Evaluation of Oxidative Stress in Cardiomyocytes during the Aging Process in Rats Treated with Resveratrol

Abstract

The substantial increase in the number of elderly people in our societies represents a challenge for biology and medicine. The societies of the industrialized countries are subject to a progressive aging process that translates into an increase in the cardiovascular risk of the population. In the present work, the activity of catalase and superoxide dismutase was evaluated, as well as markers of oxidative stress (concentration of nitric oxide and total lipoperoxidation in its main components: malondialdehyde and 4-hydroxyalkene) in cardiomyocytes during the aging process in rats treated with resveratrol. Rats were divided into 4 groups according to the following categories: control (without treatment), negative control group (administered with physiological solution with 10% ethanol), positive control group (administered with vitamin E, 2 mg/kg/day), and group administered with resveratrol (10 mg/kg/day); these groups in turn were divided into 2, 4, 6, and 8 months of treatment. The analysis of nitric oxide showed a decreased level in the cardiac tissue in the groups treated with resveratrol; the same occurs when total lipoperoxidation is analyzed. The enzymatic activity studied (catalase and superoxide dismutase) did not present significant changes with respect to the controls. It is concluded that the cardioprotective effect of resveratrol is due to the antioxidant effect and other antiaging effects and not to the activation of the enzymes catalase and superoxide dismutase.

Resveratrol ameliorates brain injury via the TGF‑β‑mediated ERK signaling pathway in a rat model of cerebral hemorrhage

“…The rat model of cerebral hemorrhage was established using the modified ischemia/reperfusion method as previously described (10). Rats received right middle cerebral artery occlusion for 90 min and reperfusion by withdrawal of the filament at 37°C Immediately, rats with ischemia/reperfusion-induced cerebrovascular injury were randomly divided into two groups (each, n=8) and were administered an intravenous injection of resveratrol (10 mg/kg/day; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) or the same volume of PBS (serving as the control) as described previously (11). The treatments were continued for 20 days.…”

The Effects of Resveratrol on Telomeres and Post Myocardial Infarction Remodeling

“…An antioxidant-rich diet is therefore beneficial for preventing the effects of ROS in the cardiovascular system [ 39 ]. Studies by Aguilar-Alonso et al on oxidative stress markers (total lipoperoxidation and nitric oxide (NO) levels) as well as catalase activity and superoxide dismutase (SOD) in aging rat cardiomyocytes treated with resveratrol showed a decrease in NO Levels and total lipoperoxidation, but the activity of catalase and SOD was not significantly affected, and it was concluded that the anti-aging and cardioprotective properties of resveratrol are mediated mainly by its antioxidant effects [ 40 ].…”

Controversial Impact of Sirtuins in Chronic Non-Transmissible Diseases and Rehabilitation Medicine

“…The combination of Res and exercise has been found beneficial on vascular function reducing inflammatory markers and enhancing vessel responsiveness [ 66 ]. However, a recent work reported that the beneficial effect of resveratrol could be due to an antioxidant effect without any evidence of Sirt1 activation [ 67 ].…”