Evaluation of polymerase chain reaction and DNA isolation protocols for detection of genetically modified soybean

Ferrari, Cibele dos Santos; Valente, Luciana Lehmkuhl; Brod, Fábio Cristiano Angonesi; Tagliari, Caroline; Sant’Anna, Ernani Sebastião; Arisi, Ana Carolina Maisonnave

doi:10.1111/j.1365-2621.2006.01405.x

Cited by 21 publications

(14 citation statements)

References 15 publications

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“…Forty samples showed the positive signal for RR detection, an amplicon of 169 bp, confirming the presence of GM soy. For these primer combinations, the sensitivity of the nested PCR was 0.01% (Brod et al ., in press; Ferrari et al ., in press). Regarding to PSm, fifteen out of twenty‐five showed the positive signal for RR soybean (Brod et al.…”

Section: Resultsmentioning

confidence: 99%

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Quantification of Roundup Ready™ soybean in Brazilian soy‐derived foods by real‐time PCR

Brod

Arisi²

2008

Int J of Food Sci Tech

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A significant increase in the growth of genetically modified organisms has been observed in recent years. As a result of the market introduction of these genetically modified (GM) crops in foods, legislation worldwide was prepared to deal with the question of the use and labelling requirements on GM crops and their derivatives. The aim of this work was to detect and to quantify GM soybean in soy-derived food products sold commercially in Floriano´polis, Brazil, by polymerase chain reaction-based analysis. In total sixty-two samples of texturized soy protein and powdered soymilk were analysed and forty samples have been shown to contain Roundup Ready (RR) soy. Quantitative analysis revealed that only two out of these forty samples presented RR soybean content above 1%. These findings confirm the presence of RR soybean in Brazilian soy-derived food products and that the amount of samples with % RR content above 1% decreases when compared with previously published data.

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Section: Resultsmentioning

confidence: 99%

“…These primers were used as described in previous works (Brod et al. , in press; Ferrari et al. , in press).…”

Section: Methodsmentioning

confidence: 99%

Quantification of Roundup Ready™ soybean in Brazilian soy‐derived foods by real‐time PCR

Brod

Arisi²

2008

Int J of Food Sci Tech

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“…DNA was extracted following modified cetyl trimethyl ammonium bromide (CTAB) method (Ferrari et al 2007). CTAB is a standard method for DNA extraction which is followed in most cases.…”

Section: Molecular Characterizationmentioning

confidence: 99%

Morphological and Molecular Characterization of Tropical Strawberry

Bhowal

Hossain

Kayesh

et al. 2019

Plant Tissue Cult. & Biotech.

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The experiment was conducted to assess five tropical strawberry genotypes at phenotypic and molecular level. Among the five strawberry genotypes (BARI Strawberry 1, BARI Strawberry 2, BARI Strawberry 3, FA 005 and Festival), BARI Strawberry 2 was found to be the best in respect of fruit per plant (32.42), fruit yield per plant (594.73 gm) and yield per hectare (19.39 ton). Ten SSR primers were initially screened for molecular characterization and finally MFv104, ARSFL-10 and ARSFL-15 markers were selected for the analysis. EMFv104 and ARSFL-15 produced the maximum number of polymorphic alleles (4) while ARSFL-10 produced three polymorphic alleles. The major allele frequency at each locus ranged from 0.4 (EMFv104) to 0.6 (ARSFL-10). The PIC values varied from 0.4992 on ARSFL-10 to 0.672 on EMFv104. The gene diversity ranged from 0.56 (ARSFL-10) to 0.72 (EMFv104 and ARSFL-15). BARI Strawberry 1 and Festival were the closest genotypes with the lowest genetic dissimilarity value of 0.16667. EMFv104 and ARSFL-15 can be used as polymorphic markers for assessing genetic diversity of different strawberry genotypes.

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“…DNA was extracted from rice seeds of each sample by cetyl trimethyl ammonium bromide (ctab) [8]. The quality of DNA extracted was determined by running an aliquot of 5 µl of each extracted DNA sample in a 1% agarose gel electrophoresis pre-stained with ethidium bromide.…”

Section: Genomic Dna Extraction and Simple Sequence Repeat (Ssr) Analmentioning

confidence: 99%

Genetic Diversity Studies on Selected Rice (Oryza sativa L) Populations Based on Aroma and Cooked Kernel Elongation

Kioko¹,

Musyoki²,

Ngugi³

2015

J Phylogenetics Evol Biol

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Rice (Oryza sativa L.) is the main staple food for more than half of the world's population. Improving cooking and eating quality of rice is one of the important objectives of many plant breeding programs. Aroma and cooked kernel elongation are two critical parameters that determine the market value, cooking and eating qualities of rice. The objective of this study was to evaluate the genetic diversity of thirteen (13) Oryza sativa L. populations from Kenyan and Tanzanian. Genetic diversity was determined using 8 simple sequence repeats (SSR) markers. Diversity data was analyzed using POWERMARKER version 3.25 and GENALEX v 6.5 software packages. The number of alleles per locus ranged from 2 to 4 alleles with an average of 3.12 across 8 loci. The polymorphic information content (pic) ranged from 0.2920 (RM 282) to 0.6409 (RM 339) in all loci with an average of 0.4821. Pair-wise genetic dissimilarity coefficients ranged from 0.1125 to 0.9003 with an average of 0.5312. The average gene diversity over all SSR loci for the 13 rice varieties was 0.6036, ranging from 0.3550 to 0.6391. Maximum genetic similarity was observed between Kilombero and Supa, BS 370 and BS 217. Minimum genetic similarity was observed between Kahogo and BS 217. Cluster analysis was used to group varieties by constructing dendrograms based on SSR data and morphological characterization of grains. The dendrogram based on SSR data formed two distinct clusters of the 13 rice varieties. RM 339 and RM 241 were the most informative markers and could be used for differentiating rice varieties from diverse geographical origins. Results obtained from this study demonstrated that use of trait specific SSR markers can be relied upon in diversity studies among diverse and closely related genotypes. RM 339 and RM 241 markers are recommended for use in diversity studies and in quality assurance for grading of rice varieties. Further analysis should be carried out using a larger number of samples and markers to come up a more conclusive report on the discriminating power of microsatellite markers based on rice grain quality traits.

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Evaluation of polymerase chain reaction and DNA isolation protocols for detection of genetically modified soybean

Cited by 21 publications

References 15 publications

Quantification of Roundup Ready™ soybean in Brazilian soy‐derived foods by real‐time PCR

Quantification of Roundup Ready™ soybean in Brazilian soy‐derived foods by real‐time PCR

Morphological and Molecular Characterization of Tropical Strawberry

Genetic Diversity Studies on Selected Rice (Oryza sativa L) Populations Based on Aroma and Cooked Kernel Elongation

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