Evaluation of novel alginate dialdehyde cross-linked chitosan/calcium polyphosphate composite scaffolds for meniscus tissue engineering

pH‐ and Redox‐Responsive Polysaccharide‐Based Microcapsules with Autofluorescence for Biomedical Applications

“…Alginate dialdehyde (ADA) was prepared by oxidization of alginate and subsequent precipitation with ethanol, according to the previously reported method. [30] Preparation of MnCO 3 and porous CaCO 3 microspheres: MnCO 3 microparticles with average diameter of 3 mm were synthesized by mixing MnSO 4 and NH 4 HCO 3 solutions according to a previously reported method. [41] CaCO 3 particles with average diameter of 2 mm were prepared by a typical method.…”

“…Moreover, the intensity of the peak at 1407 cm À1 after cross-linking is increased substantially, which lead to the merging of the peaks at 1424, 1405 and 1381 cm À1 , owing to the formation of the Schiff base (characteristic of the C=N).The peak at 2922 cm À1 in curve D (Figure 2) shows the CÀH stretching in the Schiff base. [28][29][30] It is notable that the presence of a band at 1734 cm À1 indicates the existence of free aldehyde groups in (ADA/CM) 5 microcapsules. All these changes demonstrate that the aldehyde groups in ADA are combined with amine groups in CM by a covalent Schiff base bond.…”

Carbon nanofillers incorporated electrically conducting poly ε-caprolactone nanocomposite films and their biocompatibility studies using MG-63 cell line

“…The cell viability on nanocomposite scaffolds was estimated using the standard MTT assay [41]. This method helps in evaluating the ability of mitochondrial dehydrogenase enzymes of viable cells present in the scaffolds/plate to reduce the yellow-colored tetrazolium salt into in soluble purple-colored formazan crystals.…”

In vitro cytocompatibility evaluation of alginate dialdehyde for biological tissue fixation

“…Alginate (ALG), an important naturally occurring carbohydrate polymer derived from brown algae, has been widely used in a variety of biomedical applications, mainly due to its high biocompatibility and nontoxicity (Goh, Heng, & Chan, 2011;Isiklan, Inal, Kursun, & Ercan, 2010;Knill et al, 2004;Li, Ramay, Hauch, Xiao, & Zhang, 2005;Wang et al, 2010;Yang, Xie, & He, 2011). In recent years, it was reported that alginate can be oxidized with periodate to produce entity with multiple functional aldehyde groups (aldehyde alginate, ADA) (Bouhadir et al, 2001).…”