Evaluation of nutritive capacities of 10 broth media

Abstract

Ten broth media were evaluated for their ability to support the growth of low inocula of selected fastidious aerobic, microaerophilic, and anaerobic bacteria. Thioglycolate medium USP (Oxoid, Basingstoke, England), cooked meat broth (Oxoid), and fastidious anaerobe broth (Quelab Laboratories Inc., Montreal, Quebec, Canada) were best able to support the growth of the greatest variety of organisms at the lowest inoculum tested (10 1 CFU). In most cases, incubation of these broths resulted in visible turbidity within 3 days. These three broth media were found to be equally suitable for use as an enrichment broth in the clinical laboratory.The use of enrichment broth media is recommended in order to enhance the recovery of pathogenic microorganisms, especially those with more fastidious growth requirements, from clinical specimens (1,4,6). Many broth media are available for the isolation of bacteria, but few studies comparing their usefulness have been published (3,5,7,8). In the study described here, a variety of broth media were evaluated for their ability to support the growth of low inocula of common and fastidious microorganisms.The broth media that were prepared in house and dispensed in 15-ml aliquots included (i) brain heart infusion (BHI; Becton Dickinson Microbiology Systems, Cockeysville, Md.); (ii) BHI with BIO-X (BHI-Bio), a supplement for fastidious organisms (Quelab Laboratories, Inc., Montreal, Quebec, Canada); (iii) thioglycolate medium USP (THIO; Oxoid, Basingstoke, England); (iv) Wilkins-Chalgren anaerobe broth (WC; Oxoid); and (v) cooked meat broth, consisting of WC base and cooked meat pellets (CM; Oxoid). Broth media that were obtained commercially in either 8-or 10-ml aliquots included (i) BHI anaerobe broth with 5% sheep blood (BHI-B; Quelab Laboratories, Inc.); (ii) fastidious anaerobe broth (FAB; Quelab Laboratories, Inc.); (iii) cooked meat enriched broth (CM-EB; Quelab Laboratories, Inc.); (iv) Schaedler broth (SB; Carr-Scarborough Microbiologicals, Inc., Stone Mountain,

Use of blood culture medium enrichment for synovial fluid culture in horses: A comparison of different culture methods

“…However, since low volume anaerobic BACTEC culture bottles are yet not available, other culture methods were optimised to promote their growth. Briefly, every agar culture was performed in duplicate using a rich agar (SCH) incubated in an anaerobe pouch system while thioglycolate was chosen as conventional enrichment broth because of its excellent recovery of anaerobes from human sterile body fluids (Reinhold et al 1988;Scythes et al 1996). Despite all efforts made, no obligate anaerobes were isolated in the present study.…”

“…Briefly, every agar culture was performed in duplicate using a rich agar (SCH) incubated in an anaerobe pouch system while thioglycolate was chosen as conventional enrichment broth because of its excellent recovery of anaerobes from human sterile body fluids (Reinhold et al . 1988; Scythes et al . 1996).…”

A retrospective analysis of antimicrobial resistance in pathogenicEscherichia coliandSalmonellaspp. isolates from poultry in Uganda

“…were archived at −30°C and −80°C. During the study, archived isolates were recovered as per the procedures by Scythes et al [ 18 ]. Briefly, the procedure involved first inoculating 200 µl of the bacteria stocks in 2 ml of brain heart infusion broth (Oxoid, United States of America) and incubation at 37°C for 24 hrs.…”

Bacteriological and mechanical impact of the Sterrad sterilization method on personalized 3D printed guides for mandibular reconstruction

“…Schaedler broth have been chosen for its ability to grow fastidious bacteria, including anaerobes, and its use in the diagnosis of prosthetic infections 26,27 . Moreover, the Schaedler broth also allows the culture of E. coli, P. aeruginosa, E. faecalis and S. aureus species, according to previous publications [28][29][30] . The preliminary study aiming to assess the sensitivity of Schaedler broths shows a good match between the theoretical number of bacteria inoculated and the proportion of positive schaedler broth with the lowest bacterial incolum.…”