Evaluation of partial tef1, rpb2, and nLSU sequences for identification of isolates representing Armillaria calvescens and Armillaria gallica from northeastern North America

Brazee, Nicholas J.; Hulvey, Jon; Wick, Robert L.

doi:10.1016/j.funbio.2011.05.008

Cited by 25 publications

(25 citation statements)

References 37 publications

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“…Only Antonin et al (2009) used it for differentiation of A. gallica and A. cepistipes. Other studies using the EF-1 alpha gene included Armillaria from the northern and southern hemispheres (Maphosa et al 2006), Japan (Hasegawa et al 2010) and North America (Brazee et al 2011(Brazee et al , 2012Ross-Davis et al 2012). In our studies the EF-1 alpha gene analysis allowed recognition of A. solidipes (BS 99%) and clear separation of A. borealis from A. solidipes and A. cepistipes from A. gallica (BS 68% and 92%, respectively).…”

Section: Discussionmentioning

confidence: 52%

Populations of Armillaria species in pine plantations in west-central Poland

2015

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Two diploid isolates of Armillaria gallica and 143 diploid isolates of A. solidipes were obtained from 145 samples of rhizomorphs, fruit bodies and infected wood of sessile oak and Scots pines from 5-10-year-old Scots pine plantations in three Forest Districts located 100-350 km apart in west-central Poland. Based on pairings among the 145 isolates, 18 somatic compatibility groups (genets) of A. solidipes were distinguished in the three plantations. Sequencing of ITS1/2 rDNA of 18 isolates representing the 18 genets delineated four nuclear haplotypes. This suggests that many of the A. solidipes genets are closely related and were possibly established by sib-related basidiospores. With a few exceptions there was general geographical specialization of genets but not haplotypes. Geographical specialization of genets and the dominance of single haplotypes suggest that the A. solidipes population results from clonal rather than sexual reproduction. Sequencing of the ITS1/2 and IGS-1 rDNA showed small nucleotide diversity in ITS1/2 rDNA and much more diversity in IGS-1 rDNA of the isolates of A. solidipes studied. However, none of these regions has sufficient resolution for the clear differentiation of A. solidipes from A. borealis. The sequences of the EF 1-alpha gene showed high interspecific variability in Armillaria species and very low intraspecific variability in A. solidipes. This gene is the most appropriate for reliable identification of biological species and subgroups of Armillaria. Its application is the most useful in ecological and epidemiological studies of Armillaria.

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Section: Discussionmentioning

confidence: 52%

Populations of Armillaria species in pine plantations in west-central Poland

2015

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“…The subgroups of A. mellea have been documented with isolates from USA, Europe and Asia (Coetzee et al 2000;Baumgartner et al 2010;Brazee et al 2011), suggesting a process of speciation (Coetzee et al 2000). The subgroups of A. mellea have been documented with isolates from USA, Europe and Asia (Coetzee et al 2000;Baumgartner et al 2010;Brazee et al 2011), suggesting a process of speciation (Coetzee et al 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Even if they are found, the basidiomata of closely related species can be difficult to distinguish based on morphology (Kim et al 2006;Brazee et al 2011;Ross-Davis et al 2012). However, Armillaria spp.…”

Section: Introductionmentioning

confidence: 99%

Incidence and phylogenetic analyses of Armillaria spp. associated with root disease in peach orchards in the State of Mexico, Mexico

et al. 2013

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Incidence of peach [Prunus persica (L.) Batsch] tree mortality attributed to Armillaria root disease was assessed from 2009 to 2011 in 15 orchards in the State of Mexico, Mexico. Incidence increased gradually every year of assessment, reaching average values of 9.7, 15.3 and 20.3% tree mortality and 23.2, 24.7 and 28.3% disease-impacted area of the orchards during 2009, 2010 and 2011, respectively. The cultivars 'Nemaguard' and 'Criollo of La Goleta', a local rootstock used in the region, were both susceptible to the disease. To identify species of Armillaria isolated from infected peach trees, two nuclear rDNA regions (partial 5.8S-ITS2-LSU D-domains and partial 3′ LSU-IGS1) and the translation elongation factor-1a (tef-1a) gene were sequenced and compared with sequences of known Armillaria species. DNA sequence analysis from 49 Armillaria isolates revealed that five isolates (10.2%) were Armillaria mellea and eight isolates (16.3%) were Armillaria gallica. DNA sequences from the remaining 36 isolates (73.5%) showed no close similarity to Armillaria sequences in GenBank, and apparently represent an undescribed Armillaria species. This undescribed species was the most widely distributed in the region of study. Separate phylogenetic analyses of the LSU region (D1-D3 domains concatenated with the partial 3′ end) and the tef-1a region show that the undescribed species is quite distinct from other Armillaria spp. reported in North America.

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“…Identifying this genus based on morphological traits is complicated because, in most cases, only asexual or vegetative structures are found. One of the issues extensively discussed in the literature is the lack of sexual structures that would allow identifying the genus based on classical taxonomy Brazee et al, 2011;Ross-Davis et al, 2012). Over the past few years, molecular data, (SAGARPA, 2014).…”

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confidence: 99%

“…El género Armillaria incluye más de 40 especies, su identificación a partir de los caracteres morfológicos es complicada, debido a que en la mayoría de los casos, solo se encuentran estructuras asexuales o vegetativas, uno de los puntos que se ha discutido ampliamente en la literatura es la falta de estructuras sexuales para realizar la identificación taxonómica clásica Brazee et al, 2011;Ross-Davis et al, 2012). En los últimos años, los datos moleculares, específi-camente las secuencias de ADN, se están utilizado para identificar las especies de hongos y realizar estudios filogenéticos (Cai et al, 2011;Guo et al, 2016;Koch et al, 2017).…”

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