Evaluation of plasmatic MMP-8, MMP-9, TIMP-1 and MPO levels in obese and lean women

Andrade, Vanessa L.; Petruceli, Eveline; Belo, Vanessa A.; Andrade-Fernandes, Claudia M.; Russi, Cristiane V. Caetano; Bosco, Adriana; Tanus‐Santos, José Eduardo; Sandrim, Valéria C.

doi:10.1016/j.clinbiochem.2012.01.008

Cited by 60 publications

(59 citation statements)

References 32 publications

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“…Inhibition of MMP-9 activation interrupted macrophage migration to the elicited site of inflammation [8]. MMP-9 is also reported to play an important role in obesity-mediated adipose tissue remodelling and obese individuals are known to have increased MMP-9 levels [9]. MMP-9 expression is typically absent or minimal in normal tissues and is greatly enhanced during inflammation and wound healing [10].…”

Section: Introductionmentioning

confidence: 99%

FSL-1 Induces MMP-9 Production through TLR-2 and NF-κB /AP-1 Signaling Pathways in Monocytic THP-1 Cells

Ahmad

Shihab

Jasem

et al. 2014

Cell Physiol Biochem

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Background: Matrix metalloproteinase-9 (MMP-9) is known to be implicated in the pathogenesis of many inflammatory disorders. FSL-1 (fibroblast-stimulating lipopeptide-1) induces cytokine production by monocytes/macrophages. However, it is unclear whether FSL-1 is also able to induce MMP-9 production. Herein, we determined whether FSL-1 could induce MMP-9 production, and if so, which signal transduction pathway(s) were involved. Methods: MMP-9 expression was assessed with real-time qPCR and ELISA. Signaling pathways were studied by using THP1-XBlue™ cells, THP1-XBlue™-defMyD cells, anti-TLR2 mAb and pharmacological inhibitors. Phospho and total proteins were determined by Western blotting. Results: FSL-1 induces MMP-9 expression (P<0.001) at both mRNA and protein levels in human monocytic THP-1 cells. Elevated activity (P<0.001) of NF-κB/AP-1 was also observed in FSL-1-treated THP-1 cells. FSL-1-induced MMP-9 secretion was markedly suppressed either by neutralizing anti-TLR-2 antibody or by inhibiting clathrin-dependent endocytosis. Furthermore, MyD88^-/- THP-1 cells did not express MMP-9 in response to FSL-1 treatment. By small interfering RNA-mediated knockdown, we also show that FSL-1-induced up-regulation of MMP-9 requires MyD88. Pre-treatment of THP-1 cells with inhibitors of JNK (SP600125), MEK/ERK (U0126; PD98056; XMD 8-92), p38 MAPK (SB203580) and NF-κB (BAY11-7085, Triptolide, Resveratrol) significantly suppressed (P<0.05) MMP-9 gene expression and NF-κB/AP-1 transcription factors activity. Conclusion: These findings provide the first evidence that FSL-1 induces TLR-2-dependent MMP-9 gene expression which requires the recruitment of MyD88 and leads to activation of MEK1/2 /ERK 1/2, MEK5/ERK5, JNK, p38 MAPK and NF-κB/AP-1.

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Section: Introductionmentioning

confidence: 99%

FSL-1 Induces MMP-9 Production through TLR-2 and NF-κB /AP-1 Signaling Pathways in Monocytic THP-1 Cells

Ahmad

Shihab

Jasem

et al. 2014

Cell Physiol Biochem

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“…Similar results were reported in an study conducted by Urkava et al [10] where oxidative stress was found to activate the development of IR in men. Andrade et al [12] also reported increased MPO activity in obese women and Heinecke et al [5] proposes MPO as a mediator of IR.…”

Section: Discussionmentioning

confidence: 99%

“…There is limited data relating inflammation [7][8][9] and oxidative stress [10][11][12] to IR in IFG [13]. Hence, in the present study we measured serum concentrations of IL-6 and MPO, to test the assumption that elevated levels of inflammatory and oxidative stress markers are linked with IR in individuals with IFG.…”

Section: Introductionmentioning

confidence: 99%

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Association of Interleukin-6 and Myeloperoxidase with Insulin Resistance in Impaired Fasting Glucose Subjects

et al. 2016

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Impaired fasting glucose (IFG) is a high risk subclinical condition for the progression of type 2 diabetes mellitus and the hyperglycemia seen in this condition is because of the development of insulin resistance (IR). Obesity, inflammation, oxidative stress and many other factors have been implicated in development of IR in type 2 diabetes mellitus and its successive complications. Current study was aimed to ascertain the correlation of inflammation and oxidative stress markers [interleukin-6 (IL-6) and myeloperoxidase (MPO)] with IR in subjects with IFG. In this study, 80 subjects (40 IFG, 40 healthy controls) aged 25-45 years were selected based on their fasting plasma glucose (FPG) values and clinical history. Serum insulin, IL-6 and MPO were estimated by ELISA method and IR was calculated using Homeostatic Model Assessment Index 2 (HOMA 2) calculator. Pearson's correlation coefficient and independent sample 't' test were used for statistical analysis. IL-6 and MPO were found to be significantly elevated in IFG group and both correlates significantly with IR (r 0.413, r 0.645). Only MPO had significant correlation with FPG (r 0.388). In conclusion, the association of altered levels of IL-6 and MPO with IR are suggestive of a role of inflammation and oxidative stress in the initiation and progression of IR in individuals with IFG.

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“…Ovi rezultati su u skladu sa rezultatima Zur-a i saradnika [15], koji su prikazali značajno veću aktivnost mijeloperoksidaze kod gojaznih osoba u poređenju sa ispitanicima sa optimalnom telesnom teži-nom (p<0.001). U skladu su i sa rezultatima studije Andrade-a i saradnika [16], čije se istraživanje odnosilo na ispitanice ženskog pola, gde je značajan porast aktivnosti mijeloperoksidaze izmeren kod gojaznih u odnosu na normalno uhranjene žene (p<0.05). Studija Olza-a i saradnika [17], tokom koje su ispitivana gojazna i normalno uhranjena deca, pokazala je da mijeloperoksidaza može biti rani marker povezan sa rizikom od razvoja kardiovaskularnih bolesti kod gojazne dece u periodu pre puberteta.…”

Section: Diskusijaunclassified

Examination of myeloperoxidase activity, as an indicator of inflammation in obese participants with metabolic syndrome

Matic¹,

Puhalo-Sladoje²,

Kisić³

et al. 2016

Praxis Med

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Gojaznost humanog organizma je složen metabolički poremećaj koji predstavlja jedan od najčešćih savremenih zdravstvenih problema. Brojna istraživanja ukazuju na udruženost hronične inflamacije niskog stepena i gojaznosti. Mijeloperoksidaza (MPO) i njeni reaktivni oksidanti učestvuju u oštećenju tkiva tokom inflamatornih procesa u humanom organizmu. Cilj istraživanja bilo je ispitivanje aktivnosti enzima mijeloperoksidaze u serumu gojaznih i ispitanika sa metaboličkim sindromom i testiranje povezanosti aktivnosti MPO i drugih pokazatelja zapaljenja u krvi ispitanika. Ispitanici su na osnovu antropometrijskih parametara i biohemijskih pokazatelja podeljeni u tri grupe: normalno uhranjeni (n=30), ispitanici sa abdominalnom gojaznošću (n=30) i ispitanici sa metaboličkim sindromom (n=30). U serumu ispitanika određivana je hlorinišuća aktivnost MPO spektrofotometrijski. Tokom ispitivanja utvrđene su značajne razlike u aktivnosti MPO kod sve tri grupe ispitanika i to najveća aktivnost izmerena je kod ispitanika sa metaboličkim sindromom (p<0.001). Utvrđena je pozitivna korelacija između aktivnost MPO i aterogenog indeksa, kao i između MPO i koncentracije LDL-holesterola, dok je negativna povezanost utvrđena između MPO i koncentracije HDL-holesterola. Ispitivanje je pokazalo da aktivnost MPO progresivno raste sa gojaznošću i metaboličkim sindromom. Dobijeni rezultati ukazju da MPO može biti od značaja u patofiziološkim mehanizmima koji dovode do razvoja komplikacija kod gojaznosti i metaboličkog sindroma.Ključne reči: mijeloperoksidaza, metabolički sindrom, inflamacija, gojaznost, dislipidemija. UVODGojaznost je složen metabolički poremećaj koji predstavlja jedan od najčešćih savremenih zdravstvenih problema. Prema definiciji Svetske zdravstvene organizacije gojaznost karakteriše indeks telesne mase (engl. Body Mass Index, BMI) preko 30 kg/m2 [1]. Gojaznost je povezana sa razvojem najčešćih savremenih humanih bolesti, kao što su koronarna srčana bolest, hipertenzija, diabetes mellitus tip 2 i dr. Brojna istraživanja ukazuju na udruženost perzistentne hronične inflamacije niskog stepena i gojaznosti [2]. Masno tkivo funkcioniše i kao endokrini organ, zbog sposobnosti adipocita da produkuju brojne biološki aktivne supstance -adipocitokine. Povećanje broja adipocita u organizmu gojaznih osoba dovodi do veće produkcije adipokina i pogoduje razvoju metaboličkog sindroma [3]. Metabolički sindrom karakteriše prisustvo više povezanih metaboličkih poremećaja u humanom organizmu, poremećaj regulacije metabolizma lipida (dislipidemija), hiperglikemija, neosetljivost na insulin, povećan krvni pritisak i abdominalna (centralna) gojaznost. Pomenuti metabolički poremećaji, kao i kod gojaznosti, predstavljaju značajne faktore rizika za pojavu brojnih komplikacija. Mijeloperoksidaza (MPO; E.C. 1.11.1.7.) jedan je od najzastupljenijih proteina u humanim neutrofilnim leukocitima i manje zastupljen u monocitima i tkivnim makrofagima [4]. Mijeloperoksidaza koja se nalazi u azurofilnim granulama neutrofila u toku aktivacije prelazi u fagocit...

show abstract

Evaluation of plasmatic MMP-8, MMP-9, TIMP-1 and MPO levels in obese and lean women

Cited by 60 publications

References 32 publications

FSL-1 Induces MMP-9 Production through TLR-2 and NF-κB /AP-1 Signaling Pathways in Monocytic THP-1 Cells

FSL-1 Induces MMP-9 Production through TLR-2 and NF-κB /AP-1 Signaling Pathways in Monocytic THP-1 Cells

Association of Interleukin-6 and Myeloperoxidase with Insulin Resistance in Impaired Fasting Glucose Subjects

Examination of myeloperoxidase activity, as an indicator of inflammation in obese participants with metabolic syndrome

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Evaluation of plasmatic MMP-8, MMP-9, TIMP-1 and MPO levels in obese and lean women

Cited by 60 publications

References 32 publications

FSL-1 Induces MMP-9 Production through TLR-2 and NF-&#954;B /AP-1 Signaling Pathways in Monocytic THP-1 Cells

FSL-1 Induces MMP-9 Production through TLR-2 and NF-&#954;B /AP-1 Signaling Pathways in Monocytic THP-1 Cells

Association of Interleukin-6 and Myeloperoxidase with Insulin Resistance in Impaired Fasting Glucose Subjects

Examination of myeloperoxidase activity, as an indicator of inflammation in obese participants with metabolic syndrome

Contact Info

Product

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FSL-1 Induces MMP-9 Production through TLR-2 and NF-κB /AP-1 Signaling Pathways in Monocytic THP-1 Cells

FSL-1 Induces MMP-9 Production through TLR-2 and NF-κB /AP-1 Signaling Pathways in Monocytic THP-1 Cells