Evaluation of nanoplastics toxicity in the soil nematode Caenorhabditis elegans by iTRAQ-based quantitative proteomics

Huang, Gefei; Ma, Yiming; Xie, Dongying; Zhao, Cunmin; Zhu, Lin; Xie, Guangshan; Wu, Pengfei; Wang, Wei; Zhao, Zhongying; Cai, Zongwei

doi:10.1016/j.scitotenv.2022.160646

Cited by 10 publications

(5 citation statements)

References 70 publications

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“…Many bacterivores, for instance, can ingest particles larger than 0.5 µm but with an upper limit of 6 µm ( Fueser et al, 2019 ), whereas nematodes with other feeding strategies may also ingest considerably larger-sized particles ( Moens et al, 2014 ). These particles accumulate primarily in the pharynx and intestine of nematodes ( Fueser et al, 2022 ; Fueser et al, 2019 ; Huang et al, 2023 ; this study). Numbers of particles in the worms’ guts tend to rise with exposure time ( Quanbin et al, 2020 ; Shang et al, 2020 ; Zhang et al, 2019 ; this study), albeit to a limited extent.…”

Section: Discussionmentioning

confidence: 69%

Short-term microplastic effects on marine meiofauna abundance, diversity and community composition

de França,

Moens,

da Silva

et al. 2024

PeerJ

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Background Due to the copious disposal of plastics, marine ecosystems receive a large part of this waste. Microplastics (MPs) are solid particles smaller than 5 millimeters in size. Among the plastic polymers, polystyrene (PS) is one of the most commonly used and discarded. Due to its density being greater than that of water, it accumulates in marine sediments, potentially affecting benthic communities. This study investigated the ingestion of MP and their effect on the meiofauna community of a sandy beach. Meiofauna are an important trophic link between the basal and higher trophic levels of sedimentary food webs and may therefore be substantially involved in trophic transfer of MP and their associated compounds. Methods We incubated microcosms without addition of MP (controls) and treatments contaminated with PS MP (1-µm) in marine sediments at three nominal concentrations (103, 105, 107particles/mL), for nine days, and sampled for meiofauna with collections every three days. At each sampling time, meiofauna were collected, quantified and identified to higher-taxon level, and ingestion of MP was quantified under an epifluorescence microscope. Results Except for Tardigrada, all meiofauna taxa (Nematoda, turbellarians, Copepoda, Nauplii, Acari and Gastrotricha) ingested MP. Absorption was strongly dose dependent, being highest at 107 particles/mL, very low at 105 particles/mL and non-demonstrable at 103 particles/mL. Nematodes accumulated MP mainly in the intestine; MP abundance in the intestine increased with increasing incubation time. The total meiofauna density and species richness were significantly lower at the lowest MP concentration, while at the highest concentration these parameters were very similar to the control. In contrast, Shannon-Wiener diversity and evenness were greater in treatments with low MP concentration. However, these results should be interpreted with caution because of the low meiofauna abundances at the lower two MP concentrations. Conclusion At the highest MP concentration, abundance, taxonomic diversity and community structure of a beach meiofauna community were not significantly affected, suggesting that MP effects on meiofauna are at most subtle. However, lower MP concentrations did cause substantial declines in abundance and diversity, in line with previous studies at the population and community level. While we can only speculate on the underlying mechanism(s) of this counterintuitive response, results suggest that further research is needed to better understand MP effects on marine benthic communities.

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Section: Discussionmentioning

confidence: 69%

Short-term microplastic effects on marine meiofauna abundance, diversity and community composition

de França,

Moens,

da Silva

et al. 2024

PeerJ

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“…Such C. elegans proteins have been related to translation, ribosome biogenesis, proteolysis, kinases, protein processing in the endoplasmic reticulum, and energy metabolism. Remarkably, the changes in proteome followed by nanopolystyrene exposure are corroborated by the phenotypic impacts observed in C. elegans [ 187 ].…”

Section: Caenorhabditis Elegans and Nanoplasticsmentioning

confidence: 97%

Caenorhabditis elegans as a Prediction Platform for Nanotechnology-Based Strategies: Insights on Analytical Challenges

Gubert

Oliveira

et al. 2023

Toxics

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Nanotechnology-based strategies have played a pivotal role in innovative products in different technological fields, including medicine, agriculture, and engineering. The redesign of the nanometric scale has improved drug targeting and delivery, diagnosis, water treatment, and analytical methods. Although efficiency brings benefits, toxicity in organisms and the environment is a concern, particularly in light of global climate change and plastic disposal in the environment. Therefore, to measure such effects, alternative models enable the assessment of impacts on both functional properties and toxicity. Caenorhabditis elegans is a nematode model that poses valuable advantages such as transparency, sensibility in responding to exogenous compounds, fast response to perturbations besides the possibility to replicate human disease through transgenics. Herein, we discuss the applications of C. elegans to nanomaterial safety and efficacy evaluations from one health perspective. We also highlight the directions for developing appropriate techniques to safely adopt magnetic and organic nanoparticles, and carbon nanosystems. A description was given of the specifics of targeting and treatment, especially for health purposes. Finally, we discuss C. elegans potential for studying the impacts caused by nanopesticides and nanoplastics as emerging contaminants, pointing out gaps in environmental studies related to toxicity, analytical methods, and future directions.

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“…Thus, in the context of autophagy modulation, the focus was put on genes such as vha-5, cpr-5, and epg-8; vha-5 is a gene that encodes vacuolar (H + )-ATPase, which maintains lysosomal acidity and degradation competence [53], while cpr-5 is an ortholog of human cathepsin B (CatB), a cysteine-type endopeptidase that is located in lysosomes and promotes cellular proteolysis [54]. Furthermore, the expression of epg-8, a gene related to the PI3K complex, was also checked, as it has been described that autophagosome reduction by the phosphoinositide 3-kinase (PI3K) complex delayed Aβ-induced paralysis in the CL4176 strain and alleviated Aβ-induced toxicity, causing a neuroprotective effect [30].…”

Section: Selection Of Genesmentioning

confidence: 99%

Insights into the Neuroprotective Potential of Epicatechin: Effects against Aβ-Induced Toxicity in Caenorhabditis elegans

Ayuda-Durán,

Garzón-García,

González-Manzano

et al. 2024

Antioxidants

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Medical therapies to avoid the progression of Alzheimer’s disease (AD) are limited to date. Certain diets have been associated with a lower incidence of neurodegenerative diseases. In particular, the regular intake of foods rich in polyphenols, such as epicatechin (EC), could help prevent or mitigate AD progression. This work aims to explore the neuroprotective effects of EC using different transgenic strains of Caenorhabditis elegans, which express human Aβ1-42 peptides and contribute to elucidating the mechanisms involved in the effects of EC in AD. The performed assays indicate that this flavan-3-ol was able to reduce the signs of β-amyloid accumulation in C. elegans, improving motility and chemotaxis and increasing survival in transgenic strain peptide producers compared to nematodes not treated with EC. The neuroprotective effects exhibited by EC in C. elegans could be explained by the modulation of inflammation and stress-associated genes, as well as autophagy, microgliosis, and heat shock signaling pathways, involving the regulation of cpr-5, epg-8, ced-7, ZC239.12, and hsp-16 genes. Overall, the results obtained in this study support the protective effects of epicatechin against Aβ-induced toxicity.

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Evaluation of nanoplastics toxicity in the soil nematode Caenorhabditis elegans by iTRAQ-based quantitative proteomics

Cited by 10 publications

References 70 publications

Short-term microplastic effects on marine meiofauna abundance, diversity and community composition

Short-term microplastic effects on marine meiofauna abundance, diversity and community composition

Caenorhabditis elegans as a Prediction Platform for Nanotechnology-Based Strategies: Insights on Analytical Challenges

Insights into the Neuroprotective Potential of Epicatechin: Effects against Aβ-Induced Toxicity in Caenorhabditis elegans

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