Evaluation of osteoblast differentiation and function when cultured on mesoporous bioactive glass adsorbed with testosterone

Abstract

Mesoporous bioactive glass (MBG), a kind of porous materials with great osteoconductive and osteoinductive ability, shows promising application in bone tissue engineering due to its high specific surface area, orderly channel structure, and large pore volume. Here we reported that the proliferation, differentiation, and mineralization were promoted in MC3T3-E1 cells cultured on MBG which adsorbed with testosterone (MBG/T). We found that transcriptional activity of Runx2 which is a critical transcription factor is increased in MC3T3-E1 cells cultured on MBG/T. Intriguingly, we observed that ERK phosphorylation was enhanced in MC3T3-E1 cells cultured on MBG/T. We showed that activated Runx2 in MC3T3-E1 cells cultured on MBG/T is through Erk1/2 phosphorylation. Consistent with this result, we also found that the expression of osteoblastic marker genes were increased. Therefore, we concluded that osteoblast differentiation and mineralization was enhanced after cells cultured on MBG/T through Erk1/2-activated Runx2 pathway. Our findings provided that MBG/T is a potential material in the process of bone repair.

Polymeric and Composite Carriers of Protein and Non-Protein Biomolecules for Application in Bone Tissue Engineering

“…In order to obtain these scaffolds, the solutions of both components were thoroughly mixed and then freeze-dried. Sustained-release testosterone was confirmed by the results obtained [ 188 ]. A load-bearing biodegradable scaffold made of polypropylene fumarate/tricalcium phosphate composites was modified by adding testosterone, bone morphogenetic protein-2 (BMP-2), or their combination in order to promote bone regeneration.…”

Design of novel functionalized collagen-chitosan-MBG scaffolds for enhancing osteoblast differentiation in BMSCs

“…Besides, in our study, the advantages associated with MBG to serve as an important drug delivery system for the control-release of other biological factors were not well utilized. Our previous work has shown that the different biological factors, such as testosterone and epithelial growth factor, which can be absorbed by MBG could functionally control factor release to exhibit a long lasting effect on the process of osteoblast differentiation (Wang et al 2017, Gao et al 2018. Therefore, it will be quite promising to try different loading osteogenic factors with C-C-MBG scaffolds for the bone regeneration in our further studies.…”

“…MBG was synthesized through sol-gel and template self-assembly procedures as reported previously by our group (Gao et al 2018). Firstly, 4.8 g EO 20 PO 70 EO 20 (P123, Sigma, USA) was dissolved in 60 ml deionized water and the pH was adjusted to 1 by adding HCl.…”

Local delivery of a novel PTHrPviamesoporous bioactive glass scaffolds to improve bone regeneration in a rat posterolateral spinal fusion model

“…40 Such contradictory results may reect the fact that most previous studies employed the MC3T3-E1 osteoblast cell for the in vitro studies. 41 Unlike BMSCs, MC3T3-E1 osteoblast cells are a kind of osteoblast precursor cells. Additionally, in previous studies, a special osteoinductive culture medium (containing 10% fetal bovine serum, 50 mg mL À1 ascorbic acid, 10 mM b-glycerol-2phosphate, and 1% penicillin-streptomycin) was used to pretreat the cells to induce osteoblastic differentiation.…”