Evaluation of polymerase chain reaction using protein b primers for rapid diagnosis of tuberculous meningitis

Sharma, Kusum; Sharma, Aman; Singh, Maninder Pal; Ray, Pallab; Dandora, Ritika; Sharma, Shiv K.; Modi, Manish; Prabhakar, Sudesh; Sharma, Mukut

doi:10.4103/0028-3886.72189

Cited by 31 publications

(28 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DNA extraction [16] DNA was extracted according to the CTAB-phenol-chloroform extraction method. Briefly, 0.2 ml of CSF was centrifuged at 10,000 rpm for 10 min.…”

Section: Processing Of Csf Samplementioning

confidence: 99%

“…MPB64 primer has shown good sensitivity for diagnosis of CNS TB [15]. Protein b was chosen as the third target specific for MTB complex because in our previous experience it was found to have good sensitivity and specificity in diagnosis of TBM patients [16]. To the best of our knowledge, the use of multiplex PCR assay using these three targets has not been evaluated in TBM.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the present study was undertaken to explore the utility of multiplex PCR using IS6110, protein b and MPB64 genes as targets for rapid diagnosis of TBM. We have chosen IS6110 primers because of multiple copy numbers (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24) in the Mycobacterium genome [8]. MPB64 primer has shown good sensitivity for diagnosis of CNS TB [15].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Multiplex PCR for rapid diagnosis of tuberculous meningitis

Sharma

Ray

et al. 2011

J Neurol

View full text Add to dashboard Cite

Rapid and specific diagnosis of tubercular meningitis is of paramount importance to decrease morbidity and mortality. The aim of the study was to evaluate multiplex PCR using protein b, MPB 64, and IS6110 primers directed against M. tuberculosis complex for the diagnosis of tuberculous meningitis (TBM). Multiplex PCR was performed on 18 TBM confirmed cases (culture was positive), 92 clinically suspected TBM cases and 100 non-TBM (control group) patients. Multiplex PCR had a sensitivity of 94.4% for confirmed cases and specificity of 100% for confirmed TBM cases. In 92 clinically diagnosed but unconfirmed TBM cases, multiplex PCR was positive in 84.78% cases. The overall sensitivity of microscopy, culture and multiplex cases were 1.81, 16.73, and 86.63% and specificity was 100, 100, and 100% respectively. Multiplex PCR using protein b, MPB 64, and IS6110 primers has a high sensitivity and specificity in diagnosis of tubercular meningitis.

show abstract

“…DNA extraction [16] DNA was extracted according to the CTAB-phenol-chloroform extraction method. Briefly, 0.2 ml of CSF was centrifuged at 10,000 rpm for 10 min.…”

Section: Processing Of Csf Samplementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Multiplex PCR for rapid diagnosis of tuberculous meningitis

Sharma

Ray

et al. 2011

J Neurol

View full text Add to dashboard Cite

show abstract

“…Most of the PCR-based studies have used single target such as IS6110 or protein-b for amplification in diagnosis of tuberculosis [6]. Single gene target, however, may result in false negative results as some of these target genes may be absent in some of M. tuberculosis isolates [7,8].…”

Section: Introductionmentioning

confidence: 99%

Does multiplex Polymerase Chain Reaction increase the diagnostic percentage in osteoarticular tuberculosis? A prospective evaluation of 80 cases

Sharma

et al. 2011

International Orthopaedics (SICOT)

Self Cite

View full text Add to dashboard Cite

Purpose Multiplex Polymerase Chain Reaction (MPCR) is a technique in which two or more gene targets are amplified in a single reaction. This has increased sensitivity of diagnosis as a single gene target may be absent in some Mycobacterium tuberculosis strains. Methods MPCR using two target genes specific for Mycobacterium tuberculosis, that is, IS6110 and MPB 64, ZN staining and Mycobacterial culture were performed on synovial fluid/pus samples of 80 (three confirmed, 77 suspected) patients of osteoarticular tuberculosis and 25 non tuberculosis patients. Results MPCR had a sensitivity of 100% in confirmed cases and 81.8% in clinically suspected cases. AFB was positive in one patient and Mycobacterial culture was positive in three patients. MPCR also had 100% specificity; MPB64 was positive in five patients in which IS6110 was negative whereas IS6110 was positive in two patients in which MPB64 was negative.Conclusions MPCR is a sensitive and specific method for diagnosis of paucibacilliary conditions such as osteoarticular tuberculosis.

show abstract

“…10,11 A faster, more sensitive and more specific test for the diagnosis of TBM is urgently needed in clinical practice, and developing improved diagnostic techniques has become a clinical research priority. [12][13][14][15] Mycobacterium tuberculosis is the causative agent of tuberculosis in humans. The M. tuberculosis cell wall is constructed primarily of peptidoglycan, which is bound to arabinogalactan and mycolic acid in a covalent fashion.…”

Section: Introductionmentioning

confidence: 99%

Detection of Delta-like 1 ligand for the diagnosis of tuberculous meningitis: An effective and rapid diagnostic method

Peng

Zhou

et al. 2014

J Int Med Res

View full text Add to dashboard Cite

Objective: To investigate the diagnostic value of Delta-like 1 ligand (DLL1) in cerebrospinal fluid (CSF) and serum, in tuberculous meningitis (TBM). Methods: Patients with a definite diagnosis of central nervous system infection (TBM, viral meningitis/encephalitis or bacterial meningitis) were prospectively enrolled alongside patients with intracranial metastatic tumour and patients with no diagnosis (who served as controls). DLL1 content in CSF and serum was measured quantitatively by enzyme-linked immunosorbent assay; analyses were blinded. Results: A total of 173 patients were enrolled: 62 with TBM; 38 with viral meningitis/encephalitis; 26 with bacterial meningitis; 17 with intracranial metastatic tumour; 30 with no diagnosis. CSF DLL1 content was highest for TBM; there were no differences in CSF DLL1 between the other groups. Serum DLL1 content was highest for the TBM and intracranial metastatic tumour groups, with significant differences between the TBM group and the viral meningitis/encephalitis, bacterial meningitis and nondiagnosed groups. There were no differences in serum DLL1 between the viral meningitis/encephalitis, bacterial meningitis and nondiagnosed groups, or between the TBM group and the tumour group. Conclusion: As a new biomarker, DLL1 may be of great clinical importance in the diagnosis of TBM.

show abstract

Evaluation of polymerase chain reaction using protein b primers for rapid diagnosis of tuberculous meningitis

Abstract: Protein b PCR is valuable in rapid diagnosis of TBM.

Cited by 31 publications

References 14 publications

Multiplex PCR for rapid diagnosis of tuberculous meningitis

Multiplex PCR for rapid diagnosis of tuberculous meningitis

Does multiplex Polymerase Chain Reaction increase the diagnostic percentage in osteoarticular tuberculosis? A prospective evaluation of 80 cases

Detection of Delta-like 1 ligand for the diagnosis of tuberculous meningitis: An effective and rapid diagnostic method

Contact Info

Product

Resources

About