Evaluation of Polymorphism in IGF-I and IGFB-3 Genes and their Relationship tith Twinning Rate and Growth Traits in Markhoz Goats

Abstract

growth rate and twinning rate are economic traits that can be used in goat breeding objectives. the aim of this study was to investigate polymorphisms in the insulin-like growth factor 1(IGF-I) and insulin-like growth factor binding protein (IGFBP-3) genes and their relationship with growth traits and twinning in Markhoz goats. Two sets of specific primers were used to amplify a 249bp fragment of IGF-I gene and a 316bp fragment of IGFBP-3 gene. pCr-ssCp analysis revealed three banding patterns for each gene that confirmed presence of a mutation in position 1617 of the IGF-I gene and a mutation in position 58 of IGFBP-3 gene. the genotype frequencies of IGF-I gene were 0.81 (gg), 0.16 (ga) and 0.03 (aa). also, the genotype frequencies of IGFBP-3 gene were 0.79 (TT), 0.17 (TC) and 0.04 (CC). The Odds Ratio estimated for twinning rate was 1.11 for second on first parity, 0.19 for third on first parity and 5.71 for second on third parity. The chi-square statistics were 6.46 for IGF-I gene and 3.32 for IGFBP-3 gene. the results also indicated that different genotypes of these genes had no significant effect on birth weight, weight at 6 months, at 9 months and at 12 months but the interactions between different genotypes of IGF-I and IGFBP-3 genes were significant for weaning weight and average daily gain from birth to weaning. These results suggest that twinning rate in markhoz breed is statistically affected by these genes and can be considered in breeding programs.

Short Communication: Population structure of mangrove crab Scylla oceanica in mangrove ecosystem of Tanjung Lesung, Banten, Indonesia

“…Recent studies have reported an association between SNPs in IGFBP-3 gene with the economic trait on ruminant livestock, including significantly correlated to the birth weight, the body weight of 12, 18 and 24 months (Choudhary et al 2007), weaning weight and ADG from birth to weaning (Rasouli et al 2016), the chest width and chest circumference (Gao et al 2009), and the differences in serum concentrations of IGFBP-3 as a result of the SNP (Choudhary et al 2006). This is due to the significance of IGFBP-3 in the IGF-I regulation; they regulate the amount of the IGF-I to their receptors.…”

“…Eight SNPs have been detected in exon 2 of IGFBP-3 gene, 6 of them produce nonsynonymous protein coding or non-synonymous mutation (Sharma et al 2014) which can lead to a significant metabolic abnormality that is desirable or not desirable (Veneroni-Gouveia et al 2015;de Camargo et al 2015). IGFBP-3 gene expression was higher in the liver and longisimus dorsi muscles, thus contributing to the development of these organs (Zhang et al 2015), increased ADG from birth to weaning and weaning weight (Rasouli et al 2016), but there is not enough evidence to suggest that SNPs are associated with milk production (Zhou et al 2016).…”

“…SNP in the IGFBP-3 gene was reported in Egypt cattle (Othman et al 2014), Holstein and Cross between Holstein-Haryana (Choudhary et al 2007), Hanwoo (Kim et al 2005) and various Chinese cattle (Gao et al 2009;Liu et al 2014). The presence of SNPs in IGFBP-3 gene affected the phenotype appearance, body weight gains (Choudhary et al 2006;Rasouli et al 2016), IGFBP-3 serum concentration (Choudhary et al 2007), body circumference (Gao el at. 2009), and has been used as a candidate gene for meat production (Mahrous et al 2014).…”

“…According to Othman et al (2014), IGFBP-3 can be used as a marker for the function of growth, metabolism, reproduction, immunity and energy balance in cattle. SNP is a condition where the presence of a different nucleotide constituent of a gene, and studies of SNP on IGFBP-3 gene in sheep, goat, cattle and buffalo have been reported (Rodríguez et al 2013;Mahrous et al 2014;Ramesha et al 2015;Rasouli et al 2016). SNP in the IGFBP-3 gene was reported in Egypt cattle (Othman et al 2014), Holstein and Cross between Holstein-Haryana (Choudhary et al 2007), Hanwoo (Kim et al 2005) and various Chinese cattle (Gao et al 2009;Liu et al 2014).…”

Genetic diversity and distance of Iranian goat breeds (Markhoz, Mahabadi and Lori) compared to the Beetal breed using inter-simple sequence repeat (ISSR) markers

“…It seems that a study on major genes known for the twining trait can distinguish S subpopulations from T subpopulations. For example, Rasouli et al (2016) reported that the twinning rate in Markhoz goats was significantly influenced by the IGF-I and IGFBP-3 genotypes; also, the association of two single nucleotide polymorphisms (SNPs) in the growth hormone (GH) gene with the litter size and superovulation response in goat breeds has been studied, and the results have shown that the two loci of the GH gene are highly associated with abundant prolificacy and a superovulation response in goat breeds (Zhang et al, 2011).…”