Evaluation of Oxidative Stress and Antioxidant Status in Chronic Obstructive Pulmonary Disease

Abstract

Chronic obstructive pulmonary disease (COPD) is a common respiratory condition involving the airways and characterized by airflow limitation. Gaseous and noxious particles play an important role in this process. Antioxidants are the substances that may protect cells from the damage caused by unstable molecules known as free radicals. The increased oxidative stress in patients with COPD is the result of an increased burden of inhaled oxidants, as well as increased amounts of reactive oxygen species (ROS) generated by various inflammatory, immune and epithelial cells of the airways. A total of 150 subjects with COPD and 100 healthy controls subjects were enrolled in this study from the period October 2015 to January 2016. The investigation included measurements of plasma superoxide dismutase activity (SOD), catalase activity (CAT), glutathione content (GSH) reduced form, (GPx) glutathione peroxidase, glutathione reductase (GR) and lipid peroxidation (LPO). Absorbance was measured by UV spectrophotometer. The estimated values of SOD, catalase, GPx, GSH and GR were found to be significantly (P = 0.0001) lower among the cases compared with controls. But, the levels of MDA were higher (P = 0.0001) in cases as compared to control group and there was significant difference in the oxidative stress parameters among the various stages of COPD. The post hoc analysis revealed that SOD was significantly (P < 0.01) lower among the mild, moderate and severe patients compared with very severe patients. The catalase was also observed to be significantly (P = 0.01) lower among mild, moderate and severe patients than very severe patients. The GPx was found to be significantly (P = 0.002) lower among the mild, moderate and severe patients compared with very severe patients. MDA was observed to be significantly higher in mild, moderate and severe patients compared with very severe (P = 0.001). GR was significantly (P = 0.003) lower among mild, moderate and severe patients than very severe patients. However, there was no significant difference in GSH among severity of COPD patients. This study suggests that oxidant and antioxidant imbalance plays an important role in various stages of severity of COPD. These results revealed the presence of an oxidative stress in subjects with COPD, and it is proportionate to the severity of disease.

Association of serum bilirubin level with lung function decline: a Korean community-based cohort study

“…In human lungs, it is reported that oxidant-antioxidant imbalance is associated with airways obstruction, and altered oxidant-antioxidant balance in patients with COPD increases in parallel with the severity of the disease [ 21 ]. Repine et al reported that lipid peroxidation causes damage to multiple cell membrane components and impairs cell structure and permeability [ 22 ].…”

“…Patients with COPD have higher levels of lipid peroxidation products in sputum [ 23 ], and serum levels of these products are higher in patients with severe airflow limitation compared with those with moderate limitation [ 24 ]. Based on these results, we suggest that bilirubin might protect the lungs by inhibiting lipid peroxidation [ 21 – 24 ].…”

Significance of thiol/disulphide homeostasis and ischemia modified albumin levels in chronic obstructive pulmonary disease

“…These include glutathione, glutathione peroxidase, superoxide dismutase, catalase, ferroxidase, and myeloperoxidase [43][44][45][46]. IMA is an oxidant marker resulting from the differentiation of albumin, with antioxidant properties, in situations involving increased oxidative stress [23].…”

Protective Effect of Jianpiyifei II Granule against Chronic Obstructive Pulmonary Disease via NF‐κB Signaling Pathway

“…Antioxidant enzymes can either facilitate antioxidant reactions or directly decompose ROS [ 49 ]. GSH-Px and CAT play significant roles in peroxyl scavenging mechanisms and in maintaining the functional integration of cell membranes [ 50 ]. In addition, as the main product of lipid peroxidation, MDA is closely associated with pulmonary dysfunction and considered a biomarker in the assessment of lipid peroxidation [ 51 ].…”