Evaluation of polymerase chain reaction methods for detection of murine Helicobacter in nine diagnostic laboratories

Pathogenic microbial detection and control in laboratory animal facilities is essential to guarantee animal welfare, data validity and reproducibility. Helicobacter spp. are known to affect mice health, what may interfere with experimental outcomes. This study aimed to screen for Helicobacter spp. in mice from animal facilities in Rio de Janeiro, Brazil using a PCR-based method. Primers designed to specifi cally identify Helicobacter spp. were used to amplify feces or intestine DNA extracted of mice from four different animal facilities. The expected 375 base pairs (bp) amplicon was purifi ed, sequenced and a similarity of 95% was observed when compared to deposited sequences of H. hepaticus and H. bilis. In our screening, Helicobacter spp. was detected in ~59% of fecal and ~70% of intestine samples. Our study is the fi rst to screen for Helicobacter spp. in mouse facilities of a Rio de Janeiro University using a low cost, rapid molecular diagnostic test. Although Helicobacter spp. screening is not mandatory according to Brazilian animal welfare regulation it is recommended by institutional animal health monitoring programs guidelines worldwide, including ARRIVE, AAALAC and FELASA.

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PCR-based detection of Helicobacter spp. in animal facilities of a University in Rio de Janeiro, Brazil

Matos-Rodrigues

Masseron

Silva

et al. 2020

An. Acad. Bras. Ciênc.

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Evaluation of polymerase chain reaction methods for detection of murine Helicobacter in nine diagnostic laboratories

Cited by 1 publication

References 25 publications

PCR-based detection of Helicobacter spp. in animal facilities of a University in Rio de Janeiro, Brazil

PCR-based detection of Helicobacter spp. in animal facilities of a University in Rio de Janeiro, Brazil

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