Evaluation of new commercial immunochromatography kit for norovirus in feces

Mutoh, Kozo; Hakamata, Akihiro; Yagi, Hideya; Kurokawa, Keiji; Miki, Naoki; Kurita, Izumi

doi:10.1111/j.1442-200x.2008.02788.x

Cited by 10 publications

(7 citation statements)

References 10 publications

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“…IC kits have also been developed recently in Japan for the detection of NoV in fecal specimens by three separate companies, Immuno‐Probe, Morinaga Milk Industry, and Denka Seiken. These kits have been evaluated previously by examining fecal specimens from Japan [Khamrin et al, 2008, 2009; Takanashi et al, 2008; Mutoh et al, 2009] and Vietnam [Nguyen et al, 2007].…”

Section: Sensitivity Specificity and Agreement Of The Ip‐nov Kit Anmentioning

confidence: 99%

Evaluation of an immunochromatography method for rapid detection of noroviruses in clinical specimens in Thailand

Thongprachum

Khamrin

Chaimongkol

et al. 2010

Journal of Medical Virology

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Norovirus (NoV) is a causative agent of gastroenteritis in children and adults worldwide. Although reverse transcription-polymerase chain reaction (RT-PCR) has been accepted as the standard method for diagnosis of NoV infection, it requires well-trained personnel and sophisticated equipments. Performance of a commercial immunochromatography (IC) test for rapid detection of NoV was evaluated with fecal specimens collected from children admitted to a hospital with acute gastroenteritis during 2005-2007 in Chiang Mai, Thailand. A total of 463 fecal specimens were tested for the presence of NoV by a commercial immunochromatography kit (IP-NoV) and by RT-PCR. Sensitivity, specificity, and agreement of immunochromatography as compared to RT-PCR were 74.2%, 99.5%, and 96.1%, respectively. Based on the NoV genotypes determined by phylogenetic analysis, immunochromatography detected NoV GII/3, GII/4, GII/6, GII/13, GII/15, and GII/16 genotypes. The findings indicate that the immunochromatography kit could be used for a direct detection of NoV GII in clinical specimens and covering a wide range of NoV genotypes circulating in Thailand.

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Section: Sensitivity Specificity and Agreement Of The Ip‐nov Kit Anmentioning

confidence: 99%

Evaluation of an immunochromatography method for rapid detection of noroviruses in clinical specimens in Thailand

Thongprachum

Khamrin

Chaimongkol

et al. 2010

Journal of Medical Virology

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“…A simple approach with minimal hands-on time is more applicable and requisite as a screening assay. There were several commercial immunoassays for HuNoV detection available, such as Quick Ex-Norovirus, RIDA® QUICK (N1402), Immuno Search NV kit, NOROTOP+, and SD Bioline Norovirus [34][35][36][37][38][39][40]. However, these assays performed better for a single genotype of HuNoVs.…”

Section: Discussionmentioning

confidence: 99%

Broad-range and Effective Detection of Human Noroviruses by Colloidal Gold Immunochromatographic Assay Based on the Shell Domain of the Major Capsid Protein

et al. 2020

Preprint

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Background: Human noroviruses (HuNoVs) are a major cause of nonbacterial gastroenteritis in all ages worldwide. As the replication of HuNoVs in vitro is immature, the detection of HuNoVs is depended on molecular assays such as RT-PCR and RT-qPCR. However, these molecular-based techniques require special equipment, unique reagents, experienced operators to perform, and extended time to get results. In addition, the diversity of viral genotypes is high. Therefore, a method for rapidly, broad-range, and effective detectionf of HuNoVs was desiderated for screening the excrement or vomit from infected people when outbreaks occur.Results: In this study, a colloidal-gold-based immunochromatographic assay (ICA) was developed for highly effective detection of HuNoVs in clinical samples. Monoclonal antibodies (MAbs) against the shell (S) domain in the major capsid protein of HuNoVs were used in the ICA. The limitations of detection for HuNoVs in clinical samples were 1.2×106 genomic copies per gram of stool sample (gc/g) and 4.4×105 gc/g for genogroup I and II (GI and GII) HuNoVs, respectively. A total of 122 clinical samples were tested for HuNoVs by ICA and compared against that by RT-qPCR. The relative sensitivity, specificity and agreement of the ICA was 84.2 % (95% CI: 83.6-84.8 %), 100.0 % (95 % CI: 98.5-100.0 %) and 87.7 % (95% CI: 85.6-89.8 %), respectively. No cross-reaction with other common enteric viruses or bacteria was observed. The ICA could detect a broad range of genotypes, including GI.1, GI.3, GI.4, GI.6, GI.14, GII.2, GII.3, GII.4, GII.6, GII.13, and GII.17 HuNoVs.Conclusions: Our results demonstrated that ICA targeting the S domain of VP1 is a promising candidate for effectively improve identifying different genotypes of HuNoVs in clinical samples with high sensitivity and specificity.

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“…A simple approach with minimal hands-on time is more applicable and requisite as a screening assay. There were several commercial HuNoV detection kits available, such as Quick Ex-Norovirus, RIDA® QUICK (N1402), Immuno Search NV kit, NOROTOP+, and SD Bioline Norovirus [28][29][30][31][32][33][34]. However, these assays performed better for a single genotype.…”

Section: Discussionmentioning

confidence: 99%

Broad-range and Effective Detection of Human Noroviruses by Colloidal Gold Immunochromatographic Assay Based on the Shell Domain of the Major Capsid Protei

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Human noroviruses (HuNoVs) are a major cause of nonbacterial gastroenteritis in all ages worldwide. As the replication of HuNoVs in vitro is immature, the detection of HuNoVs is depended on molecular assays such as RT-PCR and RT-qPCR. However, these molecular-based techniques require special equipment, unique reagents, experienced operators to perform, and extended time to get results. In addition, the genotypes of HuNoVs are broad. Therefore, a method for rapidly, broad-range, and effective approach for HuNoVs would be more applicable for screening infected people when outbreaks occur.Results: In this study, a colloidal-gold-based immunochromatographic assay (ICA) was developed for highly effective detection of HuNoVs in clinical samples. Monoclonal antibodies (McAbs) against the shell (S) domain in the major capsid protein of HuNoV were used in the ICA. The limitations of detection for HuNoVs in clinical samples were 1.2×106 genomic copies per gram (gc/g) and 4.4×105 gc/g for genogroup I and II (GI and GII) HuNoVs, respectively. A total of 122 samples were tested for HuNoVs by ICA and compared against that by RT-qPCR. The relative sensitivity, specificity and agreement of the ICA was 84.2 % (95% CI: 83.6-84.8 %), 100.0 % (95 % CI: 98.5-100.0 %) and 87.7 % (95% CI: 85.6-89.8 %), respectively. No cross-reaction with other common enteric viruses or bacteria was observed. The ICA could detect a broad range of genotypes, including GI.1, GI.3, GI.4, GI.6, GI.14, GII.2, GII.3, GII.4, GII.6, GII.13, and GII.17 HuNoVs.Conclusions: Our results demonstrated that ICA targeting the S domain protein is a promising candidate for effectively improve identifying different genotypes of HuNoVs in clinical samples with high sensitivity and specificity.

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Evaluation of new commercial immunochromatography kit for norovirus in feces

Cited by 10 publications

References 10 publications

Evaluation of an immunochromatography method for rapid detection of noroviruses in clinical specimens in Thailand

Evaluation of an immunochromatography method for rapid detection of noroviruses in clinical specimens in Thailand

Broad-range and Effective Detection of Human Noroviruses by Colloidal Gold Immunochromatographic Assay Based on the Shell Domain of the Major Capsid Protein

Broad-range and Effective Detection of Human Noroviruses by Colloidal Gold Immunochromatographic Assay Based on the Shell Domain of the Major Capsid Protei

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