Evaluation of periodontal pathogens of the mandibular third molar pericoronitis by using real time PCR

Şençimen, Metin; Saygun, Işıl; Gülses, Aydın; Bal, Vehbi; Açıkel, Cengiz Han; Kubar, Ayhan

doi:10.1111/idj.12109

Cited by 16 publications

(14 citation statements)

References 25 publications

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“…Our results demonstrated that the distal subgingival plaque of M3M was most likely to be associated with the pathogenesis of acute pericoronitis. The result was consistent with previous studies (Rajasuo et al, 2007;Sencimen et al, 2014). More than clinical experience, we presented the evidence that microbial community changes of subgingival plaque were more remarkable than the other two susceptive sites.…”

Section: Discussionsupporting

confidence: 93%

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Microbial Profile During Pericoronitis and Microbiota Shift After Treatment

Huang

Zheng

et al. 2020

Front. Microbiol.

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The microflora of the distal pocket is considered as the major cause of pericoronitis. How the oral microflora changes during pericoronitis and whether different types of impacted third molar harbor the same microflora are still unknown. Saliva, subgingival plaque, and gingival plaque of mandibular third molars (M3Ms) were collected from twelve patients with acute pericoronitis. They were given local irrigation or local irrigation + antibiotics according to symptoms. Samples were harvested at the first visit with pericoronitis, 1 week after treatment, and 6 weeks after treatment. 16S rRNA gene polymerase chain reaction products were generated and sequenced after DNA isolation. Comparison of three sampling sites showed that, the subgingival plaque of M3Ms had most remarkable changes in symptomatic period, including a significant increase in microbial richness, and a convergent trend in microbial composition. After treatment, the subgingival microbiome was altered and largely returned to the state in asymptomatic period. In summary, the distal subgingival microbiota of M3M was most likely to be associated with the pathogenesis of pericoronitis. The post-treatment microbiota shift of M3M proved the effectiveness of treatment. The inclination type of impacted M3Ms and treatment method would also make a difference to the pericoronal microbiota.

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Section: Discussionsupporting

confidence: 93%

“…Numerous studies have demonstrated that the microbial flora associated with pericoronitis is mixed and dominated by anaerobic bacteria (Leung et al, 1993;Rajasuo et al, 2007;Sencimen et al, 2014). They suggested a number of possible pathogens, including Streptococcus, Staphylococcus, and Fusobacterium species, etc.…”

Section: Introductionmentioning

confidence: 99%

Microbial Profile During Pericoronitis and Microbiota Shift After Treatment

Huang

Zheng

et al. 2020

Front. Microbiol.

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“…Full‐mouth tooth extraction is reported to significantly change the oral cavity microflora . Nevertheless, periodontal pathogens in chronic periodontitis are reported to be the nonstreptococcus species, unlike the findings by Teymoortash et al…”

Section: Discussionmentioning

confidence: 81%

A population‐based study on the association between chronic periodontitis and sialolithiasis

et al. 2016

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“…Inflammation is strictly regulated by the body where inadequate inflammatory processes can cause damage or persistent infection, while excessive inflammation potentially results in chronic or systemic inflammatory disease. [21][22][23][24] COX)converts arachidonic acid into prostaglandin-H2. Two forms of COX have been identified, namely; COX-1 which is expressed constitutively and COX-2 which is expressed due to growth factors, oncogenes, cytokines and endotoxins.…”

Section: Discussionmentioning

confidence: 99%

The role of COX-2, caspase-1 and IL-17 in pericoronitis-related inflammation due to lower third molar impaction

Prayitno

2019

Dent. J.

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Background: Inflammation of the pericorona due to lower third molar impaction (LTMI) is often diagnosed as pericoronitis. Expression of cyclooxigenase-2 (COX-2) and caspase-1 may be induced by lipopolysacharide (LPS) and cause pyroptosis with minimal inflammation. When LPS activates toll-like receptor (TLR-4), NOD-like receptors containing domain pyrin 3 (NLRP3) inflammasome will activate the release of pro-caspase-1 to caspase-1, followed by the secretion of interleukin (IL)-1β. IL-1β and IL-23 which induces CD4 + Tcells (Th17) to produce IL-17 as a pro-inflammation cytokine. Purpose: This study aimed to identify the respective roles of COX2, caspase-1 and IL-17 in pericoronitis inflammation of the pericorona due to LTMI. Methods: Frozen section samples were produced through LTMI pericorona tissue biopsy using material provided by the Dental and Oral Clinic at Muwardi Hospital, Surakarta. The paraffin block produced was subsequently cut using a clean microtome with the resulting thin slices being placed on an object glass coated with polylysine. A diagnosis of pericoronitis was subsequently made by a pathologist. Immunohistochemical staining for COX-2, caspase-1 and IL-17 was carried out by indirect tyramide signal amplification (TSA) method. Photos were obtained by means of 100X, 200X, 400X and 1000X objective lensed microscopes to qualitatively assess the above mentioned protein expressions. T-Test was conducted in order to establish the difference in expression between the control group and pericoronitis due to LTMI. Results: The presence of a brownish yellow color indicated the expression of COX-2, caspase-1 and IL-17 in pericorona epithelial cells which visible expression categorized as moderate (30-70%). The mean expression of COX-2, caspase-1 and IL-17 was categorized as mild and there was no significant difference between the expression of the three proteins. Conclusion: COX-2, caspase-1 and IL-17 play an important role in the phyroptosis signal of LTMI pericoronitis in cases of low inflammation.

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Evaluation of periodontal pathogens of the mandibular third molar pericoronitis by using real time PCR

Abstract: Tf plays an important role in the development of clinical symptoms related to pericoronitis.

Cited by 16 publications

References 25 publications

Microbial Profile During Pericoronitis and Microbiota Shift After Treatment

Microbial Profile During Pericoronitis and Microbiota Shift After Treatment

A population‐based study on the association between chronic periodontitis and sialolithiasis

The role of COX-2, caspase-1 and IL-17 in pericoronitis-related inflammation due to lower third molar impaction

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