This research aims to evaluate the quality of goat feces liquid biofertilizer with chicken excreta addition by aerobic and anaerobic fermentation. The data obtained from the research were statistically analyzed with Completely Randomized Design (CRD) Factorial Pattern with two factors. The first factor was addition level of the chicken excreta (0, 10, 20, and 30%) and the other factor was fermentation treatment (aerobic and anaerobic) condition with 3 replications for each treatment. Fermentation was conducted for 14 days. The observed parameters consisted of the chemical parameter (organic-C, N, P, and K) and the physical parameter (pH, temperature, and the odor). The addition of various level of chicken excreta and fermentation treatments in the process of producing goat feces liquid biofertilizer indicate significantly effect to increase the organic-C, total-N, NH4, P, K, compare with 5 and 10% liquid fertilizer treatment. The best quality of liquid biofertilizer produced by combining aerobic fermentation and the addition of 30% chicken excreta. Generally, chicken excreta able to increase the nitrogen and mineral content, and putting together with aerobic fermentation system of chicken excreta maximum to 30% can avoid overproduction of unpleasant odor.
Abstract. Priyadi DA, Panjono, Bintara S, Hartatik T. 2017. The genotype of gene sequences. Biodiversitas 18: 795-800. The IGFBP-3 gene is a potential marker of cattle growth, which is related to the functions that influence the growth, energy metabolism, reproduction and immunity. The genome was isolated from whole blood samples of 10 Brahman cattle and 16 Brahman Cross cattle. Cattle IGFBP-3 gene that targeted in this study was located in the part of intron 2, exon 3 and part of intron 3. The gene targets were amplified using specific primers by Polymerase Chain Reaction (PCR) technique, resulting 563 bp amplification product. As data comparison to reveal the SNP, the GenBank sequences (n = 14) from various breed and countries were used. The objective of this study was to reveal the SNP on the Brahman and Brahman Cross IGFBP-3 gene. The results obtained 3 genotypes from one SNP that spread in the sample population. The SNP was located in intron 2 at position 3,930 (GA). The Polymorphism could be recognized by PvuII restriction enzymes. There was not enough evidence to associate the SNP with the phenotype (at pre-weaning age) of Brahman and Brahman Cross cattle. There was a genetic diversity in the population studied. Knowledgeable SNP could be used as genetic markers for further research on Brahman and Brahman Cross cattle.
This study focused on the promoter region of the SRY gene with 1,281 bp DNA fragments, including 5'UTR, CAAT signal, and TATA box. Genomic samples of 19 cattle were obtained from Wagyu-BX (n = 2), BX (n = 5), Simmental (n = 2), Limousin (n = 2), Ongole (n = 2), Madura (n = 2), Bali (n = 2), Nellore (n = 1), and Hereford (n = 1). Two flanking primers (forward and reverse) were used for polymerase chain reaction (PCR). The PCR products were then sequenced by using a two-way primer. The obtained sequences were aligned with clustalW software to determine the differences in the nucleotide base arrangement which compiled the promoter region of the SRY gene. The cattle crossbreeding was done as an effort to improve the genetic variations and qualities. The SRY gene is a marker gene inherited from the male side (bull), so the SRY gene is expected to be used as a marker to monitor the crossbreeding. The monitoring of the crossbreed cattle is an initial effort to increase the genetic variations and enhance the genetic qualities without threatening the germplasm purity. The results of this study showed that the overall sample is monomorphic, except for Bali and Nellore cattle. Further research is needed by expanding the analysis area of the SRY gene and increasing the number of samples.
Penelitian ini bertujuan untuk mengetahui pengaruh penambahan sari edamame terhadap kualitas kualitas kimia (pH, kadar air, kadar abu, kadar protein, dan kadar lemak) serta aktivitas antioksidan pada eggurt. Penambahan edamame pada eggurt dimaksudkan untuk meningkatkan kandungan kimia serta aktivitas antioksidan karena sari edamame mengandung flavonoid dan fenol yang tinggi. Pembuatan eggurt dengan memfermentasi susu murni dan putih telur menggunakan bakteri BAL berupa Lactabacillus bulgaricus dan Streptococcus thermophilus kemudian diinkubasi pada suhu 42ºC sampai membentuk curd atau nilai pH mencapai 4,5. Eggurt dibuat dengan menambahkan sari edamame sesuai dengan perlakuan. Perlakuan yang digunakan yaitu P1 (sari edamame 5%), P2 (sari edamame 10%), P3 (sari edamame 15%), dan P4 (sari edamame 20%). Selanjutnya data dianalisis menggunakan analisis varian (ANOVA). Apabila terdapat beda nyata, maka dilanjutkan dengan uji Duncan dengan taraf 1%. Hasil penelitian menunjukkan penambahan edamame berpengaruh sangat nyata (P<0,01) terhadap kadar air, kadar abu, kadar protein, dan kadar lemak eggurt, dan aktivitas antioksidan sedangkan tidak berpengaruh nyata (P>0.05) terhadap parameter pH pada eggurt. Hasil penelitian dapat disimpulkan bahwa penambahan sari edamame pada eggurt dengan kosentrasi 20% berpengaruh dengan meningkatkan nilai kadar air, kadar protein, kadar lemak, dan antioksidan, serta menurunkan nilai kadar abu, namun tidak berpengaruh terhadap nilai pH.
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