakage at tooth restoration interface 34 . While in another study, the addition of silver NPs in sealants reduced tooth demineralization signifi cantly.…”