Evaluation of potential environmental toxicity of polymeric nanomaterials and surfactants

Hering, Indra; Eilebrecht, Elke; Parnham, Michael J.; Günday-Türeli, Nazende; Türeli, Akif Emre; Weiler, Marc; Schäfers, Christoph; Fenske, Martina; Wacker, Matthias G.

doi:10.1016/j.etap.2020.103353

Cited by 30 publications

(16 citation statements)

References 65 publications

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“…We screened a panel of surfactants (data not shown) and discovered that Pluronic F-68 did not enhance the endogenous RNase activity present in saliva samples and demonstrated that the addition of 0.2% Pluronic F-68 to the sample preparation buffer increased the sensitivity of the RT-LAMP assay. Pluronic F-68 is an environmentally friendly [48], non-ionic detergent, commonly used to reduce foaming in stirred cultures and reduce cell attachment to glass. Detergents dissolve the lipid bilayer by forming a micelle, a process that depends on temperature and the critical micelle concentration (CMC).…”

Section: Discussionmentioning

confidence: 99%

Development and Implementation of a Simple and Rapid Extraction-Free Saliva SARS-CoV-2 RT-LAMP Workflow for Workplace Surveillance

Bruce

Cohen

et al. 2022

Preprint

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Effective management of the COVID-19 pandemic requires widespread and frequent testing of the population for SARS-CoV-2 infection. Saliva has emerged as an attractive alternative to nasopharyngeal samples for surveillance testing as it does not require specialized personnel or materials for its collection and can be easily provided by the patient. We have developed a simple, fast, and sensitive saliva-based testing workflow that requires minimal sample treatment and equipment. After sample inactivation, RNA is quickly released and stabilized in an optimized buffer, followed by reverse transcription loop-mediated isothermal amplification (RT-LAMP) and detection of positive samples using a colorimetric and/or fluorescent readout. The workflow was optimized using 1,670 negative samples collected from 172 different individuals over the course of 6 months. Each sample was spiked with 50 copies/μL of inactivated SARS-CoV-2 virus to monitor the efficiency of viral detection. Using pre-defined clinical samples, the test was determined to be 100% specific and 97% sensitive, with a limit of detection comparable to commercially available RT-qPCR-based diagnostics. The method was successfully implemented in a CLIA laboratory setting for workplace surveillance and reporting. From April 2021-February 2022, more than 30,000 self-collected samples from 755 individuals were tested and 85 employees tested positive mainly during December and January, consistent with high infections rates in Massachusetts and nationwide. The rapid identification and isolation of infected individuals with trace viral loads before symptom onset minimized viral spread in the workplace.

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Section: Discussionmentioning

confidence: 99%

Development and Implementation of a Simple and Rapid Extraction-Free Saliva SARS-CoV-2 RT-LAMP Workflow for Workplace Surveillance

Bruce

Cohen

et al. 2022

Preprint

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“…2018; Hering et al . 2020). These effects could be related to NPs agglomeration onto the surface chorion and the consequent hypoxia induction.…”

Section: Challenges and Perspectivesmentioning

confidence: 99%

Developing oral nanovaccines for fish: a modern trend to fight infectious diseases

Angulo

Tello‐Olea

Reyes‐Becerril

et al. 2020

Reviews in Aquaculture

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Nanovaccines constitute an innovative approach in vaccinology, enabled by the nanotechnology advances on the synthesis, functionalization and application of nanoparticles for immunization. Currently, several organic materials such as chitosan, poly(lactic-co-glycolic acid), nanopoliplexes and virus-like particles (VLPs) have been used for nanovaccine development to fight against fish diseases. The oral route is the most practical and economical for fish vaccination; thus, several oral vaccine candidates have been developed by using NPs in which the antigen is encapsulated, surface exposed or recombinantly ensembled (VLPs), leading to promising results in terms of protection capacity against infectious diseases. In this review, the state of the art on oral nanovaccine prototypes developed for use in fish was analysed and discussed. Oral nanovaccines have showed remarkably possibilities for application in fish aquaculture, which surely will be adopted by the industry in the following years.

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“…Therefore, by reducing or replacing these inactive ingredients in the formulation, a reduction in the emission of ecotoxins is expected. The polymer Eudragit ® RLPO has a positive surface charge and can more easily be extracted from wastewater [ 18 , 19 ]. Therefore, the current formulation design contributes to the more sustainable use of excipients in the pharmaceutical industry.…”

Section: Introductionmentioning

confidence: 99%

Sustainable Stabilizer-Free Nanoparticle Formulations of Valsartan Using Eudragit® RLPO

Hajba-Horváth

Fodor-Kardos

Shah

et al. 2021

IJMS

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The bioavailability of the antihypertensive drug valsartan can be enhanced by various microencapsulation methods. In the present investigation, valsartan-loaded polymeric nanoparticles were manufactured from Eudragit® RLPO using an emulsion–solvent evaporation method. Polyvinyl alcohol (PVA) was found to be a suitable stabilizer for the nanoparticles, resulting in a monodisperse colloid system ranging in size between 148 nm and 162 nm. Additionally, a high encapsulation efficiency (96.4%) was observed. However, due to the quaternary ammonium groups of Eudragit® RLPO, the stabilization of the dispersion could be achieved in the absence of PVA as well. The nanoparticles were reduced in size (by 22%) and exhibited similar encapsulation efficiencies (96.4%). This more cost-effective and sustainable production method reduces the use of excipients and their expected emission into the environment. The drug release from valsartan-loaded nanoparticles was evaluated in a two-stage biorelevant dissolution set-up, leading to the rapid dissolution of valsartan in a simulated intestinal medium. In silico simulations using a model validated previously indicate a potential dose reduction of 60–70% compared to existing drug products. This further reduces the expected emission of the ecotoxic compound into the environment.

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Evaluation of potential environmental toxicity of polymeric nanomaterials and surfactants

Cited by 30 publications

References 65 publications

Development and Implementation of a Simple and Rapid Extraction-Free Saliva SARS-CoV-2 RT-LAMP Workflow for Workplace Surveillance

Development and Implementation of a Simple and Rapid Extraction-Free Saliva SARS-CoV-2 RT-LAMP Workflow for Workplace Surveillance

Developing oral nanovaccines for fish: a modern trend to fight infectious diseases

Sustainable Stabilizer-Free Nanoparticle Formulations of Valsartan Using Eudragit® RLPO

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