Evaluation of Plasmodium vivax isolates in Thailand using polymorphic markers Plasmodium merozoite surface protein (PvMSP) 1 and PvMSP3

Suphakhonchuwong, Nutnicha; Chai่jaroenkul, Wanna; Rungsihirunrat, Kanchana; Na‐Bangchang, Kesara; Kuesap, Jiraporn

doi:10.1007/s00436-018-6106-1

Cited by 10 publications

(5 citation statements)

References 51 publications

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“…In this study, three distinct PCR-based types (A, B, and C) were observed. This result is consistent with the allelic variants observed in western and southern Thailand [4], the China-Myanmar border [33], and India [34], and a different band size designated as type D was reported in Pakistan [35]. In Hainan province, China, only types A and B were found [36].…”

Section: Discussionsupporting

confidence: 89%

“…About 90% of malaria cases in Thailand are caused by P. vivax [3]. In recent years, the proportion of P. vivax malaria cases in the districts or towns of Thailand that border Myanmar, Malaysia, Cambodia, and Laos has increased [4]. Malaria control depends mostly on antimalarial drugs and insecticides [5], and vaccines are seen as a possible additional way to fight malaria [6].…”

Section: Introductionmentioning

confidence: 99%

“…Of these, PvMSP-3α and PvMSP-3β are highly polymorphic and are regarded as potential vaccine candidates [17]. Understanding their genetic diversity is therefore important, because variations in candidate vaccine antigens can hamper their efficacy [4]. High levels of genetic diversity at these loci have been reported from diverse geographic areas of Thailand; 33 and 20 alleles of PvMSP-3α and 28 and 13 alleles of PvMSP-3β have been reported from the Thai-Myanmar border and the western region of Thailand, respectively [4,18].…”

Section: Introductionmentioning

confidence: 99%

“…Understanding their genetic diversity is therefore important, because variations in candidate vaccine antigens can hamper their efficacy [4]. High levels of genetic diversity at these loci have been reported from diverse geographic areas of Thailand; 33 and 20 alleles of PvMSP-3α and 28 and 13 alleles of PvMSP-3β have been reported from the Thai-Myanmar border and the western region of Thailand, respectively [4,18]. Therefore, understanding the genetic diversity of Thai P. vivax, which has been circulating in the country over time, can provide evidence for the association of certain genotypes with disease epidemiology and pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity of Plasmodium vivax Field Isolates from the Thai–Myanmar Border during the Period of 2006–2016

2023

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High levels of genetic variants of Plasmodium vivax have previously been reported in Thailand. Circumsporozoite surface protein (CSP), merozoite surface protein (MSP), and microsatellite markers were used to determine the genetic polymorphisms of P. vivax. This study aimed to investigate the molecular epidemiology of P. vivax populations at the Thai–Myanmar border by genotyping the PvCSP, PvMSP-3α, and PvMSP-3β genes. Four hundred and forty P. vivax clinical isolates were collected from the Mae Sot and Sai Yok districts from 2006–2007 and 2014–2016. Polymerase chain reaction with restriction fragment length polymorphism (RFLP) was used to investigate the genetic polymorphisms of the target genes. Based on PCR band size variations, 14 different PvCSP alleles were identified: eight for VK210 and six for VK247. The VK210 genotype was the dominant variant during both sample collection periods. Based on PCR genotyping, three distinct types (A, B, and C) for both PvMSP-3α and PvMSP-3β were observed. Following RFLP, 28 and 14 allelic variants of PvMSP-3α and 36 and 20 allelic variants of PvMSP-3β with varying frequencies were identified during the first and second periods, respectively. High genetic variants of PvMSP-3 and PvCSP were found in the study area. PvMSP-3β exhibited a higher level of genetic diversity and multiple-genotype infection versus PvMSP-3α.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity of Plasmodium vivax Field Isolates from the Thai–Myanmar Border during the Period of 2006–2016

2023

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“…The three polymorphic regions of PvMSP-1 gene, namely F1, F2 and F3 were analyzed according to the previously described method with slight modification (Imwong et al, 2005;Suphakhonchuwong et al, 2018). Total volume of PCR mixture was 20 µl, containing 12.5 µl of 2x PCR mixture (Intron, Singapore), 5 µl of DNA template and 20 pmol of each primer.…”

Section: Amplification Of Pvmsp1 Genementioning

confidence: 99%

THE USE OF ARCHIVED GIEMSA-STAINED BLOOD SMEARS AND RDT FOR PCR-BASED GENOTYPING OF Plasmodium vivax MEROZOITE SURFACE PROTEIN-1 IN CENTRAL KALIMANTAN PROVINCE, INDONESIA: PCR-Based Genotyping of Pvmsp-1 in Central Kalimantan Province, Indonesia

Lestarisa

Arwati

Dachlan

et al. 2021

AJID

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Background: Plasmodium vivax is transmitted most across the country of Indonesia. The country has set out a malaria elimination program by 2030. The information on genetic diversity of malarial parasites relates to malaria transmission in an endemic area may provide the information that can help the malaria control program to achieve the target. Therefore, the purpose of this study was to determine the genetic diversity of the Pvmsp-1 gene in Central Kalimantan Province. Materials and Methods: Samples were 140 of archived Giemsa-stained blood smear and rapid detection test. Samples were divided into the indigenous and migrant populations. After confirmation by single-step PCR, only P. vivax and mixed infection samples were amplified to nested PCR for genotyping of Pvmsp-1 allelic variation in segments F1, F2, and F3. Results: Genotyping of 23 PCR positive samples resulted in 13 genotypes. In segment F1, three allelic variants type A containing subtype A1 (1,050 bp), A2 (350 bp), A3 (150 bp), and type B (100 bp). In segment F2, mono genotypes were detected as variant type A (1,050 bp) and type B3 (150 bp), multiple genotypes were detected as type B containing subtype B1 (250 bp), B2 (200 bp), and B3 (150bp). In segment F3, three allelic variants generated from four mono genotypes were type A (350 bp), type B (300 bp), and two type C (250 bp). Conclusion: The low allelic variation of Pvmsp-1 gene may reflect the actual situation of the low malaria endemic status of the study sites.

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Genetic diversity of Plasmodium vivax and Plasmodium falciparum field isolates from Honduras in the malaria elimination phase

Zamora,

Pinto,

Escobar

et al. 2025

Current Research in Parasitology & Vector-Borne Diseases

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Evaluation of Plasmodium vivax isolates in Thailand using polymorphic markers Plasmodium merozoite surface protein (PvMSP) 1 and PvMSP3

Cited by 10 publications

References 51 publications

Genetic Diversity of Plasmodium vivax Field Isolates from the Thai–Myanmar Border during the Period of 2006–2016

Genetic Diversity of Plasmodium vivax Field Isolates from the Thai–Myanmar Border during the Period of 2006–2016

THE USE OF ARCHIVED GIEMSA-STAINED BLOOD SMEARS AND RDT FOR PCR-BASED GENOTYPING OF Plasmodium vivax MEROZOITE SURFACE PROTEIN-1 IN CENTRAL KALIMANTAN PROVINCE, INDONESIA: PCR-Based Genotyping of Pvmsp-1 in Central Kalimantan Province, Indonesia

Genetic diversity of Plasmodium vivax and Plasmodium falciparum field isolates from Honduras in the malaria elimination phase

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