The objective of this study was to determine the chemical composition of eight vine shoot waste (VSW) varieties (Sauvignon Blanc, Pinot Noir, Feteasca Regala, Busuioaca de Bohotin, Muscat Ottonel, Cabernet Sauvignon, Feteasca Neagra and Feteasca Alba) regarding cellulose, hemicellulose, lignin, ash, moisture, mineral, protein, elemental analysis for the production of bioethanol. The highest cellulose and hemicellulose content (almost 65%) suggests that the VSW varieties can be used as raw material for bioethanol production. The most abundant minerals found in the VSW samples were Sr, Fe and Mn, which are indicators for vineyard production in Romania. The autohydrolysis fractionation method was applied for the separation of cellulosic and hemicellulosic sugars from the VSW varieties.