Evaluation of primary mediastinal large B cell lymphoma by flow cytometry

Abstract

FC can detect the NP of PMLBCL and a FC assay for CHL performed better than a B-NHL assay in this regard. The neoplastic cells showed a B cell immunophenotype with over-expression of CD40. The reactive infiltrate in PMLBCL shows unique features including a prominent CD8 T cell infiltrate. © 2017 International Clinical Cytometry Society.

A comparison and review of the flow cytometric findings in classic Hodgkin lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, T cell/histiocyte rich large B cell lymphoma, and primary mediastinal large B cell lymphoma

“…When applied to PMLBCL, the CHL flow cytometry combination in Table 1 allowed for the detection of the neoplastic population in all 24 cases examined (100%), while our standard flow cytometry combination for B cell non-Hodgkin lymphoma detected the neoplastic population in 18 of 24 cases (75%) (Cherian & Fromm, 2018). A two tube-flow cytometry tube combination ( misclassified) for NLPHL (Figure 4) (Fromm et al, 2017).…”

“…With the possible exception of PMLBCL, these lymphomas have not been amenable to immunophenotypic characterization by flow cytometry, perhaps due to the rarity and lability of the neoplastic cells. However, over the last 15 years, our laboratory has developed flow cytometry combinations to immunophenotype of the neoplastic cells of all four of these B-cell lymphomas (Cherian & Fromm, 2018;Fromm et al, 2006;Fromm et al, 2009;Fromm et al, 2017;Fromm & Wood, 2014;Glynn & Fromm, 2020). These flow cytometric studies augment the description of protein expression that have been observed by immunohistochemistry in these lymphomas.…”

Issue Highlights—May 2018 (94B3)

“…Building on Jonathan Fromm's previous work diagnosing classical Hodgkin's lymphoma by flow cytometry Sindhu Cherian and Jonathan Fromm extend their finding with a flow cytometric diagnostic tube for Hodgkin's lymphoma to include the detection of primary mediastinal large B cell lymphoma (PMLBCL). PMLBCL is a B cell non‐Hodgkin lymphoma with immunophenotypic similarities to classical Hodgkin's lymphoma.…”

From the Editor: Thank you! Remembrances and Issue Highlights

“…The initial contribution by Seegmiller, His and Craig [(3) & cover article] is a review of flow cytometry in the evaluation of mature B-cell neoplasms, a topic of regular interest to our readership as evidenced by these recent publications (3)(4)(5)(6)(7)(8). This is a joint effort of the ICCS and our Society's own Sa Wang and the Society of Hematopathology, represented by Magdalena Czader.…”

“…Wang and Czader are the Guest Editors of this series that will ultimately be collated and published by John Wiley as an open-access electronic book. The initial contribution by Seegmiller, His and Craig [(3) & cover article] is a review of flow cytometry in the evaluation of mature B-cell neoplasms, a topic of regular interest to our readership as evidenced by these recent publications (3)(4)(5)(6)(7)(8). The review by Drs.…”