Evaluation of Pressed Powders and Thin Section Standards for Multi-Elemental Analysis by Conventional and Micro-Pixe Analysis

Homma-Takeda, Shino; Iso, Hiroyuki; Ito, Masaki; Suzuki, Kyoko; Harumoto, Keiko; Yoshitomi, Tomoyasu; Ishikawa, Takahiro; Oikawa, Masakazu; Suya, Noriyoshi; Konishi, Teruaki; Imaseki, Hitoshi

doi:10.1142/s0129083510001926

Cited by 9 publications

(6 citation statements)

References 16 publications

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“…Uranium in areas of the S3 segments with and without concentrated phosphorus were quantified by μ-PIXE spot analysis using the μ-PIXE system with a CdTe detector [23]. Briefly, X-ray intensity data of the U Lβ2 were obtained by scanned 1 μm × 1 μm areas of the specimens under conditions of proton energy of 3.0 MeV and integrated current of 0.2 μC with 1 μm × 1 μm spatial resolution.…”

Section: Methodsmentioning

confidence: 99%

Phosphorus Localization and Its Involvement in the Formation of Concentrated Uranium in the Renal Proximal Tubules of Rats Exposed to Uranyl Acetate

Homma-Takeda

Numako

Kitahara

et al. 2019

IJMS

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Although the kidneys comprise a critical target of uranium exposure, the dynamics of renal uranium distribution have remained obscure. Uranium is considered to function physiologically in the form of uranyl ions that have high affinity for phosphate groups. The present study applied microbeam-based elemental analysis to precisely determine the distribution of phosphorus and uranium in the kidneys of male Wistar rats exposed to uranium. One day after a single subcutaneous injection of uranyl acetate (2 mg/kg), areas of concentrated phosphorus were scattered in the S3 segments of the proximal tubule of the kidneys, whereas the S3 segments in control rats and in rats given a lower dose of uranium (0.5 mg/kg) contained phosphorus without concentrated phosphorus. Areas with concentrated phosphorus contained uranium 4- to 14-fold more than the mean uranium concentration (126–472 vs. 33.1 ± 4.6 μg/g). The chemical form of uranium in the concentrated phosphorus examined by XAFS was uranium (VI), suggesting that the interaction of uranyl ions with the phosphate groups of biomolecules could be involved in the formation of uranium concentration in the proximal tubules of kidneys in rats exposed to uranium.

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Section: Methodsmentioning

confidence: 99%

Phosphorus Localization and Its Involvement in the Formation of Concentrated Uranium in the Renal Proximal Tubules of Rats Exposed to Uranyl Acetate

Homma-Takeda

Numako

Kitahara

et al. 2019

IJMS

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“…Cellular imaging of uranium and endogenous elements was performed using a Model OM-2000 microbeam scanning PIXE system (Oxford Microbeams Ltd., Oxford, UK) with a Si (Li) detector for light elements and a CdTe detector for uranium [27]. Elemental images were constructed using the intensity data of the P Kα, S Kα, Cl Kα, K Kα, and U Lβ1 lines at each point, which were obtained by scanning the specimens under the following conditions: proton energy, 3.0 MeV, integrated charge, 0.2 μC, and spatial resolution, 1 μm × 1 μm.…”

Section: μ-Pixe Analysismentioning

confidence: 99%

Single-Cell Imaging for Studies of Renal Uranium Transport and Intracellular Behavior

et al. 2021

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Nephrotoxicity is the primary health effect of uranium exposure. However, the renal transport and intracellular behavior of uranium remains to be clearly elucidated. In the present study, the intracellular uranium distribution was examined with the cell lines derived from the S3 segment of mouse renal proximal tubules, which is a toxic target site of uranium, using microbeam-based elemental analysis. Uranium exposure at 100 μM for 24 h (non-toxic phase) was performed in S3 cells. Two types of measurement specimens, including those that are adhesive cell specimens and cryosection specimens, were examined for the positional relationship of the intracellular localization of uranium. Based on the combined results of single-cell imaging from the two types of cell specimens, uranium was distributed inside the cell and localized in the cytoplasm near the cell nucleus. In some cells, uranium was colocalized with phosphorus and potassium. The amount of uranium accumulated in S3 cells was estimated using thin section-standards. The mean uranium content of three adhesive cells was hundreds of femtogram per cell. Thus, we believe that single-cell imaging would be useful for studies on renal uranium transportation and cellular behavior.

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“…The uranium concentration range was calculated using thin-section standards. [285][286][287] The periphery of the renal cortex is shown on the upper side. Uranium was localized to the inner cortex and the outer stripes of the outer medulla, where TUNEL-positive tubules were also observed.…”

Section: Future Research Perspectivesmentioning

confidence: 99%

The key role of atomic spectrometry in radiation protection

Zheng¹,

Tagami²,

Homma-Takeda³

et al. 2013

J. Anal. At. Spectrom.

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Evaluation of Pressed Powders and Thin Section Standards for Multi-Elemental Analysis by Conventional and Micro-Pixe Analysis

Cited by 9 publications

References 16 publications

Phosphorus Localization and Its Involvement in the Formation of Concentrated Uranium in the Renal Proximal Tubules of Rats Exposed to Uranyl Acetate

Phosphorus Localization and Its Involvement in the Formation of Concentrated Uranium in the Renal Proximal Tubules of Rats Exposed to Uranyl Acetate

Single-Cell Imaging for Studies of Renal Uranium Transport and Intracellular Behavior

The key role of atomic spectrometry in radiation protection

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