Evaluation of oxidative stress and antioxidant activity in pre and post hemodialysis in chronic renal failure patients from Western region of Nepal

Abstract

Objective: To evaluate the oxidative stress and antioxidant activity in pre and post hemodialysis in chronic renal failure patients using a diacetate cellulose membrane. Background: Chronic Renal Failure is a gradual, progressive and irreversible loss of normal functioning of kidneys. This is associated with increased formation of reactive oxygen species and increased oxidative stress. No studies have been undertaken in this area in Nepal. Method:The study groups were devided into chronic renal failure patients undergoing hemodialysis (n=52) and healthy controls (n=52). Diacetate cellulose was used as dialyzer membrane for chronic renal failure patients. Blood samples were collected in EDTA bottles and plasma was used for the estimation of oxidative stress marker, total antioxidant activity, vitamin C, α-tocopherol, urea and creatinine. Results: There was significant reduction in the levels of vitamin C,α -tocopherol and total antiox-idant activity in post dialysis patients compared to predialysis and control groups (p<0.001). Plasma urea and creatinine levels were high in predialysis patients compared to controls and was significantly reduced after dialysis. Significantly elevated malondialdehyde levels were found in chronic renal failure patients before and after dialysis when compared with controls. Conclusion:The results of our study indicate that there are reduced levels antioxidants in chronic renal patients in pre and post dialysis condition and also more loss of antioxidants after dialysis. All these condition leads to increased oxidative stress in these patients.

Estudio comparativo del estado nutricional y el estrés oxidativo en pacientes con hemodiális de alto flujo versus hemodiafiltración online

“…El desarrollo de la ateroesclerosis se ha relacionado con el aumento de la peroxidación lipídica en los pacientes con ERC. Estos cambios oxidativos reducen HDL, aumentan LDL y además producen la oxidación de LDL, la LDL oxidada parece ser un factor importante para el desarrollo de lesiones ateroscleróticas mediante la promoción de los daños y la destrucción directa de la célula endotelial (Negamma et al, 2014).…”