Evaluation of multiphase implants for repair of focal osteochondral defects in goats

Niederauer, G. G.; Slivka, Michael A.; Leatherbury, N.C.; Korvick, Donna L.; Harroff, H. H.; Ehler, W.; Dunn, C. J.; Kieswetter, Kris

doi:10.1016/s0142-9612(00)00124-1

Cited by 247 publications

(168 citation statements)

References 52 publications

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“…To investigate the stability of the integrated layers, interfacial strength at the interfaces between each layer was assessed. Poor interfacial strength leads to delamination of the layers, a problem widely reported in the fabrication of layered scaffold materials for tissue engineering [15,26,27]. Using a custom layer adhesion strength measurement technique, the interfacial layer strength was tested to failure, with delamination of the layers being found to occur within the confines of the mechanically weakest layer (top cartilage layer) rather than at the interface.…”

Section: Discussionmentioning

confidence: 99%

“…The first generation of constructs involved fabrication of two separate scaffolds; one designed to repair the cartilage tissue and the other designed to repair bone tissue. These two scaffolds were subsequently fused together using sutures [25,25] or biological sealants or glues [19,26,27]. Success of such materials has been limited due to poor cellular infiltration through the layers of the structure.…”

Section: Introductionmentioning

confidence: 99%

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A biomimetic multi-layered collagen-based scaffold for osteochondral repair

et al. 2014

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A biomimetic multi-layered collagen-based scaffold for osteochondral repair

et al. 2014

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“…PLGA was chosen because (A) its proven biocompatibility, (B) the feasibility of threedimensional molding of the constructs in the shape of the experimental defects, (C) it allowed minimally invasive arthroscopic implantation and (D) its degradation rate, which was compatible with the expected deposition of ECM by the cells loaded on the scaffolds (Villalobos Córdoba et al 2007;Niederauer et al 2000;Sittinger et al 1996;Uematsu et al 2005). Moreover, the use of porous PLGA scaffolds permitted the retention of the cells at the defect site and promoted homogeneous distribution throughout the graft.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, PLGA-based constructs allow arthroscopic implantation, making this biomaterial even more attractive for clinical use, since the arthroscopic technique reduces morbidity, surgical time, patient recovery and secondary complications typically derived from open surgery that are routinely performed in tissue engineering strategies for cartilage repair (Villalobos Córdoba et al 2007;Erggelet et al 2007;Niederauer et al 2000). Although in some samples the tide mark was faint or disappeared, it is remarkable that none of the specimens showed detachment of neocartilage from subchondral bone, indicating that the integration between the two layers resisted the load bearing forces in all treatment groups and highlighting the suitability of PLGA scaffolds in the treatment of osteochondral defects.…”

Section: Discussionmentioning

confidence: 99%

Cartilage resurfacing potential of PLGA scaffolds loaded with autologous cells from cartilage, fat, and bone marrow in an ovine model of osteochondral focal defect

et al. 2015

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Current developments in tissue engineering strategies for articular cartilage regeneration focus on the design of supportive three-dimensional scaffolds and their use in combination with cells from different sources. The challenge of translating initial successes in small laboratory animals into the clinics involves pilot studies in large animal models, where safety and efficacy should be investigated during prolonged follow-up periods. Here we present, in a single study, the long-term (up to 1 year) effect of biocompatible porous scaffolds non-seeded and seeded with fresh ex vivo expanded autologous progenitor cells that were derived from three different cell sources [cartilage, fat and bone marrow (BM)] in order to evaluate their advantages as cartilage resurfacing agents. An ovine model of critical size osteochondral focal defect was used and the test items were implanted arthroscopically into the knees. Evidence of regeneration of hyaline quality tissue was observed at 6 and 12 months post-treatment with variable success depending on the cell source. Cartilage and BM-derived mesenchymal stromal cells (MSC), but not those derived from fat, resulted in the best quality of new cartilage, as judged qualitatively by magnetic resonance imaging and macroscopic assessment, and by histological quantitative scores. Given the limitations in sourcing cartilage tissue and the risk of donor site morbidity, BM emerges as a preferential source of MSC for novel cartilage resurfacing therapies of osteochondral defects using copolymeric poly-D,L-lactide-co-glycolide scaffolds.

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“…Published studies used arthrotomy procedures to implant the tissue engineered constructs (Córdoba et al 2007;Erggelet et al 2007;Niederauer et al 2000), because the difficulty of holding in place the implant without invasive procedures. The biomechanical characteristics of some of the constructs, as the ones made of chondrocytes and fibrin are too soft to hold in the defect site independently of the use of a periosteum patch.…”

Section: Discussionmentioning

confidence: 99%

An arthroscopic approach for the treatment of osteochondral focal defects with cell-free and cell-loaded PLGA scaffolds in sheep

Fonseca¹,

Caminal

Peris

et al. 2013

Cytotechnology

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Osteochondral injuries are common in humans and are relatively difficult to manage with current treatment options. The combination of novel biomaterials and expanded progenitor or stem cells provides a source of therapeutic and immunologically compatible medicines that can be used in regenerative medicine. However, such new medicinal products need to be tested in translational animal models using the intended route of administration in humans and the intended delivery device. In this study, we evaluated the feasibility of an arthroscopic approach for the implantation of biocompatible copolymeric poly-D,Llactide-co-glycolide (PLGA) scaffolds in an ovine preclinical model of knee osteochondral defects.Moreover this procedure was further tested using ex vivo expanded autologous chondrocytes derived from cartilaginous tissue, which were loaded in PLGA scaffolds and their potential to generate hyaline cartilage was evaluated. All scaffolds were successfully implanted arthroscopically and the clinical evolution of the animals was followed by non invasive MRI techniques, similar to the standard in human clinical practice. No clinical complications occurred after the transplantation procedures in any of the animals. Interestingly, the macroscopic evaluation demonstrated significant improvement after treatment with scaffolds loaded with cells compared to untreated controls.C. Fonseca, M. Caminal and D. Peris contributed equally to this work.Electronic supplementary material The online version of this article

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Evaluation of multiphase implants for repair of focal osteochondral defects in goats

Cited by 247 publications

References 52 publications

A biomimetic multi-layered collagen-based scaffold for osteochondral repair

A biomimetic multi-layered collagen-based scaffold for osteochondral repair

Cartilage resurfacing potential of PLGA scaffolds loaded with autologous cells from cartilage, fat, and bone marrow in an ovine model of osteochondral focal defect

An arthroscopic approach for the treatment of osteochondral focal defects with cell-free and cell-loaded PLGA scaffolds in sheep

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