Evaluation of potential genetic and chemical markers for Scots pine tolerance against Heterobasidion annosum infection

Mukrimin, Mukrimin; Kovalchuk, Andriy; Ghimire, Rajendra P.; Kivimäenpää, Minna; Sun, Hui; Holopainen, Jarmo K.; Asiegbu, Fred O.

doi:10.1007/s00425-019-03270-8

Cited by 28 publications

(28 citation statements)

References 52 publications

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“…The mortality differences of trees in the studies may be determined by different family susceptibilities to the pathogen (Swedjemark et al 2001;Mukrimin et al 2019) unequal pathogen virulence (Lakomy et al 2011), incubation period (Stenlid and Swedjemark 1988;Zaluma et al 2016), and plant defense mechanisms (i.e., secondary metabolites, phenolic compounds, terpenoid compounds) (Hammerschmidt 2005; Witzell and Martín 2008;Chong et al 2009;Roach et al 2014). The variation between different provenances and host genotype was reported for several stilbenes, flavonoids and terpenoids (Slimestad 1998;Evensen et al 2000;Ganthaler et al 2017b;Mukrimin et al 2019). Our results indicate that the half-sib families with a higher resistance index are characterized by higher TPC concentration.…”

Section: Discussionmentioning

confidence: 99%

“…A significant and high correlation coefficient was determined between the resistance index and TPC accumulation in Scots pine half-sib families. The findings of Mukrimin et al (2019) revealed that Scots pine trees with low concentration of individual terpenoids (β-caryophyllene and α-humulene) were characterized by the greatest susceptibility to H. annosum necrosis. Data analysis of our research has shown the suitability of the chosen method of application of chemical resistance (Total Phenolic Content (TPC) accumulation in trees) for identification of root rot-resistant genotypes, which can be used as an additional indicator to determine resistance of trees to H. annosum.…”

Section: Discussionmentioning

confidence: 99%

“…Karst.) and Scots pine genotypes were determined in experiments where these tree species were inoculated with H. annosum (Von Weissenberg 1975;Swedjemark and Karlsson 2004;Mukrimin et al 2019). Skrøppa et al (2015a) noted, that up to ~35% of this variation can be assigned to genotypic variation.…”

Section: Introductionmentioning

confidence: 99%

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Resistance of Scots pine half-sib families to <i>Heterobasidion annosum</i> in progeny field trials

Marčiulynas¹,

Sirgedaitė-Šėžienė²,

Žemaitis³

et al. 2020

Silva Fenn.

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Five Scots pine ( L.) progeny field trials, each established in different Lithuanian regions of provenance in 1983, were studied. Each progeny field trial consists of 140 half-sib families from seven populations (20 families from each population). The evaluation was carried out in 2012 and 2018 to assess the families resistance to (Fr.) Bref. An index of resistance in the infected plots was calculated. To verify the accuracy of the method, total phenolic compounds (TPC) was chosen as key parameter to compare with the plant resistance index. During the six years between the two assessments, the percentage of living Scots pine trees in the progeny field trials decreased up to 20 percentage points (range: 4 p.p. to 20 p.p.). In 2018 the area of damaged plots (in percentage from total field trial area) varied from 17 to 27%. Tree mortality in the trial correlates with site soil fertility â more fertile soils were distinguished by higher tree percentage loss and . Using analysis from combined data of all progeny trials, the family variance component reached 13.3âÂ±â2.2% and family heritability was 0.81. Family heritability estimates for root rot resistance show possibilities of high breeding effectiveness. The correlations between the trials in family resistance estimates were negligible (ranging from 0 to 0.28). The significant high correlation coefficient was determined between the resistance index and TPC concentration (â=â0.77, â=â0.0003). This allows us to assume that plant resistance is directly linked on TPC synthesis. The results indicate that the chosen methods of chemical resistance for identification of root rot-resistant genotypes are applicable for the selection of Scots pine half-sib families in the field trials with higher resistance to pathogens.Pinus sylvestrisHeterobasidion annosumH. annosumvice versarp

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Resistance of Scots pine half-sib families to <i>Heterobasidion annosum</i> in progeny field trials

Marčiulynas¹,

Sirgedaitė-Šėžienė²,

Žemaitis³

et al. 2020

Silva Fenn.

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“…Genetic control of resistance has been previously detected in P. sylvestris [10], and clones with apparently varying resistance have been detected in seed orchards [11] and among seedlings [12]. At the genetic level, a recent study about terpenoids associated with P. sylvestris defensive mechanisms against H. annosum has indicated higher expression of genes encoding certain terpenoid compounds, thus showing potential to identify susceptibility of trees based on inherent genetic and chemical properties [13]. Moreover, copy number variation polymorphism had been detected for a gene encoding a thaumatin-like protein, involved in antimicrobial activity against 12 fungal species.…”

Section: Introductionmentioning

confidence: 99%

Pinus sylvestris Breeding for Resistance against Natural Infection of the Fungus Heterobasidion annosum

Rieksts-Riekstiņš

Zeltiņš

Baliuckas

et al. 2019

Forests

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Increasing resistance against biotic and abiotic factors is an important goal of forest tree breeding. The aim of the present study was to develop a root rot resistance index for Scots pine breeding and evaluate its effectiveness. The productivity, branch diameter, branchiness, stem straightness, spike knots, and damage from natural infection of root rot in 154 Scots pine open-pollinated families from Latvia were evaluated through a progeny field trial at the age of 38 years. Trees with decline symptoms were sampled for fungal isolations. Based on this information and kriging estimates of root rot, 35 affected areas (average size: 108 m 2 ; total 28% from the 1.5 ha trial) were delineated. Resistance index of a single tree was formed based on family adjusted proportion of live to infected trees and distance to the center of affected area. Heritability for resistance to root rot based on the value of this index, was high (0.37) and comparable to indices of growth traits. Correlations of family breeding estimates between resistance to root rot and the other traits were not significant, except for a weak, yet significant, positive correlation with diameter at breast height and branch diameter. Selection index including only growth traits (height and stem volume) had a negligible effect on damage by root rot. We detected a maximum genetic gain in resistance index of 33.7% when incorporating it into the selection index with positive gains for growth traits (6.5-11.0%). Two-stage selection with prior selection of the most resistant families was not superior to the use of selection index with only rot resistance included. Overall; rot resistance index appeared to be an effective tool in tree breeding for the selection of more resistant families, using the existing trials with natural (uncontrolled) infection

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“…Для селекції стійкості сосни звичайної до хвороби на генетичному рівні виявлено потенційні біомаркери -два терпенові з'єднання і чотири гени. Таким чином, стійкість дерев можна ідентифікувати на основі властивих їм генетичних і хімічних властивостей (Mukrimin et al, 2019).…”

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Hereditary characters resistance to Heterobasidion annosum resistance of pine seedlings grown from tree seeds in the disease foci

Ustskiy¹,

Mikhailichenko²,

Dyshko³

2020

Ukr. j. for. wood sci.

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Український науково-дослідний інститут лісового господарства агролісомеліорації імені Г. М. Висоцького Чисті соснові насадження, що створені на землях, які перебували у сільськогосподарському користуванні, як правило, уражуються кореневими гнилями, збудником яких є гриб класу базидіоміцети-коренева губка (Heterobasidion annosum (Fr.) Bref). Радикальних методів боротьби з хворобою, незважаючи на більш ніж 200-річний період досліджень у різних країнах світу, немає до цього часу. Тому альтернативним напрямом розв'язання цієї проблеми є селекція сосни на стійкість до хвороби. Метою дослідження було вивчення біометричних характеристик репродуктивного та садивного матеріалу дерев сосни звичайної з різною стійкістю до кореневої губки. Дослідження проводили в чистому 60-річному сосновому насадженні, ураженому кореневою губкою, в умовах Харківської області. Результати дослідження біометричних показників репродуктивних органів дерев сосни звичайної з різною стійкістю до кореневої губки свідчать, що дерева з підвищеною резистентністю характеризуються більшою середньою масою шишки, порівняно із ураженими і неураженими хворобою деревами, та близькою до неуражених дерев масою 1000 шт. насіння. Біометричні характеристики однорічних сіянців сосни, вирощених у теплиці з насіння «хворих», «здорових» і «стійких» дерев, засвідчили, що характерною ознакою однорічного потомства стійких дерев в осередках кореневої губки є суттєво довша коренева система сіянців, що, вірогідно, і є основним критерієм стійкості сосни до хвороби. На нашу думку, при створенні насаджень, стійких до кореневої губки, потрібно використовувати 20-30 % садивного матеріалу, вирощеного з насіння дерев підвищеної резистентності. В подальшому, з метою вирощування стійких до кореневої губки соснових насаджень, відбірні сіянці з потомства стійких дерев рекомендовано використовувати при створенні соснових насаджень на староорних землях, для заліснення зрубів уражених кореневою губкою насаджень і прогалин в осередках всихання.

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Evaluation of potential genetic and chemical markers for Scots pine tolerance against Heterobasidion annosum infection

Abstract: Main conclusion Two terpene compounds and four genes were identified as potential biomarkers for further evaluation for Scots pine susceptibility or tolerance against Heterobasidion annosum.

Cited by 28 publications

References 52 publications

Resistance of Scots pine half-sib families to <i>Heterobasidion annosum</i> in progeny field trials

Resistance of Scots pine half-sib families to <i>Heterobasidion annosum</i> in progeny field trials

Pinus sylvestris Breeding for Resistance against Natural Infection of the Fungus Heterobasidion annosum

Hereditary characters resistance to Heterobasidion annosum resistance of pine seedlings grown from tree seeds in the disease foci

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