Evaluation of PC12 Cells’ Proliferation, Adhesion and Migration with the Use of an Extracellular Matrix (CorMatrix) for Application in Neural Tissue Engineering

Abstract

The use of extracellular matrix (ECM) biomaterials for soft tissue repair has proved extremely successful in animal models and in some clinical settings. The aim of the study was to investigate the effect of the commercially obtained CorMatrix bioscaffold on the viability, proliferation and migration of rat pheochromocytoma cell line PC12. PC12 cells were plated directly onto a CorMatrix flake or the well surface of a 12-well plate and cultured in RPMI-1640 medium and a medium supplemented with the nerve growth factor (NGF). The surface of the culture plates was modified with collagen type I (Col I). The number of PC12 cells was counted at four time points and then analysed for apoptosis using a staining kit containing annexin V conjugate with fluorescein and propidium iodide (PI). The effect of CorMatrix bioscaffold on the proliferation and migration of PC12 cells was tested by staining the cells with Hoechst 33258 solution for analysis using fluorescence microscopy. The research showed that the percentage of apoptotic and necrotic cells was low (less than 7%). CorMatrix stimulates the proliferation and possibly migration of PC12 cells that populate all levels of the three-dimensional architecture of the biomaterial. Further research on the mechanical and biochemical capabilities of CorMatrix offers prospects for the use of this material in neuro-regenerative applications.

Biological Scaffolds for Congenital Heart Disease

“…ProxiCor ® (previously known under the commercial name of CorMatrix ® ) is a commercially available decellularised porcine SIS scaffold [2,[106][107][108]. It has gained great attention in the cardiovascular field [109] since 2010 due to its ability to support the ingrowth of host cells, promote cell proliferation and differentiation, contractility, absorbability, neovascularisation, and a lack of immunogenicity [107]. Furthermore, it can grow, repair, and remodel congenital heart and vascular defects [110,111], facilitate pericardium reconstruction [112], valve reconstruction in adults and children [113], endocardium reconstruction, and the repair of post-myocardial infarction defects [114] through the promotion of native tissue growth without the need for repeated surgeries [115].…”

Correction: Gębczak et al. Evaluation of PC12 Cells’ Proliferation, Adhesion and Migration with the Use of an Extracellular Matrix (CorMatrix) for Application in Neural Tissue Engineering. Materials 2021, 14, 3858

“…In the original publication [ 1 ], the funder National Science Centre, Poland , grant no. DEC-2012/06/M/NZ4/00138 granted to Wojciech Fortuna was not included.…”