Evaluation of Pooled and individual components ofBordetella pertussis as antigens in an enzyme immunoassay for diagnosis of pertussis

He, Qiushui; Mertsola, Jussi; Himanen, Juha P.; Ruuskanen, Olli; Viljanen, Matti K.

doi:10.1007/bf02009381

Cited by 21 publications

(8 citation statements)

References 23 publications

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“…Like Mertsola et al , 10 , 11 , 12 , 13 we found a high level of pre‐existing placentally acquired antipertussis IgG, which was not unexpected during a period in which pertussis was epidemic. This affects the magnitude of the vaccine response, as measured in the whole‐cell ELISA assay if only the fold‐increase in IgG index is considered.…”

Section: Discussionsupporting

confidence: 81%

“…Few studies have been published on the use of whole‐cell ELISA to measure pertussis antibodies. Mertsola and colleagues 10 , 11 , 12 , 13 have assessed the performance of whole‐cell ELISA in a number of pertussis outbreaks and have compared that assay with single‐antigen assays and with ELISA assays that use a pool of purified antigens. Their conclusion was that the antipertussis IgG measured in a whole‐cell ELISA is quite likely to be high in the ‘acute’ phase blood sample of vaccinated individuals, and that IgG seroconversions are therefore not commonly detected, even if there is a rise in pertussis IgG antibodies.…”

Section: Discussionmentioning

confidence: 99%

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Pertussis antibody levels in infants immunized with an acellular pertussis component vaccine, measured using whole‐cell pertussis ELISA

et al. 2000

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Summary A commercially available whole-cell pertussis IgG ELISA was used to test the response of 137 2-month-old infants to immunization with a trivalent acellular pertussis vaccine. The pre-immunization geometric mean (GM) IgG index was 6.96 (95% confidence interval (CI) 5.88-8.04) and the postimmunization GM index was 13.16 (95% CI 12.20-14.11), P < 0.001. Eighty percent of subjects (110/137) had a significant 1.5-fold increase of pertussis IgG index (97/137, 71%) or a postimmunization IgG index > 10 (93/137, 68%). In single antigen ELISA, 83% showed at least a fourfold increase in pertussis toxin-specific IgG (PT-IgG) and 91% showed an increase in IgG specific for filamentous haemagglutinin (FHA-IgG). Four percent had high preimmunization antibody levels (index > 20), likely to reflect recent maternal exposure to pertussis. This correlated with a smaller increase in pertussis IgG index. A decline in pertussis IgG index postimmunization occurred in 17/24 infants (71%) whose pre-immunization IgG index was > 10. This postimmunization pertussis IgG index was not significantly different to that of infants with a low pre-immunization index. A similar trend was noted with PT-IgG and FHA-IgG results. The whole-cell ELISA can detect a response to acellular pertussis vaccination in most infants if both antibody index and degree of seroconversion are calculated and at least one criterion is satisfied.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Pertussis antibody levels in infants immunized with an acellular pertussis component vaccine, measured using whole‐cell pertussis ELISA

et al. 2000

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“…Mostly, these ELISAs use purified antigens, although the use of mixed antigens has also been described (8,13,18). The availability of an international reference preparation for pertussis serology has increased the comparability between ELISAs (24).…”

Section: Discussionmentioning

confidence: 99%

Performance of Commercial Enzyme-Linked Immunosorbent Assays for Detection of Antibodies to Bordetella pertussis

et al. 2010

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Measuring antibodies to Bordetella pertussis antigens is mostly done by enzyme-linked immunosorbent assays (ELISAs). We compared the performance of ELISA kits that were commercially available in Germany. Eleven measured IgG antibodies, and nine measured IgA antibodies. An in-house ELISA with purified antigens served as a reference method. Samples included two WHO reference preparations, the former Food and Drug Administration (FDA)/Center for Biologics Evaluation and Research (CBER) reference preparations, serum samples from patients with clinically suspected pertussis, and serum samples from patients having received a combined tetanus, diphtheria, and pertussis (Tdap) vaccination. Kits using pertussis toxin (PT) as an antigen showed linearity compared to the WHO Reference preparation (r 2 between 0.82 and 0.99), and these kits could quantify antibodies according to the reference preparation. ELISA kits using mixed antigens showed no linear correlation to the reference preparations. Patient results were compared to results of in-house ELISAs using a dual cutoff of either >100 IU/ml anti-PT IgG or >40 IU/ml anti-PT IgG together with >12 IU/ml anti-PT IgA. The sensitivities of kits measuring IgG antibodies ranged between 0.84 and 1.00. The specificities of kits using PT as an antigen were between 0.81 and 0.93. The specificities of kits using mixed antigens were between 0.51 and 0.59 and were thus not acceptable. The sensitivities of kits measuring IgA antibodies ranged between 0.53 and 0.73, and the specificities were between 0.67 and 0.94, indicating that IgA antibodies may be of limited diagnostic value. Our data suggest that ELISAs should use purified PT as an antigen and be standardized to the 1st International Reference preparation.

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“…The study subjects included 125 patients (50 males; age range 1–71 years; median, 15 years; age groups: ≤10 years n = 38, 11–18 years n = 38, and >18 years n = 49) randomly selected from serologically diagnosed pertussis patients at the Department of Medical Microbiology and Immunology, the University of Turku in 2004 (Table ). The diagnosis was based on the IgM and IgA antibody levels measured by ELISA using sonicated B. pertussis bacteria as a coating antigen . The diagnosis was further confirmed with a pertussis toxin (PT)‐based ELISA at our Pertussis Reference Laboratory, National Institute of Health and Welfare, Turku, Finland.…”

Section: Methodsmentioning

confidence: 99%

Increased risk of pertussis in adult patients with mannose‐binding lectin deficiency

Gröndahl-Yli-Hannuksela

Viander

Mertsola

et al. 2012

APMIS

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Mannose-binding lectin (MBL) is an important molecule of the innate immunity. The low level of MBL in the serum is associated with increased susceptibility to respiratory infections. In this study, MBL concentrations were determined from the sera of 125 Finnish pertussis patients and from 430 control subjects. Severe MBL deficiency (<50 ng/mL) was found more often in the patients than in the controls (11.2% vs 5.8%, p = 0.038). Moreover, the deficiency was detected more frequently in the adult patients than in the controls [20.4% vs 8.6%, p = 0.021; odds ratio 2.7 (95% confidence interval 1.1-6.5)]. Our findings suggest, for the first time, that MBL deficiency predisposes to pertussis infection, at least in adults.

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Evaluation of Pooled and individual components ofBordetella pertussis as antigens in an enzyme immunoassay for diagnosis of pertussis

Cited by 21 publications

References 23 publications

Pertussis antibody levels in infants immunized with an acellular pertussis component vaccine, measured using whole‐cell pertussis ELISA

Pertussis antibody levels in infants immunized with an acellular pertussis component vaccine, measured using whole‐cell pertussis ELISA

Performance of Commercial Enzyme-Linked Immunosorbent Assays for Detection of Antibodies to Bordetella pertussis

Increased risk of pertussis in adult patients with mannose‐binding lectin deficiency

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