Evaluation of pH at the Bacteria–Dental Cement Interface

Abstract

Physiochemical assessment of the parasite-biomaterial interface is essential in the development of new biomaterials. The purpose of this study was to develop a method to evaluate pH at the bacteriadental cement interface and to demonstrate physiochemical interaction at the interface. The experimental apparatus with a well (4.0 mm in diameter and 2.0 mm deep) was made of polymethyl methacrylate with dental cement or polymethyl methacrylate (control) at the bottom. Three representative dental cements (glass-ionomer, zinc phosphate, and zinc oxide-eugenol cements) were used. Each specimen was immersed in 2 mM potassium phosphate buffer for 10 min, 24 hrs, 1 wk, or 4 wks. The well was packed with Streptococcus mutans NCTC 10449, and a miniature pH electrode was placed at the interface between bacterial cells and dental cement. The pH was monitored after the addition of 1% glucose, and the fluoride contained in the cells was quantified. Glass-ionomer cement inhibited the bacteria-induced pH fall significantly compared with polymethyl methacrylate (control) at the interface (10 min, 5.16 ± 0.19 vs. 4.50 ± 0.07; 24 hrs, 5.20 ± 0.07 vs. 4.59 ± 0.11; 1 wk, 5.34 ± 0.14 vs. 4.57 ± 0.11; and 4 wks, 4.95 ± 0.27 vs. 4.40 ± 0.14), probably due to the fluoride released from the cement. This method could be useful for the assessment of pH at the parasite-biomaterial interface.

Influence of S. mutans on Base-metal Dental Casting Alloy Toxicity

“…Dental caries, the most prevalent bacterial disease (Berkowitz, 2003), is primarily caused by colonization of the oral cavity by acidogenic S. mutans (Mayanagi et al, 2011). S. mutans, a lactic-acid-producer, has the capability to reduce intra-oral pH levels during and following the ingestion of foods and liquids (Aamdal-Scheie et al, 1996), resulting in demineralization and subsequent loss of enamel and dentin (Takahashi and Nyvad, 2011).…”

Salivary Indicators for a Healthy Mouth: An In Vivo Study

“…Glass ionomer cement have the ability to neutralize the salivary acid by buffering the lactic acid via the release of chemical ions and also has an antibacterial effect (Mayanagi et al, 2011).…”

“…The systematic removal of all carious lesions and a thorough prophylaxis are sometimes considered to be effective methods for bringing the disease under control (Mayanagi et al, 2011;Subramanyam et al, 2016;I. Nedeljkovic et al, 2016).…”

Detection of Micrococcus Luteus Biofilm Formation in Microfluidic Environments by pH Measurement Using an Ion-Sensitive Field-Effect Transistor

“…The tip diameter of the present ISFET is 0.5 mm, drastically smaller than that of a glass electrode, and thus reduces the quantity of solution required for measurement [12]. ISFETs have been applied to the culture medium of adherent mammalian cells [11–14], sea urchin embryos [15], and bacteria [8,16–18] in order to analyze metabolism of these organisms. Moreover, ISFETs have been adapted by fabricating microtanks from poly(dimethylsiloxane) [16,17].…”