Evaluation of MTBH, a novel hesperetin derivative, on the activity of hepatic cytochrome P450 isoform <i>in vitro</i> and <i>in vivo</i> using a cocktail method by HPLC-MS/MS

Miss, Yan Qin; Dong, Haijun; Miss, Jiayin Sun; Zhang, Yilong; Prof., Jun Li; Mr., Tianci Zhang; Chen, Guanjun; Wang, Sheng; Song, Shuai; Wang, Wei; Fan, Yuru; Wang, Jie; Huang, Xiaohui; Shen, Chao

doi:10.1080/00498254.2021.2009934

Cited by 1 publication

(2 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mice in the HFD + OP-D group continued to be fed with HFD and were intragastrically administered with 5 mg/kg/day OP-D (dissolved in 0.5% of carboxymethyl cellulose) for the following 4 weeks, according to preliminary experiments and previous studies ( 14 , 15 ). Mice in the other groups were treated with the same volume of 0.5% sodium carboxyl methyl cellulose solution (in order to protect the gastric mucosa of mice) according to a previous study ( 16 , 17 ). At the end of the experiment (16 weeks), all mice were sacrificed by cervical dislocation.…”

Section: Methodsmentioning

confidence: 99%

“…(14,15). Mice in the other groups were treated with the same volume of 0.5% sodium carboxyl methyl cellulose solution (in order to protect the gastric mucosa of mice) according to a previous study (16,17). At the end of the experiment (16 weeks), all mice were sacrificed by cervical dislocation.…”

Section: Ophiopogonin D Ameliorates Non-alcoholic Fatty Liver Disease...mentioning

confidence: 99%

See 1 more Smart Citation

Ophiopogonin D ameliorates non‑alcoholic fatty liver disease in high‑fat diet‑induced obese mice by improving lipid metabolism, oxidative stress and inflammatory response

Huang

She

et al. 2023

Exp Ther Med

View full text Add to dashboard Cite

Lipid metabolic disorders, oxidative stress and inflammation in the liver are key steps in the progression of non-alcoholic fatty liver disease (NAFLD). Ophiopogonin D (OP-D), the main active ingredient of Ophiopogon japonicus , exhibits several pharmacological activities such as antioxidant and anti-inflammatory activities. Therefore, the current study aimed to explore the role of OP-D in NAFLD in a high-fat diet (HFD)-induced obesity mouse model. To investigate the effect of OP-D on NAFLD in vivo , a NAFLD mouse model was established following feeding mice with HFD, then the mice were randomly treated with HFD or HFD + OP-D for 4 weeks. Subsequently, primary mouse hepatocytes were isolated, and enzyme-linked immunosorbent assay, reverse transcription-quantitative PCR western blotting and immunofluorescence analysis were used for assessment to explore the direct effect of OP-D in vitro . The results of the present study indicated that OP-D could ameliorate NAFLD in HFD-induced obese mice by regulating lipid metabolism and antioxidant and anti-inflammatory responses. Additionally, OP-D treatment decreased lipogenesis and inflammation levels in vitro , suggesting that the NF-κB signaling pathway may be involved in the beneficial effects of OP-D on NAFLD.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Ophiopogonin D Ameliorates Non-alcoholic Fatty Liver Disease...mentioning

confidence: 99%

Ophiopogonin D ameliorates non‑alcoholic fatty liver disease in high‑fat diet‑induced obese mice by improving lipid metabolism, oxidative stress and inflammatory response

Huang

She

et al. 2023

Exp Ther Med

View full text Add to dashboard Cite

show abstract

Evaluation of MTBH, a novel hesperetin derivative, on the activity of hepatic cytochrome P450 isoform in vitro and in vivo using a cocktail method by HPLC-MS/MS

Cited by 1 publication

References 35 publications

Ophiopogonin D ameliorates non‑alcoholic fatty liver disease in high‑fat diet‑induced obese mice by improving lipid metabolism, oxidative stress and inflammatory response

Ophiopogonin D ameliorates non‑alcoholic fatty liver disease in high‑fat diet‑induced obese mice by improving lipid metabolism, oxidative stress and inflammatory response

Contact Info

Product

Resources

About