Evaluation of Mitotic Figures and Cellular and Nuclear Morphometry of Various Histopathological Grades of Oral Squamous Cell Carcinoma: Comparative study using crystal violet and Feulgen stains

Kesarkar, Kashmira; Tamgadge, Avinash; Peirera, Treville; Tamgadge, Sandhya; Gotmare, Swati; Kamat, Pooja

doi:10.18295/squmj.2018.18.02.005

Cited by 9 publications

(5 citation statements)

References 18 publications

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“… 28 An increase in mitotic perimeter directly indicates CIN, as it reflects irregular mitotic division, often resulting in chromosomal rearrangements and potential genetic abnormalities. 29 Here, the mean perimeter of metaphases was higher in LS mucCRC as compared to LS mucADE and increased along with increasing malignancy of the samples ( Fig. 2 ).…”

Section: Discussionmentioning

confidence: 74%

“…Since chromosomal instability manifests as atypical mitoses, 28 we reasoned it could be demonstrated by measuring the size of mitoses as previously described by Kesarkar et al. 29 Feulgen staining with light green background stain was used as previously described 12 to visualize nuclear material and mitoses in 4 carcinoma (LS_CAR2, LS_CAR3, LS_CAR4, LS_CAR5), 3 adenoma (LS_ADE9, LS_ADE11, LS_ADE12), and 5 colonic mucosa samples from patients with LS diagnosed either with adenoma (LS_N9, LS_N23) or carcinoma (LS_N5, LS_N6, LS_N7) ( Supplementary Table S1 ). A sigmoid colon carcinoma previously confirmed as MMR proficient (pMMR) and CIN positive by comparative genomic hybridization study (case no.…”

Section: Methodsmentioning

confidence: 99%

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Mitotic abnormalities precede microsatellite instability in lynch syndrome-associated colorectal tumourigenesis

Pussila,

Laiho,

Törönen

et al. 2024

eBioMedicine

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Section: Discussionmentioning

confidence: 74%

Section: Methodsmentioning

confidence: 99%

Mitotic abnormalities precede microsatellite instability in lynch syndrome-associated colorectal tumourigenesis

Pussila,

Laiho,

Törönen

et al. 2024

eBioMedicine

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“…A proliferação excessiva de células devido ao aumento da mitoses atípicas é característica em lesões neoplásicas e pré-neoplásicas. Assim, avaliar quantitativamente o número de mitoses pode servir como um bom fator de prognóstico para tais lesões (Kesarkar et al 2018). A contagem de mitoses tem se revelado um importante fator na avaliação prognóstica para cães portadores de mastocitomas cutâneos (Romansik et al,2007).…”

Section: Resultsunclassified

Fatores prognósticos e seu papel na classificação histológica dos carcinoma de células escamosas cutâneos

Corrêa

Castro

Silva

et al. 2021

RSD

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O carcinoma de células escamosas (CCEs) é um tumor epitelial maligno de queratinócitos, composto por células heterogêneas com variados fenótipos, que ocorre principalmente em regiões glabras, com pouca ou nenhuma pigmentação. Ele é uma neoplasia comum em cães, gatos, equinos e bovinos, relativamente incomum em ovinos e rara em caprinos e suínos. Este estudo teve por objetivo realizar uma revisão crítica quanto aos diferentes sistemas de classificações histopatológicas de CCEs cutâneos e seu impacto na definição do prognóstico. A medicina humana conta com diversos sistemas de classificação para CCEs orais e cutâneos, como o sistema de Broders (1925) que propõe uma gradação baseada na diferenciação celular, e de Bryne (1992), que se baseia na graduação multifatorial de malignidade, avaliando quatro características morfológicas, para as quais são atribuídos escores e que após a soma dos escores, resultam em um grau. Em medicina veterinária, o sistema de classificação mais utilizado é ainda o de Weiss e Frese (1974), baseado no grau de diferenciação. Todavia, Nagamine et al. (2017) elaboraram um sistema de classificação multifatorial de graduação de malignidade para CCEs orais e cutâneos em cães, avaliando cinco características morfológicas para as quais são atribuídos escores (de 1 a 4) e somados, resultam num grau. Cada um dos sistemas se baseia e um ou vários fatores considerados como prognósticos. Esses sistemas de classificação podem apresentar vantagens e desvantagens, sendo necessário conhecer de forma aprofundada os diferentes aspectos de cada um para que se possa optar pelo que contemple a finalidade diagnóstica.

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“…The presence of mitotic activity and perineural invasion, as noted in our case, indicates a more aggressive behaviour and necessitates a comprehensive treatment approach. 10,11 Treatment strategies for MEC, particularly in minor salivary glands, revolve around surgical excision with clear margins, often supplemented by adjuvant radiotherapy for intermediate to high-grade tumors or those with adverse features. The therapeutic rationale parallels our management approach, advocating for a multidisciplinary strategy to optimize patient outcomes.…”

Section: Discussionmentioning

confidence: 99%

Mucoepidermoid Carcinoma of Minor Salivary gland Mimicking as Traumatic Fibroma: A Rare Case Report

et al. 2024

IJFMR

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This comprehensive case report details a rare presentation of Mucoepidermoid Carcinoma (MEC) in the minor salivary gland, manifesting with clinical characteristics remarkably similar to those of a benign fibroma. MEC, a predominant malignancy within the salivary gland neoplasms, primarily affects the major salivary glands, with occurrences in the minor glands being particularly rare and challenging to diagnose due to their indolent progression and atypical presentations. This report highlights the case of a 69-year-old female who presented with a painless, gradually enlarging mass in the floor of the mouth, initially presumed to be a traumatic fibroma based on its clinical appearance and location. Histopathological examination following an excisional biopsy revealed it to be intermediate-grade MEC, characterized by a mix of intermediate, epidermoid, and mucus-producing cells, along with notable mitotic activity and perineural invasion. Treatment includes surgical excision with clear margins and consideration of adjuvant radiotherapy, according to current guidelines for MEC management. This case underscores the critical importance of histopathological analysis in the differential diagnosis of oral lesions and the necessity for a high degree of clinical suspicion for malignancies in cases of atypical presentations. This case adds valuable insight into the diverse presentations of MEC and reinforces the importance of considering this diagnosis in the evaluation of persistent oral lesions, particularly when they mimic benign conditions.

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Evaluation of Mitotic Figures and Cellular and Nuclear Morphometry of Various Histopathological Grades of Oral Squamous Cell Carcinoma: Comparative study using crystal violet and Feulgen stains

Abstract: The Feulgen stain is more reliable than 1% crystal violet in terms of the selective staining of MFs. Moreover, the findings of this study indicate that computer-based morphometric analysis is an effective tool for differentiating between various grades of OSCC.

Cited by 9 publications

References 18 publications

Mitotic abnormalities precede microsatellite instability in lynch syndrome-associated colorectal tumourigenesis

Mitotic abnormalities precede microsatellite instability in lynch syndrome-associated colorectal tumourigenesis

Fatores prognósticos e seu papel na classificação histológica dos carcinoma de células escamosas cutâneos

Mucoepidermoid Carcinoma of Minor Salivary gland Mimicking as Traumatic Fibroma: A Rare Case Report

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