Evaluation of nose-only aerosol inhalation chamber and comparison of experimental results with mathematical simulation of aerosol deposition in mouse lungs

Nadithe, Venkatareddy; Rahamatalla, Muhib; Finlay, Warren H.; Mercer, John R.; Samuel, John

doi:10.1002/jps.10379

Cited by 52 publications

(51 citation statements)

References 40 publications

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“…The infections were performed 1 day after the final CPA injection (after a reduction of approximately 5% from the average starting mouse weight). The mice were infected with either B. cenocepacia (K56-2 or C6433) or mock in- fected with gHBSS using the NOID, as described previously (44). The mice were anesthetized with a ketamine (0.85 mg/g of body weight; Bimeda MTC) and xylazine (0.1 mg/g of body weight; Rompun; Bayer HealthCare) mixture administered by i.p.…”

Section: Methodsmentioning

confidence: 99%

Aerosol Phage Therapy Efficacy in Burkholderia cepacia Complex Respiratory Infections

Semler

Goudie

Finlay

et al. 2014

Antimicrob Agents Chemother

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Phage therapy has been suggested as a potential treatment for highly antibiotic-resistant bacteria, such as the species of the Burkholderia cepacia complex (BCC). To address this hypothesis, experimental B. cenocepacia respiratory infections were established in mice using a nebulizer and a nose-only inhalation device. Following infection, the mice were treated with one of five B. cenocepacia-specific phages delivered as either an aerosol or intraperitoneal injection. The bacterial and phage titers within the lungs were assayed 2 days after treatment, and mice that received the aerosolized phage therapy demonstrated significant decreases in bacterial loads. Differences in phage activity were observed in vivo. Mice that received phage treatment by intraperitoneal injection did not demonstrate significantly reduced bacterial loads, although phage particles were isolated from their lung tissue. Based on these data, aerosol phage therapy appears to be an effective method for treating highly antibiotic-resistant bacterial respiratory infections, including those caused by BCC bacteria.

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Section: Methodsmentioning

confidence: 99%

Aerosol Phage Therapy Efficacy in Burkholderia cepacia Complex Respiratory Infections

Semler

Goudie

Finlay

et al. 2014

Antimicrob Agents Chemother

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“…Most animal inhalation studies quantify the exposure concentration; however, there are relatively few that quantitatively measure respiratory tract deposition (Raabe et al 1988;Yeh et al 1990;Nadithe et al 2003;Hofstetter et al 2004;Oldham et al 2004;Wichers et al 2006;Alessandrini et al 2008). A few of these studies have attempted to verify dosimetry model predictions.…”

Section: Introductionmentioning

confidence: 99%

“…A few of these studies have attempted to verify dosimetry model predictions. Nadithe et al (2003) designed a nose-only exposure system and used a proprietary dosimetry code to compare experimental and predicted particle deposition for 3.9 (±0.5) micrometer diameter particles. Wichers et al (2006) compared experimentally measured deposition with predictions from the MPPD dosimetry code (MPPD 2002) for 1.95 micrometer diameter particles.…”

Section: Introductionmentioning

confidence: 99%

“…Neither of these studies were able to utilize strain specific respiratory tract anatomy and respiratory physiology parameters. In a study by Nadithe et al (2003) C57BL/6 mice were exposed but the dosimetry modeling used a combination of CD-1 and B6C3F 1 mouse respiratory data. Their dosimetry predictions did not differ significantly from their in-vivo measurements.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Predicted and Experimentally Measured Aerosol Deposition Efficiency in BALB/C Mice in a New Nose-Only Exposure System

Oldham

Phalen

Budiman³

2009

Aerosol Science and Technology

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The use of respiratory tract dosimetry predictions to estimate the desired aerosol exposure concentration required for a specific target deposited dose in animal inhalation studies has been hindered by: (1) a lack of species/strain specific quantitative respiratory tract anatomy; and (2) verification by comparison of calculated and experimentally measured deposited doses. Using recent literature on tracheobronchial and pulmonary respiratory tract anatomy, dosimetry predictions for the Balb/c mouse were compared with deposited doses for 0.5, 1.0, and 2.0 micrometer diameter aerosols in a newly available nose-only exposure system. Spatial and temporal aerosol port to port uniformity of this noseonly exposure system was within ± 10% of the mean. Dosimetry predictions were in agreement with the measured mean deposited doses for the aerosols tested.

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“…Aerosol inhalation is a direct method of drug administration for treatment of respiratory diseases (1,2). The great advantage lies in the fact that the drug deposited from inhalation results in a high concentration at the site of action.…”

Section: Introductionmentioning

confidence: 99%

High-Frequency Ultrasonic Atomization for Drug Delivery to Rodent Animal Models—optimal Particle Size for Lung Inhalation of Difluoromethyl Ornithine

Zhang

Fandrey

Naqwi

et al. 2008

Experimental Lung Research

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A high (8 MHz) and low (1.7 MHz) frequency ultrasonic transducer were compared for delivering aerosols to mouse lung. The aerosol concentration (mass of dry particles/volume of air) rose nonlinearly with solution concentration of difluoromethyl ornithine for both transducers. The particle size was linear with the cube root of the solution concentration, and the slope of the low frequency transducer was eight times greater than that of the high frequency transducer. The deposition fraction assessed by the assayed mass in the lung relative to the calculated inhaled mass was found to decline exponentially with particle size. The lower frequency transducer provided a higher dose despite a lower deposition fraction, but the high frequency transducer was more efficient and provides a more selective deposition in the lower respiratory tract while operating with significantly less demands on aerosol drying.

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Evaluation of nose-only aerosol inhalation chamber and comparison of experimental results with mathematical simulation of aerosol deposition in mouse lungs

Cited by 52 publications

References 40 publications

Aerosol Phage Therapy Efficacy in Burkholderia cepacia Complex Respiratory Infections

Aerosol Phage Therapy Efficacy in Burkholderia cepacia Complex Respiratory Infections

Comparison of Predicted and Experimentally Measured Aerosol Deposition Efficiency in BALB/C Mice in a New Nose-Only Exposure System

High-Frequency Ultrasonic Atomization for Drug Delivery to Rodent Animal Models—optimal Particle Size for Lung Inhalation of Difluoromethyl Ornithine

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