Evaluation of minimally invasive muscle biopsy method for genetic analysis in horse

Stefaniuk‐Szmukier, Monika; Ropka‐Molik, Katarzyna; Piórkowska, Katarzyna; Bereta, Anna; Szpar, Paweł; Czerwonka, Zbigniew; Podstawski, Zenon

doi:10.1515/aoas-2015-0017

Cited by 5 publications

(4 citation statements)

References 16 publications

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“…The whole blood samples were obtained by way of jugular venipuncture and collected into RNAlater tubes (Applied Biosystems, Foster City, CA, USA; ThermoFisher Scientific, Waltham, MA, USA), whereas muscle samples were collected by way of a biopsy method in accordance with procedures previously described by our team [20]. These were then stored at −80 °C.…”

Section: Methodsmentioning

confidence: 99%

The Blood and Muscle Expression Pattern of the Equine TCAP Gene during the Race Track Training of Arabian Horses

Stefaniuk‐Szmukier

Szmatoła

Łątka

et al. 2019

Animals

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Horse musculature has been shaped through evolution by environmental and human factors, which has resulted in several extraordinary adaptations to physical effort. Skeletal muscle plasticity results from the response to mechanical stimulation causing hypertrophy, where sarcomeres increase the muscle’s cross-sectional area under the influence of contractile forces. The aim of the present study was the evaluation of transcript abundance of the telethonin (TCAP) gene, which is a part of the sarcomere macromolecular mechanosensory complex in the gluteus medius muscle, and the whole blood of Arabian horses during flat race training. The analysis, performed by quantitative PCR, showed an increase of TCAP transcripts in skeletal muscle. However, in whole blood, the transcript abundance decreased after the first stage of training and further increased after the second phase. The obtained results indicate a lack of similarity of TCAP gene expression in both tissues.

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Section: Methodsmentioning

confidence: 99%

The Blood and Muscle Expression Pattern of the Equine TCAP Gene during the Race Track Training of Arabian Horses

Stefaniuk‐Szmukier

Szmatoła

Łątka

et al. 2019

Animals

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“…The exact training procedure and points of muscle collection were described previously [ 10 ]. Muscle biopsy was performed using a ProMag™ Ultra Automatic Biopsy Instrument (Argon Medical Devices, Inc., Creek Road, USA) with a 2-mm-diameter biopsy needle according to the procedure described by Stefaniuk et al [ 14 ]. The experimental protocol was approved by the Animal Care and Use Committee of the Institute of Pharmacology, Polish Academy of Sciences in Cracow (no.…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of the apoptosis-related SH3RF1 and SH3RF2 genes and their association with exercise performance in Arabian horses

et al. 2018

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BackgroundApoptosis plays an important role in the regulation of healthy tissue growth and development as well as in controlling the maintenance of homeostasis in exercising muscles. During an intensive physical effort, the regulation of cell death by apoptosis results in the replacement of unaccustomed muscle cells by new cells that are better suited to exercise. The aim of this study was to determine the expression of two genes (SH3FR1 and SH3RF2) that control apoptosis in muscle tissues during training periods characterized by different intensities. The gene expression levels were estimated using real-time PCR method in skeletal muscle biopsies collected from 15 Arabian horses (untrained, after an intense gallop phase, and at the end of the racing season). An association study was performed on 250 Arabian horses to assess the effect of the SH3RF2:c.796 T > C (p.Ser266Pro) variant on race performance traits in flat gallop-racing.ResultsA gene expression analysis confirmed a significant decrease (p < 0.01) in the anti-apoptotic SH3RF2 (POSHER) gene during training periods that differed in intensity. The highest SH3RF2 expression level was detected in the muscles of untrained horses, whereas the lowest expression was identified at the end of the racing season in horses that were fully adapted to the exercise. A non-significant decrease in SH3RF1 gene expression following the training periods was observed. Moreover, a serine substitution by proline at amino acid position 266 (CC genotype) was negatively associated with the probability of winning races, the number of races in which a horse occurred and the financial value of the prizes. Horses with the TT genotype achieved the highest financial benefits, both for total winnings and for winnings per race in which the horses participated.ConclusionsThe present study showed the supposed regulation mechanism of exercise-induced apoptosis in horses at the molecular level. The identified SH3RF2: c.796 T > C missense variant was associated with selected racing performance traits, which is important information during the evaluation of horses’ exercise predisposition. The association results and frequencies of the CT and TT genotypes suggest the possibility of using SH3RF2 variant in selection to improve the racing performance of Arabian horses.Electronic supplementary materialThe online version of this article (10.1186/s12917-018-1567-0) contains supplementary material, which is available to authorized users.

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“…The biopsy of the gluteus medius muscle was performed using a minimally invasive ProMag Ultra Automatic Biopsy Instrument (Argon Medical Devices) with a 2 mm diameter biopsy needle according to the detailed procedure described by Stefaniuk et al (51). Muscle samples were obtained from the dorsal compartment of m. gluteus medius.…”

Section: Methodsmentioning

confidence: 99%

Exercise-induced modification of the skeletal muscle transcriptome in Arabian horses

Ropka‐Molik

Stefaniuk‐Szmukier

̇ukowski

et al. 2017

Physiological Genomics

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It has been found that Arabian and Thoroughbred horses differ in muscle fiber structure and thus in physiological changes occurring in muscles during exercise. The aim of the present study was to identify the global gene expression modifications that occur in skeletal muscle following a training regime to prepare for flat racing. Whole transcriptomes of muscle (gluteus medius) were compared between three time points of tissue collection: T (untrained horses), T (horses after intense gallop phase), and T (horses at the end of racing season), 23 samples in total. The numerous groups of exercise-regulated differentially expressed genes (DEGs) were related to muscle cell structure and signaling and included insulin-like growth factor 1 receptor (), insulin receptor (), transforming growth factor beta receptors 1 and 2 (), vascular endothelial growth factor B (); epidermal growth factor (), hepatocyte growth factor (), and vascular endothelial growth factor D (). In Arabian horses, exercise modified the expression of genes belonging to the PPAR signaling pathway (e.g., , and), calcium signaling pathway, and pathways associated with metabolic processes (e.g., oxidative phosphorylation, fatty acid metabolism, glycolysis/gluconeogenesis, and citrate cycle). According to detected gene expression modifications, our results suggested that in Arabian horses, exercise switches energy generation toward fatty acid utilization and enhances glycogen transport and calcium signaling. The use of the RNA-Seq approach in analyzing the skeletal muscle transcriptome allowed for the proposal of a panel of new candidate genes potentially related to body homeostasis maintenance and racing performance in Arabian horses.

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Evaluation of minimally invasive muscle biopsy method for genetic analysis in horse

Cited by 5 publications

References 16 publications

The Blood and Muscle Expression Pattern of the Equine TCAP Gene during the Race Track Training of Arabian Horses

The Blood and Muscle Expression Pattern of the Equine TCAP Gene during the Race Track Training of Arabian Horses

Molecular characterization of the apoptosis-related SH3RF1 and SH3RF2 genes and their association with exercise performance in Arabian horses

Exercise-induced modification of the skeletal muscle transcriptome in Arabian horses

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