Evaluation of Oxidative Stress, Apoptosis, and Expression of MicroRNA-208a and MicroRNA-1 in Cardiovascular Patients

Abstract

Background: MicroRNA expression signature and reactive oxygen species (ROS) production have been associated with the development of cardiovascular diseases (CVDs). This study aimed to evaluate oxidative stress, inflammation, apoptosis, and the expression of miRNA-208a and miRNA-1 in cardiovascular patients. Methods: The study population included four types of patients (acute coronary syndromes (ACS), myocardial infarction (MI), arrhythmia, and heart failure (HF)), with 10 people in each group, as well as a control group. Quantitative real-time PCR was performed to measure mir-208 and miR-1 expression, the mRNAs of inflammatory mediators (TNFα, iNOS/eNOS), and apoptotic factors (Bax and Bcl2). XOX, MDA, and antioxidant enzymes (CAT, SOD, and GPx) were measured by ZellBio GmbH kits by an ELISA Reader. Results: The results showed significant decreases in the activity of antioxidant enzymes (CAT, SOD, and Gpx) and a significant increase in the activity of the MDA and XOX in cardiovascular patients. Significant increases in IL-10, iNos, iNOS / eNOS, and TNF-α in cardiovascular patients were also observed. Also, a significant increase in the expression of miR-208 (HF> arrhythmia> ACS> MI) and a significant decrease in the expression of miR-1 (ACS> arrhythmia> HF> MI) were found in all four groups in cardiovascular patients. Conclusions:The results showed increases in oxidative stress, inflammation, apoptotic factors, and in the expression of miR-208a in a variety of cardiovascular patients (ACS, MI, arrhythmia, and HF). It is suggested that future studies determine the relationships that miR-1, miR-208, and oxidative stress indices have with inflammation and apoptosis.

Crosstalk between microRNA and Oxidative Stress in Heart Failure: A Systematic Review

“…The lowest concentration of GPX and SOD along with highest expression of miRNA-208 activity was observed in the group of heart failure. miRNA-1 was significantly downregulated in HF patients in comparison to other groups [ 37 ].…”

Molecular Mechanisms of Notopterygii Rhizoma Et Radix for the Treatment of Arrhythmia Based on Network Pharmacology

“…In addition, the results of molecular docking also exhibited that all of them have good binding properties with core target genes. Clinical studies have shown that the expression of BAX was increased in cardiovascular patients, including arrhythmia [32], and caspase3 variants was susceptible to atrial brillation (AF). Myocardial apoptosis can cause electrical and structural cardiac remodeling [33]; and as the important apoptotic factors, the expression of CASP8, CASP9, BAX was altered in many cardiovascular disease (e.g.…”

MikroRNA w zaburzeniach rytmu serca

“…The overexpression of miR-208a in cardiovascular patients is proven to be associated with increased oxidative stress, inflammation, and apoptosis. A recent study showed a significant increase in the expression of miR-208a in heart failure, VA, and myocardial infarction patients when compared to the control group [30]. The increase in miR--208a was in pair with apoptotic and inflammation factors, like BAX and TNF-α, thus revealing miR-208a's potential role in these processes.…”